[PDF] Top 20 Structure of Hepatitis C Virus Polymerase in Complex with Primer-Template RNA
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Structure of Hepatitis C Virus Polymerase in Complex with Primer-Template RNA
... the hepatitis C viral (HCV) genome is accomplished by the NS5B RNA-dependent RNA polymerase (RdRp), for which mechanistic understanding and structure-guided drug design efforts ... See full document
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Template Requirements for RNA Synthesis by a Recombinant Hepatitis C Virus RNA-Dependent RNA Polymerase
... an RNA with 3⬘-deoxyadenosine (cordy- cepin, which possesses a 2⬘-hydroxyl) retained the ability to di- rect RNA ...of RNA synthesis in vitro has now been demonstrated by a num- ber of groups ... See full document
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Structural Analysis of the Hepatitis C Virus RNA Polymerase in Complex with Ribonucleotides
... the hepatitis C virus (HCV) RNA polymerase with ribonucleoside triphosphates (rNTPs) and divalent metal ...each complex with nucleo- ...initiation complex reported for the ... See full document
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Selection of 3′-Template Bases and Initiating Nucleotides by Hepatitis C Virus NS5B RNA-Dependent RNA Polymerase
... novo RNA synthesis by hepatitis C virus (HCV) nonstructural protein 5B (NS5B) RNA-dependent RNA polymerase has been investigated using short RNA ...novo RNA ... See full document
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Template/Primer Requirements and Single Nucleotide Incorporation by Hepatitis C Virus Nonstructural Protein 5B Polymerase
... quaternary complex. In this report, specific template/primer requirements for efficient RNA synthesis by HCV NS5B were ...copy-back RNA synthesis, NS5B utilizes templates with an ... See full document
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Evidence for activation of the hepatitis B virus polymerase by binding of its RNA template.
... Protease digestion profiles of in vitro synthesized DHBV P. The preceding data suggest that an interaction between TYDP and ε during or shortly after translation enables the enzyme to perform the trans assay. An ... See full document
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Template Requirements for De Novo RNA Synthesis by Hepatitis C Virus Nonstructural Protein 5B Polymerase on the Viral X RNA
... X RNA, which direct the transcription initiation in the pyrimidine-rich region, it was observed that the reaction efficiency was maximized at the Xdel (3) ...X RNA, it could be suggested that the positions ... See full document
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Membrane Association of the RNA-Dependent RNA Polymerase Is Essential for Hepatitis C Virus RNA Replication
... The hepatitis C virus (HCV) RNA-dependent RNA polymerase (RdRp), represented by nonstructural protein 5B (NS5B), belongs to a class of integral membrane proteins termed ... See full document
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Oligomerization and Cooperative RNA Synthesis Activity of Hepatitis C Virus RNA-Dependent RNA Polymerase
... NS5B RNA-dependent RNA polymerase encoded by hepatitis C virus (HCV) plays a key role in viral ...NS5B polymerase acts as a functional ...the C-terminal hydrophobic ... See full document
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De Novo Initiation of RNA Synthesis by the RNA-Dependent RNA Polymerase (NS5B) of Hepatitis C Virus
... The RNA products used in the above RNase protection assay were first treated with RNase T1 to remove excess input RNA ...complementary RNA product to the input template was converted from the ... See full document
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The Hepatitis C Virus RNA-Dependent RNA Polymerase Membrane Insertion Sequence Is a Transmembrane Segment
... the C terminus of NS5B is translo- cated to the ER lumen also in the context of a functional HCV replication complex, the NS5B-gt construct was introduced into a subgenomic ...The C terminus of NS5B ... See full document
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Characterization of Soluble Hepatitis C Virus RNA-Dependent RNA Polymerase Expressed in Escherichia coli
... a template- primer complex and a ribonucleotide are ...the template-primer first to form a binary complex which then takes up a nucleotide to form the catalytically competent ... See full document
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Sphingomyelin Activates Hepatitis C Virus RNA Polymerase in a Genotype-Specific Manner
... for Virus Research, Kyoto University, Kyoto 606-8507, Japan 5 ; and Chiba Institute of Technology, 2-17-1 Tsudamuna, Narashino, Chiba 275-0016, Japan 6 Received 25 March 2010/Accepted 27 August 2010 ... See full document
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De Novo Synthesis of Negative-Strand RNA by Dengue Virus RNA-Dependent RNA Polymerase In Vitro: Nucleotide, Primer, and Template Parameters
... dengue virus RNA-dependent RNA polymerase and a subgenomic 770-nucleotide RNA template, it was shown previously that the ratio of the de novo synthesis product to hairpin product ... See full document
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Selection of RNA Aptamers That Are Specific and High-Affinity Ligands of the Hepatitis C Virus RNA-Dependent RNA Polymerase
... small RNA molecules that are specific and high-affinity ligands of nonstructural 5B (NS5B) polymerase, we screened by SELEX (systematic evolution of ligands by exponential amplification) a structur- ally ... See full document
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A Recombinant Hepatitis C Virus RNA-Dependent RNA Polymerase Capable of Copying the Full-Length Viral RNA
... homopolymeric RNA templates only in the presence of a ...HCV RNA, which is conserved in all genotypes of HCV, as a template, a distinct RNA product was ...98-nt RNA abolished almost ... See full document
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Specific Interaction between the Hepatitis C Virus NS5B RNA Polymerase and the 3′ End of the Viral RNA
... positive-sense RNA viruses may provide some ...genomic RNA occurs, and replication of the viral RNA begins with the synthesis of mi- nus-strand RNA which then serves as the template for ... See full document
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Mutagenesis Analysis of the rGTP-Specific Binding Site of Hepatitis C Virus RNA-Dependent RNA Polymerase
... HCV RNA replication be? The exact reason for the reduction of HCV RNA replication in the cell by mutations of the low-affinity rGTP-binging site of the HCV RdRp is not ...HCV RNA replication ... See full document
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Identification of a C-Terminal Regulatory Motif in Hepatitis C Virus RNA-Dependent RNA Polymerase: Structural and Biochemical Analysis
... the C-terminal region, comprising residues 545 to 564, interfered with RNA binding and consequently affected the observed rate of product forma- tion as shown in several experiments ...enhanced ... See full document
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Regulation of De Novo-Initiated RNA Synthesis in Hepatitis C Virus RNA-Dependent RNA Polymerase by Intermolecular Interactions
... The hepatitis C virus (HCV) RNA-dependent RNA polymerase (RdRp) has been proposed to change conformations in association with RNA synthesis and to interact with cellular ... See full document
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