[PDF] Top 20 Sialic Acid on Herpes Simplex Virus Type 1 Envelope Glycoproteins Is Required for Efficient Infection of Cells

... the cells were incubated with control virions, digested virions, or virions incubated with either Maackia amurensis lectin (MAL1) or Elderberry bark lectin (ELD) (Table 1) at 4°C for 1 ...2,3-linked ... See full document

... latent infection in animal models (8, 43, 68, 74) vali- dated the findings made with these in vitro systems and has led to the suggestion that few, if any, viral gene products are actually required to ... See full document

... mutant virus with respect to the nuclear egress of nucleocapsids is, to some extent, similar to that of wild-type HSV-1(F) infection of nucleolin-depleted cells, nucleolin might ... See full document

... transfected cells, likely via an effect on its phosphorylation (30, 45, ...Vero cells (data not ...HSV-1 infection, ICP27 has been implicated in activating a number of cellular signal ... See full document

... retina infection model ...(Fig. 1). Retinas were stained with polyclonal HSV-1 antibody, which detects many viral pro- teins, including the ectodomains of gB, gC, gD, and gE ...body. Infection ... See full document

... infectious virus in cells or tissues that contain viral genomes, whereas reactivation has been defined as the de novo production of new infectious virus from cells containing latent viral ... See full document

... X-ray crystallography has shown that HVEM binds to a flexible hairpin at the amino terminus of gD [8]. A variety of mutations in this region, including Q27P originally identified in KOS-rid1 virus [22], abolish ... See full document

... UL36GFP-infected cells (Fig. 6D), as previously described for this virus and for UL36 null PrV (9, 16, ...enveloped virus was also seen on the surface of the plasma ...infected cells appeared ... See full document

... a virus that acts to halt cell proliferation would benefit from its ...WT cells. If the absence of growth suppressor p130 is inhibitory to HSV-1 infection and the absence of proliferating ... See full document

... QIF-PC12 cells occurs in the absence of ICP0 but requires ...QIF-PC12 cells, it was important to determine if these gene products were required for spontaneous or stress-induced re- ...detectable ... See full document

... productive infection, at early times after infection, ICP0 transiently localizes to discrete subnuclear structures, known as ND10 or PODs, and as a consequence the cellular ND10 antigens are dispersed (11, ... See full document

... viral infection, cells were submitted to HSV-1 infection 5 days posttransfection with the ...viral infection was determined 24 h ...HeLa cells infected for 8 h with 10 PFU per ... See full document

... the virus? Knowing the amount of glycoprotein present is important for both a struc- tural understanding and to gain insight into possible functional roles for the membrane proteins in ...purified virus of ... See full document

... shPML cells, as no PML protein was detectable by IF ...shGFP cells, MLH1 and PML colocalized; however, in cells expressing shPML, MLH1 was observed in a nuclear diffuse staining pat- tern ...shPML ... See full document

... We have extended these initial studies by measuring the rate of processing at different times after infection and have also determined the rate of appearance of gB and gC at the plasma m[r] ... See full document

... To determine the nature of the linkage of GalNAc added via GalNAc transferase to the immature forms of HSV glycoproteins, we subjected [14C]GaINAc-labeled glycoproteins to a mild alkalin[r] ... See full document

... a virus whose L-S junctions lack a se- quences. This virus still undergoes normal isomerization, indi- cating that neither cleavage and packaging at the a sequence nor nicking of its DR2 repeats is ... See full document

... For long-term lahave examined the polypeptides precipitated by beling of infected cell peptides 1 to 16 h or for shortmonoclonal antibodies secreted by two of the time pulsing 2-h period[r] ... See full document

... In addi- Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest We used monoclonal antibodies reacting with glycoproteins specified by herpes simplex virus type 2 HSV-2 to cha[r] ... See full document

... To examine whether T-cell-depleted effector cells grown in LD culture showed cytotoxicity characteristic of NK cells, LD cultures were also tested against K562 target cells, the cell lin[r] ... See full document