[PDF] Top 20 Selection and Preliminary Characterization of Temperature-Sensitive Mutants of Type 5 Adenovirus

... Preliminary biochemical and immunological characterization of the mutants indicated that: i the single mutant in complementation group I did not replicate its deoxyribonucleic acid DNA o[r] ... See full document

... These data again reveal that i AAV DNA synthesis does not detectably occur in the absence of helper, ii there is a marked restriction of adenovirus DNA synthesis by the ts mutants at 39.[r] ... See full document

... Rabbit antiserum containing specific 100K protein antibodies was used for indirect immunofluorescence examination of cells infected with wild-type virus, 100K mutants, and hexon mutants.[r] ... See full document

... Immunofluorescence examination of cells infected with wild-type virus, H5ts125, and H5ts149 a second, unique DNA-minus mutant showed that immunologically reactive DNA-binding protein was[r] ... See full document

... Intertypic complementation tests with temperature-sensitive mutants of adenovirus type 5 showed that the mutant in complementation group A failed to complement H5ts125 a DNA-binding prot[r] ... See full document

... DISCUSSION The present investigation describes the isolation of temperature-sensitive mutants of human Ad2, their division into 13 complementation groups, and the construction of a provi[r] ... See full document

... Thus, if the rate of transcription of late The results of experiments reported here help RNA sequences were selectively reduced after to define the role played by the viral DNA the tempe[r] ... See full document

... Little or no infectious virus was produced in cells infected with group I or group III mutants and shifted to 33.5 C in the presence of 5-AC, indicating little or no virion RNA synthesis[r] ... See full document

... 6A, radioactivity was detected in particles having the electron microscopic appearance and the buoyant density of empty capsids p 1.29 g/ml and complete virions p 1.33 g/ml 30, 40.. The [r] ... See full document

... In the infected cells, hexons are produced in vast excess of the amount found in viral particles 643 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Infection of KB cel[r] ... See full document

... It can be seen that ts 3 fails to synthesize any of the capsid antigens; ts 11, ts 4, and ts 7 representing three distinct complementation groups produced normal precipitin lines corresp[r] ... See full document

... protein provides an essential function in the assembly of nascent hexon polypeptide chains into mature hexons, we attempted to develop an in vitro system for the production of hexon in w[r] ... See full document

... The cells were labeled with 200 ,uCi of both [35S]methionine and [3H]thymidine per Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Temperature-sensitive mutants from th[r] ... See full document

... When the RNA species produced during infection by either wild-type virus or tsB9 at the permissive temperature were examined by agarose gel electrophoresis, no difference was seen.. At t[r] ... See full document

... We found that inclusion of Act D at 1 ,u.g/ml three sources in the inoculum during challenge of HeLa cell monolayer cultures inhibited replication of the parent and prototype ts mutant v[r] ... See full document

... Filtration-enrichment and inositol-less death methods of mutant isolation, coupled with a screen for cyanide-insensitive respiration, proved to be highly efficient metho[r] ... See full document

... In tsll6- and ts125 top components and mature virions, no antigenic fiber was detectable, and the SDS polypeptide pattern showed the absence of the fiber polypeptide unit with a molecula[r] ... See full document

... mutants remains the same with increased temperature of incubation, but the plaques get smaller until a temperature is reached at which plaques can no longer be discerned. Th[r] ... See full document

... 20 determined by analytical ultracentrifugation that the molecular weight of the native fiber was approximately 200,000 and that the intact structure consisted of three polypeptide chain[r] ... See full document

... Although FA21 continues after transfer to higher temperature to make DNA at a rate initially identical to the wild type, while FA22 immediately achieves a rate slower than[r] ... See full document