[PDF] Top 20 Structure of a bacterial type III secretion system in contact with a host membrane in situ
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Structure of a bacterial type III secretion system in contact with a host membrane in situ
... containing membrane fragments in the culture medium, which can trigger type III secretion in chlamydial elementary bodies 42 ...the host-free state revealed a similar distribution of ... See full document
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Making connections: snapshots of chlamydial type III secretion systems in contact with host membranes
... with host membranes, which act as critical interfaces between pathogen and ...with host membranes for the first time in any infection system [26 ,41 ...the host membrane and other ... See full document
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Structure of a bacterial type IV secretion core complex at subnanometre resolution
... a contact-dependent manner (Dreiseikelmann, ...T4S system (Terradot and Waksman, 2011); the plasmid pTi from Agrobacterium tumefaciens encodes the VirB/D T4S system, which is able to deliver an ... See full document
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The Ruler Protein EscP of the Enteropathogenic Escherichia coli Type III Secretion System Is Involved in Calcium Sensing and Secretion Hierarchy Regulation by Interacting with the Gatekeeper Protein SepL
... the host cells is efficient and timely, the secretion process is tightly regulated and consists of three groups of secreted substrates (summarized in reference ...the secretion apparatus (“early” ... See full document
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Membrane and Core Periplasmic Agrobacterium tumefaciens Virulence Type IV Secretion System Components Localize to Multiple Sites around the Bacterial Perimeter during Lateral Attachment to Plant Cells
... to host cells through multiprotein type IV secretion system (T4SS) complexes that span the bacterial cell envelope is critical to bacterial ...lateral contact with plant ... See full document
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Role of Autocleavage in the Function of a Type III Secretion Specificity Switch Protein in Salmonella enterica Serovar Typhimurium
... ABSTRACT Type III secretion systems (T3SSs) are multiprotein machines employed by many Gram-negative bacteria to inject bacterial effector proteins into eukaryotic host cells to promote ... See full document
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Self Labeling Enzyme Tags for Analyses of Translocation of Type III Secretion System Effector Proteins
... in host cells, SPI1-T3SS and Yersinia effector proteins are preformed and delivered upon contact to the host ...translocator structure and the mode of loading of effector proteins into the ... See full document
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The Structure of an Injectisome Export Gate Demonstrates Conservation of Architecture in the Core Export Gate between Flagellar and Virulence Type III Secretion Systems
... irulence-associated type III secretion systems (T3SS), also termed injectisomes, are bacterial nanomachines that facilitate the delivery of effector proteins directly into a eukaryotic ... See full document
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Mutations in the Borrelia burgdorferi Flagellar Type III Secretion System Genes fliH and fliI Profoundly Affect Spirochete Flagellar Assembly, Morphology, Motility, Structure, and Cell Division
... overall structure and protein composition similar to those found in other bacteria ...collar structure associated with the rotor, (ii) multiple homol- ogous flagellar filament proteins (FlaB) in Treponema, ... See full document
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Structure of HrcQ(B)-C, a conserved component of the bacterial type III secretion systems
... total membrane fraction by ultracentrifugation at 65,000 ⫻ g for 40 ...total membrane fraction was mixed with urea or NaCl or Triton X-114 and incubated on ice for 30 min before ultracentrifugation at ... See full document
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A Phosphorylation Switch on Lon Protease Regulates Bacterial Type III Secretion System in Host
... ring structure with an internal sequestered degradation chamber for proteolysis (21, ...many bacterial pathogens by degrading key regulatory proteins (16–18, 23, 24, ... See full document
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The Biochemistry of Sensing: Enteric Pathogens Regulate Type III Secretion in Response to Environmental and Host Cues
... Bile salts are a component of biliary secretions that are released into the small intestine to solubilize fats. They also have antimicrobial properties against bacteria that inhabit or transit the gastrointestinal tract ... See full document
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Migration of Type III Secretion System Transcriptional Regulators Links Gene Expression to Secretion
... purifier system (Amersham) and a prepacked Hi-Prep 16/60 Sephacryl S-200 high-resolution column (GE ...(HPLC) system (Shimadzu) operating with the LCsolution software and equipped with a solvent delivery ... See full document
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Engineering the flagellar type III secretion system: improving capacity for secretion of recombinant protein.
... the secretion construct incorporated FLAG tag ...luorimetric secretion assay, based on cleavage of 4-methylumbelliferyl butyrate (MUB) by cutinase to yield a luorescent 4-methylumbelliferone (4-MU) ...of ... See full document
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Engineering the flagellar type III secretion system: improving capacity for secretion of recombinant protein
... improved secretion in the absence of the 3 ′ UTR, as FlgM might be more readily secreted from the FT3SS—how- ever at this time we have no evidence to support this the- ...direct secretion and ensure ... See full document
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Topological Analysis of the Type 3 Secretion System Translocon Pore Protein IpaC following Its Native Delivery to the Plasma Membrane during Infection
... of membrane permeabilization, PEG5000-maleimide was added in the presence of ...the membrane fraction, and the pellet consisted of the detergent-insoluble fraction, which included intact ... See full document
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A Review on Virulence Factors of Escherichia Coli
... outer membrane protein A, the use of type III secretion systems by the bacteria to subvert eukaryotic signaling pathways by injecting virulence proteins into the host cell cytoplasm, ... See full document
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EspZ of Enteropathogenic and Enterohemorrhagic Escherichia coli Regulates Type III Secretion System Protein Translocation
... T3SS effectors function in a coordinated manner. As effectors from a single pathogen can complement or antagonize each other, the timing and location of their activity, as well as their quantity, must be highly ... See full document
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Overexpression of serine protease HtrA enhances disruption of adherens junctions, paracellular transmigration and type IV secretion of CagA by Helicobacter pylori
... the secretion of the protease by ~1.8-fold. Interestingly, the secretion of other well-known bacterial virulence determinants, VacA and GGT, was not affected by HtrA overexpression, suggesting that ... See full document
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Mycosins Are Required for the Stabilization of the ESX 1 and ESX 5 Type VII Secretion Membrane Complexes
... nitrocellulose membrane, and membranes were stained with anti-GroEL2 (Cs44; John Belisle, NIH, Bethesda, MD, USA), anti- PE_PGRS (31), anti-ESAT-6 (monoclonal antibody [MAb] Hyb76-8), anti-HA ... See full document
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