[PDF] Top 20 Sensitive Detection of Multiple Rotavirus Genotypes with a Single Reverse Transcription Real Time Quantitative PCR Assay
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Sensitive Detection of Multiple Rotavirus Genotypes with a Single Reverse Transcription Real Time Quantitative PCR Assay
... the rotavirus VP2 gene were ...of rotavirus can be present together with norovirus in the form of double infections (2), but in order to discard the possibility of cross-reaction with norovirus ... See full document
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Multiplex Real Time Reverse Transcription PCR Assay for Determination of Hepatitis C Virus Genotypes
... genotyping assay was described by Okamoto et al. (24). The assay utilized a first-round amplification of a large section of the core region, followed by a set of second nested amplification reactions each ... See full document
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Development of a Real Time, TaqMan Reverse Transcription PCR Assay for Detection and Differentiation of Lyssavirus Genotypes 1, 5, and 6
... 31). Genotypes 2 to 4 are widely distributed throughout Africa (18), whereas genotypes 5 and 6 are distributed throughout Europe ...A reverse transcription-PCR (RT-PCR) ... See full document
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Highly sensitive detection of the group A Rotavirus using Apolipoprotein H coated ELISA plates compared to quantitative real time PCR
... of rotavirus-associated gastro- enteritis ...specific detection, this method could then be evaluated in future studies for the suitability in a differential diagnosis set-up for the simultaneous ... See full document
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Real Time Reverse Transcription PCR Assay for Detection of Mumps Virus RNA in Clinical Specimens
... the detection of immunoglobulin M-specific antibodies to mumps virus in acute-phase serum samples, the isolation of mumps virus in cell culture, or by detection of the RNA of the mumps virus by ... See full document
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Comparison of Flavivirus Universal Primer Pairs and Development of a Rapid, Highly Sensitive Heminested Reverse Transcription PCR Assay for Detection of Flaviviruses Targeted to a Conserved Region of the NS5 Gene Sequences
... genus PCR procedure could be used as a first-line diagnostic PCR screen- ing test for an unknown virus, indicating the relatedness of the poorly characterized viruses to the pathogenic members of genus ... See full document
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TaqMan Real Time Reverse Transcription PCR Assay for Universal Detection and Quantification of Avian Hepatitis E Virus from Clinical Samples in the Presence of a Heterologous Internal Control RNA
... published real-time PCRs for human and swine HEV (1, 8, 10, 19, 20, 26, 33) have a lower detection limit, but they use DNA as a standard, except for one assay that also used in ... See full document
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Broadly Reactive and Highly Sensitive Assay for Norwalk Like Viruses Based on Real Time Quantitative Reverse Transcription PCR
... 2). Multiple alignment of the ORF1-ORF2 junction region revealed that the most highly conserved nucleotide sequences were nt 5279 to 5381 (102 bases) with respect to Norwalk/68 virus (M87661) among NLV GI and nt ... See full document
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Real Time Reverse Transcription PCR for Detection of Rotavirus and Adenovirus as Causative Agents of Acute Viral Gastroenteritis in Children
... adenovirus genotypes. Examination of the strain diversity of rotavirus A samples was performed using oligonucleotide primers described by DiStefano et ...QIAQuick PCR purification kit and cloned into ... See full document
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Evaluation and Validation of Real Time Reverse Transcription PCR Assay Using the LightCycler System for Detection and Quantitation of Norovirus
... and real-time LC RT-PCR is the absence of a gold ...the real-time LC RT-PCR assay was 4-log more sensitive than a conventional RT-PCR with the same primer ... See full document
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Kinetics of Poliovirus Shedding following Oral Vaccination as Measured by Quantitative Reverse Transcription PCR versus Culture
... Multiplex real-time ...multiplex assay were adopted from Kilpatrick et ...The PCR included the SensiFAST Probe No-ROX One-Step kit (Bioline USA ...of reverse transcriptase, ...CFX96 ... See full document
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Highly Sensitive and Broadly Reactive Quantitative Reverse Transcription PCR Assay for High Throughput Detection of Rift Valley Fever Virus
... highly sensitive and specific molecular diagnostic assay capable of detecting the natural genetic spectrum of RVF ...virus quantitative real-time reverse ... See full document
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Hepatitis E Virus (HEV) Detection and Quantification by a Real Time Reverse Transcription PCR Assay Calibrated to the World Health Organization Standard for HEV RNA
... A real-time reverse transcription-quantitative PCR (RT-qPCR) assay utilizing Pleiades probe chemistry and an RNA internal control for the simultaneous detection and ... See full document
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Sensitive and Specific Quantitative Detection of Rotavirus A by One Step Real Time Reverse Transcription PCR Assay without Antecedent Double Stranded RNA Denaturation
... qRT-PCR assay to use an artificial quan- titative standard that simulates an actual RVA genomic ...NSP3 assay developed in this study has an LOD of approximately 1 seg- ment 7 or NSP3 gene copy per ... See full document
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Real Time Reverse Transcription PCR Assay for Comprehensive Detection of Human Rhinoviruses
... recently, real-time RT-PCR assays have been described for HRVs/HEVs (8, 9, 25, 38, 44) that offer potentially rapid, sensitive, and quantitative results and that are less prone to ... See full document
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Comparison of a Lateral Flow Immunochromatography Assay with Real Time Reverse Transcription PCR for Detection of Human Metapneumovirus
... RNA detection by reverse transcription-PCR (RT-PCR), and antigen detection (2, 3, 4, 5, 6, ...RT- PCR-positive children. Therefore, RT-PCR is concluded to be the ... See full document
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Real time reverse transcription PCR based sequencing independent pathotyping of Eurasian avian influenza A viruses of subtype H7
... A total of 19 samples harbored HP H7 RNA. None of them was detected by the LP specific RT- qPCR (Table 4, Fig. 4). Two isolates originating from the Italian HP H7N1 epizootic of 1999 were detected by the H7 HP ... See full document
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Shedding and Reversion of Oral Polio Vaccine Type 3 in Mexican Vaccinees: Comparison of Mutant Analysis by PCR and Enzyme Cleavage to a Real Time PCR Assay
... the real-time technique include its greater sensitivity and the ease with which the assay is ...that detection of po- liovirus by full-length PCR may have the advantage of better ... See full document
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Single Real Time Reverse Transcription PCR Assay for Detection and Quantification of Genetically Diverse HIV 1, SIVcpz, and SIVgor Strains
... a real-time RT-qPCR assay for detection and quan- tification of viral strains from the HIV-1/SIVcpz/SIVgor ...the time of the previously reported assays in the LTR region, highlighted ... See full document
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A robust HIV-1 viral load detection assay optimized for Indian sub type C specific strains and resource limiting setting
... the assay described in this study specifically designed for Indian Subtype C viruses is useful, economical and reliable for routine monitoring of HIV-1 viral load in resource limited ... See full document
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