[PDF] Top 20 Structure of the murine leukemia virus envelope glycoprotein precursor.

... DISCUSSION The data presented demonstrate that the Moloney MuLV glycoprotein precursor Pr8Oenv is a molecule with a apoprotein portion having a molecular weight of 60,000 and at least tw[r] ... See full document

... the structure shown in ...surface. Virus produced from these cells lacked TM protein, failed to bind to receptor, and had no detectable ...chimeric envelope was re- ported to be viable ...infectious ... See full document

... amphotropic envelope and 44 amino acids long with two in- ternal disulfide bridges in the ecotropic ...MLV envelope-receptor interaction are located in segment 1 of VRA (referred to as region B of the ... See full document

... the precursor envelope protein into the mature SU and TM subunits is essential for retroviral ...uncleaved precursor protein into virions, the re- sulting particles are noninfectious (references 8, ... See full document

... influenza virus HA influence fusion ...crystal structure of HA at neutral pH, His17 forms part of a surface of ionizable residues that interact with the fusion peptide at pH ...amphotropic envelope ... See full document

... The rat cell Une XC, which undergoes cell-to-cell fusion upon infection with ecotropic MuLV, was transfected with wild-type env expression vectors, and high levels of syncytium formation[r] ... See full document

... Moloney murine leukemia virus (MoMLV) and related or heterologous viruses produces progeny pseudotype virions bearing the MoMLV genome encapsulated by the envelope of the other ... See full document

... Since glycoGag has been described to be dispensable ex vivo, we investigated the in vivo effects of a glycoGag- mutation in the Friend murine leukemia virus F-MuLV.. F-MuLV induces sever[r] ... See full document

... To test whether these viral protein defects were the result of an altered viral genome or due to defective cellular p30' processes, clone 7C was reinfected with Friend MuLV, and the prog[r] ... See full document

... of envelope expression vector and trun- cated SU ...MuLV envelope glycoprotein (GP) and of truncated envelope fragments (SU, PRO, and ST), showing VRA and VRB (black boxes), PRO (hatched ... See full document

... MoMuLV envelope protein in fusion and subsequent retroviral transduc- tion, a series of truncations, deletions, and amino acid substi- tutions were constructed in this ...mutant envelope proteins were ... See full document

... surface glycoprotein gp120 nor the unprocessed Env precursor was detected in the particulate fraction when truncated HIV-1 Env was expressed alone or when wild-type HIV-1 Env was coex- pressed with Mo-MLV ... See full document

... foamy virus (FV) subgroup of retroid viruses has at- tracted scientific interest because of their unique replication strategy and because of their potential use as gene transfer vectors ...this virus group, ... See full document

... The functional importance of residues adjacent to the pock- et in the large loop (loop 1) formed by the disulfide bond be- tween C73 and C83 (Fig. 1) was also examined. Not surprising- ly, if either one of these cysteine ... See full document

... generally due to failure to achieve a compact globular confor- mation (8, 19). Also consistent with defective folding, elevated levels of fragments of SU were present in the supernatant medium for most of the gs4 region ... See full document

... Cotransfection of Envs at a 1:1 DNA ratio (the amount of each Env plasmid being half that when transfected alone) led in most cases to increased total and cell surface Env (Fig. 2A and C, lanes 7 to 10). We infer that ... See full document

... To determine the positions of pl5env and pl2env within the precursor protein, the amino acid sequence of the F-MuLV env polypeptide was compared with the partial amino acid sequences det[r] ... See full document

... Recently, we demonstrated that leucine insertions in the MSD of MLV Env have a significant effect on glycoprotein functionality (35). Insertion of one (⫹1L) or two (⫹2L) leucines in the MSD abrogated function, ... See full document

... lymphotropic virus type 1 (HTLV-1) is the agent of adult T-cell leukemia (ATL) (37) and HTLV-1-asso- ciated myelopathy or tropical spastic paraparesis (HAM/TSP) ...the virus, proviral load, viral ... See full document

... unprocessed precursor molecules as a predomi- nant species because of a deficiency in SU-TM proteolytic processing and processed Env as a very minor species retain part of their infectious potential, indicating ... See full document