[PDF] Top 20 Structural and Functional Analysis of Hepatitis C Virus Strain JFH1 Polymerase

... of JFH1 and J6 NS5B bound to RNAs corresponding to the 3 ⬘ ends of the genomes of both isolates, but with identical affinities and kinetics for both enzymes and ...the polymerase to ...between JFH1 ... See full document

... or virus morphogenesis (8, 30, 41). However, no functional data have been presented so far to support this ...major structural component of the virion (8). Our functional analysis ... See full document

... The structural implications of this are unclear, but if these residues were involved in disulfide bonds, the putative fusion peptide may be constrained in some ... See full document

... 2a strain, JFH1, was less sensitive to ...(NN/1b/SG), JFH1#4-1 (JFH1/2a/SG), and JFH1#2-3 (JFH1/2a/SG) cells, carrying HCV subgenomic replicon, were treated with 1- ␮ g/ml CsA ... See full document

... the polymerase, which was corroborated by a very closed conformation of the JFH1 thumb favoring the initiation ...of JFH1 with a genotype 2a consensus se- quence (9) and biochemical evidence and was ... See full document

... p3miR-122 tail mutants to rescue replication of the HCV C42G viral RNA. Cell culture experiments were performed using the J6/JFH1 strain and the Renilla luciferase reporter (5). The miR duplexes were ... See full document

... of functional NOD1 ...blot analysis was performed of RIG-I, NOD1, phospho-ERK (p-ERK), and ERK (left ...PCR analysis was performed of IFN- ␤ mRNA (right ... See full document

... ABSTRACT Hepatitis B virus (HBV) encodes a multifunction reverse transcriptase or polymerase (P), which is composed of several ...No structural homologs or high-resolution structure exists for ... See full document

... that polymerase activity as measured by CRC assays is a component of the replication complex and not of isolated ...non- structural proteins per replicase complex would be a more realistic ... See full document

... (B) Analysis of the purification process by Western blotting with anti-PA ...purified polymerase preparation (en) using extracts of virus-infected cells (Flu) as a ...the polymerase ... See full document

... RNA polymerase activity and pHHJFH1/R783A/R785A carrying double Arg-to-Ala substitutions in the cyto- plasmic loop of p7 were constructed by oligonucleotide-directed ...for C-NS2 and C-p7 regions of ... See full document

... that three of the six signature sites (positions 394, 399, and 401) characterizing our T/F viruses were located in HVR1 (Fig. 3). Im- portantly, the signature site located at position 394 was common to the T/F variants ... See full document

... systematic functional analysis of the amino acid residues involved in the low-affinity rGTP- specific binding site of the HCV NS5B, which was previously determined by a crystallographic study ... See full document

... SimPlot analysis are ...HCV strain D10749 with strains AF511949 (sub-type 1A), AY651061 (sub-type 1C) and D45172 (sub-type 2B) is ...shown. Structural and non-structural regions of the genome ... See full document

... tion-PCR analysis of the medium harvested from these cells (data not ...HCV structural pro- teins in the baculovirus expression system (3) or by Semliki Forest virus replicons ...Forest virus ... See full document

... 2a polymerase by X-ray crystallography and to be active inhibitors of the 1b J4 enzyme ...this, structural analysis of the NS5B/thiophene 2 bound complex reveals that the presence of the saturated ... See full document

... A final important observation is the presence of three dis- tinct nucleotide-binding sites in the catalytic center of the enzyme, albeit with the sugars and bases disordered (except for rUTP, as explained above). The ... See full document

... catalytic C-terminal domain of NS2 and the N-terminal protease domain of NS3 (JFH1 polyprotein residues 906–1209, J4 residues 904–1206) flanked by an N-terminal His tag and C-terminal FLAG tag was ... See full document

... The hepatitis C virus (HCV) RNA-dependent RNA polymerase (RdRp), represented by nonstructural protein 5B (NS5B), belongs to a class of integral membrane proteins termed tail-anchored ...the ... See full document

... of hepatitis C virus infected cells reveals that NS5A and NS3 organise into ...JFH-1 strain of hepatitis C virus for 24 h, showed that this protein localised to ... See full document