[PDF] Top 20 Sensitivity Analysis and Potential Uses of a Novel Gamma Interferon Release Assay for Diagnosis of Tuberculosis

... solution was added to each well, and the plates were incubated at room temperature for a further 30 min. Following the final 30-min incubation, 50 ␮ l of enzyme stopping solution was added to each well and mixed by ... See full document

... evaluating sensitivity and specificity of interferon gamma release assays for the diagnosis of tuberculous pleural effusion using whole-blood (top panel) or pleural fluid (bottom panel) ... See full document

... TB diagnosis in children is hampered by the paucibacillary nature of TB and its extrapulmonary localization and low level of microbiological confirmation (28, ...overall sensitivity and specificity in ... See full document

... pooled sensitivity estimates in culture-confirmed active TB were higher for ...TB, sensitivity was 94% for ...the sensitivity of QFG was 67%, not higher than that of TST in head-to-head comparison ... See full document

... pooled sensitivity of the TST to be 70 to 77% (69% in high- incidence settings and 83% in low-incidence settings) and the sensitivity of QFT-GIT to be 70 to 84% (3, 4, 11, ...cumulative sensitivity ... See full document

... LTBI diagnosis) in the developing countries, such as ...QFT-GIT assay are carried out by machines, more objective, readout results are obtained more quickly than ...easy analysis and management in ... See full document

... to tuberculosis, such as bovine PPD (PPD-b) ...of tuberculosis infection has suggested that there is an appreciable release of IL-10 associated with disease pro- gression, strengthening the case for ... See full document

... the sensitivity of each test is negatively affected by the immunodeficiency of the ...the assay is standardized ...a potential negative effect of low CD4 + T-cell counts on our in-house assay ... See full document

... the sensitivity of T- ...for diagnosis of active pulmonary TB and results have been similar in immunocompetent (83- 100%) and HIV-infected patients (85-93%) ...two potential expla- nations for the ... See full document

... the analysis of PPDb minus PPDa with undiluted stim- ulated ...accurate diagnosis using calculations of PPDb minus ...bovine tuberculosis is hindered by high levels of exposure to NTM that elicit ... See full document

... Debord and colleagues evaluated QFT-G-IT perfor- mance restrospectively in 19 French immunocompetent children (median age: 1.52 years) with active tuberculosis [28]. The rate of indeterminate results was 0/19 and ... See full document

... antemortem diagnosis of tuberculosis (TB) in South American camelids ...The sensitivity and specificity of the gamma interferon (IFN-␥) release assay, two lateral flow ... See full document

... spot” OR CFP10 OR ESAT6) and (tuberculosis[mesh]). No restrictions on date, language, or type of studies were applied. The full search strategy is described in S1 Text. Secondary search- ing of the reference lists ... See full document

... overnight gamma inter- feron (IFN- ␥ ) enzyme-linked immunospot (ELISPOT) assay was performed as previously described ...M. tuberculosis- specific immunodiagnostic assays, as was recently reviewed ... See full document

... ELISPOT assay. For development of an ELISPOT assay to detect specific IFN- ␥ responses against ...M. tuberculosis, recombinant M. tuberculosis antigens and peptides were screened based on the ... See full document

... The transmission of TB in correctional institutions in the United States accounts for approximately 3 to 6% of all TB cases in the country (36). Transmission of TB is problematic in correctional facilities because ... See full document

... Sputum smears were prepared and stained with auramine- phenol[9] and confirmed by Ziehl-Neelson (Z-N). Decon- taminated specimens were inoculated into one slope each of Lowenstein-Jensen medium (L-J) containing glycerol ... See full document

... Our analysis has several ...immunosorbent assay-based IGRA (QFT) and so provides little insight into the performance of enzyme-linked immunospot tests ...the sensitivity data is limited by ... See full document

... = interferon-gamma; ESAT-6 = early secretory antigenic target-6; CFP-10 = culture filtrate protein-10; IU = international units; SFC = spot forming ... See full document

... Each year, about 3,000 Dutch army personnel are deployed to regions where tuberculosis (TB) is highly endemic. Screen- ing of military personnel for latent Mycobacterium tuberculosis infection (LTBI) has ... See full document