[PDF] Top 20 Structure of the 3' terminus of the hepatitis C virus genome.

... Hepatitis C virus (HCV), a positive-strand RNA virus, has been considered to have a poly(U) stretch at the 3 * terminus of the ...HCV genome by primer extension analysis ... See full document

... (type virus, yellow fever virus) and the pestiviruses ...diarrhea virus) ...the genome RNA is ; ...HCV genome RNA contains a short 3 9 NTR followed by a poly(U) homopolymer tract ... See full document

... RNA virus, the 3 9 end of the viral genome is the initiation site for the synthesis of negative-strand RNA, an intermediate product of RNA ...The 3 9 -end region of genomic RNA often forms ... See full document

... In an effort to identify an etiologic agent of posttransfusion non-A, non-B hepatitis, we have recently cloned overlapping cDNAs that span the entire coding region of HCV isolated from t[r] ... See full document

... the 3 9 NTR commences with a short sequence ( ; 28 to 42 nt) that is poorly conserved among different genotypes followed by a poly(U)/polypyrimidine tract of variable length and ...authentic 3 9 ... See full document

... The 3⬘ end of the HCV (⫺) strand RNA is the strict anti- sense sequence of the (⫹) strand ...IRES structure, respectively, as well as for the nucleotides forming the external loop of SL-BI, which are also ... See full document

... NS5B C-terminal coding re- ...60-amino-acid C-terminal domain of NS5B, which is known to be dispensable for enzymatic activity in vitro ...secondary structure of the remaining portion of NS5BCR (data ... See full document

... In the case of non-replicating mutations, it was important to establish that this elimination of replication resulted from a loss or disruption of a specific function of NS5A, rather than disruption of polyprotein ... See full document

... generated 3⬘ and 5⬘ products in the correct molecular size range, consistent with ...processive 3⬘-to-5⬘ exonucle- ...negative-strand 3⬘ ...The 3⬘ product of digestion can vary in length ... See full document

... The 3 * end of the simian hemorrhagic fever virus (SHFV) single-stranded RNA genome was cloned and ...the 3 * poly(A) tract, we identified a 76-nucleotide noncoding region preceded by two ... See full document

... RNA structure still allowed expression of a functional NS5B, because the insertion of the intact version of ...of hepatitis B virus, reverse transcription is primed by the addition of the first 4 nt ... See full document

... Gorbalenya and Koonin have proposed that helicases be classified into three superfamilies (SF1, SF2, and SF3) and two (smaller) families (6). SF1 and SF2 are the two largest super- families of helicase proteins that ... See full document

... the virus interacts with its receptor represents an important factor in determining both the onset and the progression of viral infection, the first objective of this work was to determine the affinity of the ... See full document

... Preparation of partial dsRNA substrates. The partial double-stranded RNA (dsRNA) substrates were prepared by transcribing in vitro a portion of the multiple cloning sequences of a pGEM vector (Promega) in both ... See full document

... wild-type structure, allowing for strictly conserved residue Gly410 to come within ...a 3=-deoxy terminal resi- due, the other carboxylate oxygen of Asp225 is free to hydrogen bond with ...polio- ... See full document

... reference genome. A few bases at the end of NS5B lacked sufficient coverage in 3 of 17 samples, generating ...the 3= region due to RNA secondary struc- ...in 3= UTR affecting the proximal NS5B ... See full document

... chimeric genome, we conducted trans complementation ...HCV genome with the amino acid position at the N terminus of each HCV protein ...and 3 ⬘ maximum overlapping regions among the defective ... See full document

... RNA extraction, reverse transcription-PCR, cloning, and sequencing. Viral RNA was extracted from both serum (140-␮l) and liver (0.05-g) samples using QIAamp viral RNA binding columns (Qiagen). Isolated HCV RNA was ... See full document

... miRNA-122 binding-sites are not essential for genotype 2 RNA replication, which is in contrast to what was found with genotype 1 replicons (10, 11). Replication of mutants SL-IIz ⬘ - 56-59, SL-IIz ⬘ -78-82, and SL-IIz ⬘ ... See full document

... the 3⬘-UTR of transferrin receptor (TfR) mRNA (23, 64) and functions to coordinate posttranscriptional regulation of cellu- lar iron metabolism (17, ...the 3⬘-UTR of TfR mRNA stabilizes this transcript and ... See full document