[PDF] Top 20 Sequence Based Optimization of a Quantitative Real Time PCR Assay for Detection of Plasmodium ovale and Plasmodium malariae
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Sequence Based Optimization of a Quantitative Real Time PCR Assay for Detection of Plasmodium ovale and Plasmodium malariae
... and PCR assays are the mainstays of malaria diagnostics in resourced settings; how- ever, microscopy remains the gold ...P. malariae and P. ovale based on microscopy present challenges due to ... See full document
6
Detection and identification of human Plasmodium species with real time quantitative nucleic acid sequence based amplification
... is based on microscopic detection of Plasmodium parasites in Giemsa-stained blood ...antigen detection assays and molecular detection assays were introduced as alternatives to ... See full document
6
Real Time PCR for Detection and Identification of Plasmodium spp
... accurate detection of malaria parasites in blood is needed to institute proper ...a real-time PCR assay to detect and distinguish four Plasmodium ...for detection in a ... See full document
6
Evaluation of FRET real time PCR assay for rapid detection and differentiation of Plasmodium species in returning travellers and migrants
... P. ovale infection display mixed infection with real-time PCR ...The detection of P. falciparum plus P. malariae mixed infection was not ...P. malariae with parasitae- mia ... See full document
11
Absence of Plasmodium inui and Plasmodium cynomolgi, but detection of Plasmodium knowlesi and Plasmodium vivax infections in asymptomatic humans in the Betong division of Sarawak, Malaysian Borneo
... all Plasmodium species can cause asymptomatic malaria, there have not been many reports of asymptomatic ...P. malariae [24] and P. ovale [25] infec- tions. Plasmodium malariae infection ... See full document
11
Diagnostic performance of the loop mediated isothermal amplification (LAMP) based illumigene® malaria assay in a non endemic region
... for Plasmodium, accredited according to ...P. ovale, 12 P. malariae) as identified by real-time PCR (Cnops et ...30 Plasmodium negative specimens were ...tested ... See full document
9
Development of a Real Time PCR Assay for Detection of Plasmodium falciparum, Plasmodium vivax, and Plasmodium ovale for Routine Clinical Diagnosis
... Real-time PCR and nested PCR yielded identical ...P. ovale infection in the other tests; one ...P. ovale infection in the other tests; one ...P. ovale infection in the ... See full document
6
Detection of Plasmodium Infection by the illumigene Malaria Assay Compared to Reference Microscopy and Real Time PCR
... is time consuming, labor intensive, and chal- lenging in areas where malaria is not endemic, as it requires continuous proficiency training where positivity rates are ...of quantitative PCR (qPCR) ... See full document
9
Real time PCR detection of Plasmodium directly from whole blood and filter paper samples
... for optimization of the PCR from blood was pur- ified from Plasmodium falciparum 3D7 in vitro culture [9] using DNAzol according to the manufacturer’s pro- tocol (Invitrogen Life Technologies, ... See full document
8
A TaqMan real time PCR assay for the detection and quantitation of Plasmodium knowlesi
... A real-time PCR assay for ...the Plasmodium screening assay published by Rougemont et al for ...P. ovale and P. malariae [30]. Their assay enables rapid ... See full document
7
Real time PCR assay for discrimination of Plasmodium ovale curtisi and Plasmodium ovale wallikeri in the Ivory Coast and in the Comoros Islands
... Plasmodium ovale diagnosis is difficult for several rea- sons, particularly due to a frequent low level of parasit- aemia [8], and the errors of microscopic diagnosis with other malarial ...its ... See full document
8
Classification of Plasmodium Malariae dan Plasmodium Ovale in Microscopic Thin Blood Smear Digital Images
... the Plasmodium parasite transmitted by female Anopheles mosquitoes ...of Plasmodium parasite, which commonly found in Indonesia; they are Plasmodium Malaria (PM), Plasmodium Ovale (PO), ... See full document
7
Epidemiology of Plasmodium infections in Flores Island, Indonesia using real time PCR
... A total of 1,516 blood samples were available for microscopy and PCR. Seven samples were excluded due to failure of the DNA isolation or inhibition of the DNA amplification reaction. Figure 2 shows the flow chart ... See full document
9
Diagnosis of an imported Plasmodium ovale wallikeri infection in Malaysia
... A 19-year-old, Nigerian college student presented to Sunway Medical Centre, Selangor, Malaysia with a 4-day history of fever associated with chills, rigors and body ache. Prior to onset of symptoms, he had gone back to ... See full document
7
A triplex quantitative real time PCR assay for differential detection of human adenovirus serotypes 2, 3 and 7
... traditional PCR method to detect serotypes of adenovirus [25, 26] needs gel electrophor- esis and sequencing, which significantly increases the risk of cross-contamination and is also time consuming and ... See full document
6
A case of Plasmodium malariae recurrence: recrudescence or reinfection?
... Plasmodium malariae is the parasite responsible of quar- tan malaria with the typical periodicity of fever paroxysm observed every 72-h as detailed in a study by Camillo Golgi in 1886, but also described in ... See full document
9
Specificity of the IgG antibody response to Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae, and Plasmodium ovale MSP119 subunit proteins in multiplexed serologic assays
... ity studies. This set included: 3 anonymous, adult blood donor samples collected in 1998 from a region of Haiti with a low prevalence of P. falciparum infection [43]; 9 sera from an integrated serologic study of immune ... See full document
20
A cluster of the first reported Plasmodium ovale spp infections in Peru occuring among returning UN peace keepers, a review of epidemiology, prevention and diagnostic challenges in nonendemic regions
... in the background of mixed infection with P. falciparum, to as low as 2.5% in asymptomatic cross-sectional assess- ments in one 20-year longitudinal study [4]. Concern- ing the risk of P. ovale spp. emerging ... See full document
8
The ears of the African elephant: unexpected high seroprevalence of Plasmodium ovale and Plasmodium malariae in healthy populations in Western Africa
... due to the delayed appearance of these species after the first infection, as has already been observed [23]. Using recombinant proteins from P. falciparum (MSP1 and AMA1), seroprevalence increased after the LRS and ... See full document
8
Recent Advances in Detection of Plasmodium ovale: Implications of Separation into the Two Species Plasmodium ovale wallikeri and Plasmodium ovale curtisi
... the detection of P. ovale and it became evident that P. ovale needed to be divided into classic and variant ...the detection of the variant type of P. ovale and rOVA1 and rOVA2 (NP-1993 ... See full document
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