[PDF] Top 20 Structure and Function of the N-Terminal Domain of the Vesicular Stomatitis Virus RNA Polymerase
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Structure and Function of the N-Terminal Domain of the Vesicular Stomatitis Virus RNA Polymerase
... Negative-strand RNA viruses include a diverse set of viral families that infect animals and plants, causing serious illness and eco- nomic ...viral polymerase, which performs a unique set of reactions to ... See full document
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Optimal Replication Activity of Vesicular Stomatitis Virus RNA Polymerase Requires Phosphorylation of a Residue(s) at Carboxy-Terminal Domain II of Its Accessory Subunit, Phosphoprotein P
... the RNA-dependent RNA polymerase of the ...viral polymerase, its interaction with the large protein, L, generates a specific P-L complex(es) which is absolutely essential for the ... See full document
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Crystal Structure of the Oligomerization Domain of the Phosphoprotein of Vesicular Stomatitis Virus
... the N-RNA template while the L polymerase reads the ...one N monomer at a time, dictating that the polymerase complex moves 6 nucle- otides each ...its RNA ... See full document
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Putative Domain-Domain Interactions in the Vesicular Stomatitis Virus L Polymerase Protein Appendage Region
... L polymerase protein similarly organized into multiple domains that work in concert to enable virus genome transcription and ...for domain-domain interactions and domain contiguity in ... See full document
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Access to RNA Encapsidated in the Nucleocapsid of Vesicular Stomatitis Virus
... the N proteins of VSV and RABV, there has been speculation about how the N pro- tein might undergo a conformational change in order to expose the encapsidated ...The RNA encapsidation cavity is lo- ... See full document
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Role of the intergenic dinucleotide in vesicular stomatitis virus RNA transcription.
... The function of the gene junction is to modulate polymerase activity such that during transcription, the flanking genes are expressed as mRNA in quantities that render the virus most fit in the pop- ... See full document
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Mutations in the C-Terminal Loop of the Nucleocapsid Protein Affect Vesicular Stomatitis Virus RNA Replication and Transcription Differentially
... 2.9-Å structure of the vesicular stomatitis virus nucleocapsid (N) protein bound to RNA shows the RNA to be tightly sequestered between the two lobes of the N ... See full document
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Phosphorylation within the amino-terminal acidic domain I of the phosphoprotein of vesicular stomatitis virus is required for transcription but not for replication.
... Taken together, results shown in Fig. 5 suggest that increasing amounts of the mutant P proteins (P 60/64 and P 60/62/64 ) do not rescue their in vivo transcription activities. Replication and transcription supported by ... See full document
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High resolution structure of the n terminal endonuclease domain of the lassa virus L polymerase in complex with magnesium ions
... crystal structure of the N-terminal region of the LCMV L protein (PDB: 3JSB) [24] reveals an endonuclease domain that is structurally homologous to the endonuclease domain of the ... See full document
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Importance of Hydrogen Bond Contacts between the N Protein and RNA Genome of Vesicular Stomatitis Virus in Encapsidation and RNA Synthesis
... in structure among members of the Rhab- doviridae (16, ...the N protein and RNA might not only stabi- lize the RNP structure but also play a critical role in the function of the RNP ... See full document
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Structural Properties of the C Terminus of Vesicular Stomatitis Virus N Protein Dictate N-RNA Complex Assembly, Encapsidation, and RNA Synthesis
... containing N gene mutations display defects in ...in N oligomerization and N-P-RNA complex as- sembly associated with individual C-terminal ...for N EF419/420AA , were able to ... See full document
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Role of Intermolecular Interactions of Vesicular Stomatitis Virus Nucleoprotein in RNA Encapsidation
... the N oligomerization completely but certainly subverted the ability of the N protein to encapsidate ...the N-lobe seems to be more flexible than the C-lobe, as shown by the different orientations of ... See full document
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Late Domain Function Identified in the Vesicular Stomatitis Virus M Protein by Use of Rhabdovirus-Retrovirus Chimeras
... L domain of Rous sarcoma virus (RSV), an avian ...in vesicular stomatitis virus (VSV) M protein has an activity analogous to that of the retroviral sequence, M-Gag chimeras were ...The ... See full document
7
Terminal sequences of vesicular stomatitis virus RNA are both complementary and conserved.
... A comparative study of nucleotide sequences at the 3' termini of ribonucleic acids from vesicular stomatitis virus and its defective interfering particles, p. Todaro (ed.),[r] ... See full document
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Transcriptional activity and mutational analysis of recombinant vesicular stomatitis virus RNA polymerase.
... However, we devel- oped a simpler protocol using crude cytoplasmic extracts (33) from these transfected cells that will efficiently support VSV transcription in vitro in conjunction with[r] ... See full document
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Analysis of in vitro transcription products of intracellular vesicular stomatitis virus RNA polymerase.
... The RNA transcripts synthesized in vitro by the intracellular transcriptase complex of VSV- infected cells are copied from virtually all re- gions of the infectious virus genome at both [r] ... See full document
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In vivo transcription of the 5'-terminal extracistronic region of vesicular stomatitis virus RNA.
... In vivo transcription and polyadenylation at the junction of the L cistron and the 5'-terminal extracistronic region of vesicular stomatitis virus RNA was inves-.. tigated.[r] ... See full document
7
Vesicular stomatitis virus RNA polymerase can read through the boundary between the leader and N genes in vitro.
... Triphosphorylated in vitro transcripts of vesicular stomatitis virus were selected.. by mercury-Sepharose chromatography using adenosine-5'-O-(3-thiotriphos-.[r] ... See full document
5
Structure and origin of a snapback defective interfering particle RNA of vesicular stomatitis virus.
... Specific modification of the G residue in position 1' followed by complete RNase Ti digestion gave rise to a new oligonucleotide which spanned the region from position 33' on the minus s[r] ... See full document
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Vesicular Stomatitis Virus mRNA and Inhibition of Translation of Cellular mRNA—Is There a P Function in Vesicular Stomatitis Virus?
... In general, the size of 'hough not shown polysomes translating any given cellular mRNA actin synthesis at was proportional to the size of the protein prod-.. n-s used[r] ... See full document
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