[PDF] Top 20 Structural and transcriptional analysis of human papillomavirus type 16 sequences in cervical carcinoma cell lines.
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Structural and transcriptional analysis of human papillomavirus type 16 sequences in cervical carcinoma cell lines.
... 962 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest We cloned and analyzed the integrated human papillomavirus type 16 HPV-16 genomes that are present in the human cerv[r] ... See full document
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CpG Methylation of Human Papillomavirus Type 16 DNA in Cervical Cancer Cell Lines and in Clinical Specimens: Genomic Hypomethylation Correlates with Carcinogenic Progression
... genital human papillomaviruses (HPVs) is the primary cause of cervical ...HPV type 16 (HPV-16) DNA is transcriptionally repressed after transfection into cell ...tumor-derived ... See full document
8
The molecular and clinical implications of human papillomavirus-16 mediated oropharyngeal squamous cell carcinoma
... (PMA) analysis was undertaken on a cohort of HPV positive OPSCC (n = 43) and the two HPV16 positive cell lines, CaSki and ...positive cervical cancer cell line ... See full document
258
Transactivation-Competent Bovine Papillomavirus E2 Protein Is Specifically Required for Efficient Repression of Human Papillomavirus Oncogene Expression and for Acute Growth Inhibition of Cervical Carcinoma Cell Lines
... this fashion was not equivalent to the acute growth-inhibitory activity measured here. In fact, the observation that transfec- tion of the E2 gene into HeLa cells can lead to apoptosis (17) raises the possibility that a ... See full document
10
Conserved Methylation Patterns of Human Papillomavirus Type 16 DNA in Asymptomatic Infection and Cervical Neoplasia
... CaSki cell line. In a previous analysis involving one of us (2) of HPV-16 DNA in CaSki cells, amplicons were directly sequenced after bisulfite modification and PCR ampli- fication, and the data ... See full document
11
Rapid, sensitive, type specific PCR detection of the E7 region of human papillomavirus type 16 and 18 from paraffin embedded sections of cervical carcinoma
... HPV 16 and HPV 18 oncogenes E6 and E7 shows a strong positive correlation with the development of invasive cervical carcinoma ...improve type specific detection of HPVs 16 and 18, we ... See full document
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The E7 Oncoprotein Is Translated from Spliced E6*I Transcripts in High-Risk Human Papillomavirus Type 16- or Type 18-Positive Cervical Cancer Cell Lines via Translation Reinitiation
... HPV18-infected cervical cancer cell ...three cell lines, producing mostly E6*I derived from splicing of the nt 226 5 ⬘ ss to the nt 409 3 ⬘ ss for 16E6E7 mRNAs or from splicing of the nt 233 5 ... See full document
15
Analysis of Human Papillomavirus Type 16 (HPV16) DNA Load and Physical State for Identification of HPV16 Infected Women with High Grade Lesions or Cervical Carcinoma
... Integration of the HPV genome in the host cell DNA rep- resents a key step in the progression of lesions, as it allows the continuous overexpression of E6 and E7. Recently, real-time PCR targeting E6 and E2 was ... See full document
8
Association of human papillomavirus type 16 long control region mutation and cervical cancer
... The pGL3 vectors containing LCRs from HPV16 prototype, four samples of HPV16As, one sample of AA1 and one sample of EUR were used for transcriptional analysis. C33A, a human cervical cancer ... See full document
9
Quantitative Measurement of Human Papillomavirus Type 16 E5 Oncoprotein Levels in Epithelial Cell Lines by Mass Spectrometry
... in cervical cancer (18, 64), the HPV-16 genome has been found to persist episomally in 26 to 76% of malignant lesions (9, 11, 16, 22, 37, ...cancer cell lines, SiHa and Caski, harbor 1 ... See full document
9
Human Papillomavirus Type 16 Status in Cervical Carcinoma Cell DNA Assayed by Multiplex PCR
... with human chromo- ...invasive carcinoma) (3, 4, ...E7) sequences may give the infected epithelium growth advantages and may contribute to increased cell proliferation and genomic instability, ... See full document
5
The Global Transcriptional Effects of the Human Papillomavirus E6 Protein in Cervical Carcinoma Cell Lines Are Mediated by the E6AP Ubiquitin Ligase
... HPV type 18 (HPV18)- positive HeLa cervical carcinoma cells and HPV16-positive SiHa and Caski cervical carcinoma cells (American Type Culture Collection) were maintained in ... See full document
11
Structure-function analysis of the human papillomavirus type 16 E7 oncoprotein.
... In this study, a series of site-specific mutations of the human papillomavirus type 16 E7 gene product were constructed and assessed for their effects on intracellular protein stability,[r] ... See full document
10
Identification of the FHL2 Transcriptional Coactivator as a New Functional Target of the E7 Oncoprotein of Human Papillomavirus Type 16
... E7. Cell lysates were prepared and subjected to immunoprecipitation (IP) with antibodies to E7 (goat anti-HPV-16 E7; Amynon, Innsbruck, Austria) and Flag (Sigma, Vienna, Austria) and control antibodies, as ... See full document
6
A point mutational analysis of human papillomavirus type 16 E7 protein.
... It is evident from these studies that mutants which destroy both transforming and trans-activation activities p24GLY and mutants which are transformation competent but express no or very[r] ... See full document
7
Gene Expression Profiles are Altered in Human Papillomavirus 16 E6 D25E Expressing Cell Lines
... both cell lines, the expression of E6 proteins was observed at similar ...structed cell lines based on the mRNA level of the downstream-regulated survivin ... See full document
6
Integration of human papillomavirus 16 in esophageal carcinoma samples
... In the present study, HPV DNA from 189 patient tis- sue samples with pathologic diagnosis of esophageal car- cinoma were examined, and a high prevalence was found (approximately 89% HPV DNA positive rate), and the HPV16 ... See full document
9
Repression of endogenous p53 transactivation function in HeLa cervical carcinoma cells by human papillomavirus type 16 E6, human mdm-2, and mutant p53.
... 6 Repression of Endogenous p53 Transactivation Function in HeLa Cervical Carcinoma Cells by Human Papillomavirus Type 16 E6, Human mdm-2, and Mutant p53 FELIX HOPPE-SEYLER* AND KARIN BUT[r] ... See full document
7
Cervical Human Papillomavirus (HPV) Infection and HPV Type 16 Antibodies in South African Women
... and cervical cancer ...of cervical cancer for age, ethnic group, and residential ...or cervical cancer ...squamous carcinoma or adenocarcinoma of the cervix (n ⫽ 524) were identified at two ... See full document
8
Variable Oncogene Promoter Activity of Human Papillomavirus Type 16 Cervical Cancer Isolates from Australia
... man cervical cancer sample (10, 15) using luciferase activity as reporter of E6 and E7 ...of cervical cancer- derived cells (HeLa [HPV18 positive] and HT3 [HPV nega- tive]) by calcium phosphate ... See full document
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