[PDF] Top 20 A study of the in vitro initiation of adenovirus DNA replication

... human adenovirus serotypes probably reflects a crucial functional constraint on this domain, which makes it a very likely candidate for containing the sequences which are critical for recognition by ...a ... See full document

... RB69 DNA Pol has been deter- mined in the absence of substrates and displays a very simi- lar arrangement of conserved residues at the active site ...RB69 DNA Pol, ␾ 29 DNA Pol, human DNA Pol ... See full document

... short replication products characteristic of displacement of the elongating strand from the ...that replication of short substrate mol- ecules in extracts from uninfected cells gave almost as much ... See full document

... cell DNA synthesis is blocked during the Epstein-Barr virus (EBV) lytic infection cycle, during which there is high- level amplification of the EBV genome ...cellular DNA synthesis in lytic infected cells ... See full document

... ribosomal DNA origin recognition via complementary base pairing ...of replication are achieved also need further elucida- ...recent study using a novel hybrid CHO cell/ Xenopus egg extracts, a pre- ... See full document

... Using an in vitro system in which purified fractions or crude extracts of adenovirus type 4-infected HeLa cells can support initiation and elongation on linearized plasmid templates cont[r] ... See full document

... In addition to conserved sequences at the origin of DNA replication, a protein has been found covalently attached to the 5' termini of adenovirus DNA and has a molecular weight of 139 Do[r] ... See full document

... stranded DNA (ssDNA), double-stranded DNA (dsDNA) and RNA in vitro ( reviewed in Chase and Williams, 1986 ; Stuiver, 1993) with at least a 5-fold preference for ssDNA over dsDNA; binding of ICP8 to ... See full document

... DNA replication is a highly regulated cellular process in proliferating cells, involving cell cycle dependent assembly of DNA replication-initiation proteins (DRIPs) onto origins of ... See full document

... during DNA replication (14, 40, 43). This DNA replication-cou- pled system of histone deposition is thought to also be utilized by some DNA ...the DNA replication of ... See full document

... In a recent paper that described the cloning of AdPol 30a, DNA sequences contained within the exon at genome coordinate 39 were shown to be essential for expression of active AdPol prote[r] ... See full document

... The formation of 80-kd protein-nucleotide complexes is completely dependent upon the presence of adenovirus DNA containing the attached 55-kd terminal protein; no detectable 80-kd protei[r] ... See full document

... in vitro, Rep-dependent initiation of replication has been demonstrated to occur even in the absence of a hairpin structure (20, 26, ...new replication fork ...B19V DNA 4244 is the ... See full document

... T This report describes linker insertion mutants with muta- within the pTP ORF which were inserted into a transient expression system and assayed for their ability to direct protein tran[r] ... See full document

... We demonstrate that virion-derived DNA-protein complexes from five human adenovirus serological subgroups A to E can act as a template for both the initiation and the elongation of DNA r[r] ... See full document

... Microarray analysis. MCF10A cells were serum starved for 36 h fol- lowed by infection with the indicated viruses for 16 h, and RNA was isolated using a RNAeasy total RNA isolation kit (Qiagen). The RNA purification ... See full document

... Since SV40 DNA replication absolutely depends on SV40 T antigen, anti-T serum from a tumor-bearing hamster was added to the reaction mixture containing both HeLa nuclear extract and CosI[r] ... See full document

... in vitro approaches have been used to demonstrate that Drosophila ORC binds to replication elements that direct initiation in the chorion gene cluster (Figure 1/6, Austin et a l , ...the DNA ... See full document

... DiaA is a positive regulator of DNA replication initiation in E. coli influencing the frequency and timing of the initiation event [56]. It functions by binding to domain I of DnaA and ... See full document

... Subsequent analysis of the strand distribution of pulse-label in the double-stranded, terminal restriction enzyme fragments from these molecules in the course of either early displacemen[r] ... See full document