[PDF] Top 20 SUMO 1 possesses DNA binding activity
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SUMO 1 possesses DNA binding activity
... SUMO-1 DNA complexes can also be detected using UV-crosslinking ...GST-SUMO-1 DNA complexes specific to the presence of a 65 bp double-stranded mismatch-free DNA ... See full document
7
Evaluation of Interactions of Human Cytomegalovirus Immediate-Early IE2 Regulatory Protein with Small Ubiquitin-Like Modifiers and Their Conjugation Enzyme Ubc9
... exogenous SUMO-1 in a transient-cotransfection assay for enhancement of cyclin E ex- ...other SUMO-1 substrates such as p53, PML, and RanGAP1, we propose two possible mechanisms for these ... See full document
14
Transformation by the fos or jun oncogene does not increase AP-1 DNA-binding activity.
... To determine whether transformation by the v-fos or v-jun oncogene results in alterations in the level or regulation of this factor, we have characterized AP-1 DNA-binding activity in nu[r] ... See full document
9
Upregulation of TRAF 3 by shear stress blocks CD40 mediated endothelial activation
... Although inhibition of TRAF-3 only partially reversed the protective effect of shear stress, TRAF-3 overexpres- sion prevented CD40L-induced MCP-1 expression, sug- gesting that TRAF-3 is sufficient to block CD40 ... See full document
9
Noncovalent SUMO interaction motifs in HIV integrase play important roles in SUMOylation, cofactor binding, and virus replication
... SIM/SUMO binding vary and are largely dependent on SUMOylated proteins and SIM-containing binding ...partners. SUMO binding through SIM(s) affects protein stability, cytosolic-nuclear ... See full document
14
Temperature regulates hypoxia inducible factor 1 (HIF 1) in a poikilothermic vertebrate, crucian carp (Carassius carassius)
... on DNA binding of HIF-1 dimer (Richard et ...HIF-1 activity appears also to be needed for maintenance of energetic homeostasis in tissues at normal physiological oxygen ... See full document
10
Induction of AP-1 DNA-binding activity and c-fos mRNA by the adenovirus 243R E1A protein and cyclic AMP requires domains necessary for transformation.
... These data strongly suggest that specific cellular ElA-binding proteins are involved in the induction of AP-1 DNA-binding activity and c-fos mRNA by the synergistic action of the 243R EI[r] ... See full document
11
Microwave synthesis, spectral characterization DNA binding studies and biological activity of Cr(III), Co(II), Ni(II), Hg(II) and Cd(II) complexes with nicotinic acid hydrazide and nitrite mixed ligand complexes
... stranded DNA in different binding modes on the basis of their structure, charge and type of ...As DNA double helix possesses many hydrogen bonding sites which are accessible both in the minor ... See full document
5
DNA Binding Activity of the Herpes Simplex Virus Type 1 Origin Binding Protein, UL9, Can Be Modulated by Sequences in the N Terminus: Correlation between Transdominance and DNA Binding
... double-stranded DNA containing the box I sequence of HSV-1 ...strong DNA binding ability with slightly reduced efficiency compared to the C ter- ...the DNA binding assay as a ... See full document
10
Generation of specific inhibitors of SUMO-1– and SUMO-2/3–mediated protein-protein interactions using Affimer (Adhiron) technology
... with SUMO 1, SUMO 2, ubiquitin, bovine serum albumin (BSA), or no ...with SUMO 1 and SUMO ...proteins. DNA sequencing revealed that loop 2 of S1B1 was ...loop 1 ... See full document
40
Moldovan, George-Lucian (2006): Regulation of replication-linked functions by PCNA and SUMO. Dissertation, LMU München: Fakultät für Biologie
... PIP-box binding to the hydrophobic cleft of PCNA can be dramatically altered by even modest changes in the identity of the non-conserved aminoacids in the QxxL/I/MxxFF/Y box, as well as of those N- or C-terminal ... See full document
123
Extracellular cyclophilin A possesses chemotaxic activity in cattle
... with DNA ligation kit ver. 2.1 (Takara). After DNA sequencing, the plasmid was used to transform BL21 (DE3) (Bio-Rad), and the cells were treated with ...enzymatic activity to cleave between the ... See full document
10
Cytosolic chaperonin CCT possesses GTPase activity
... In the present study, we established a convenient purifi- cation method of CCT. This method uses ATP-Sepharose as a purification step and purified protein had a ring-like complex with a molecular mass of approximately ... See full document
8
Human Pathogens and the Host Cell SUMOylation System
... and SUMO-modified PML is conserved within the Alphaherpesviri- nae, allowing them to target PML-NBs for dispersal, whereas the RING domain has variable functions, which nevertheless might include degradation of ... See full document
13
Regulation of Sp2 DNA-Binding Activity and trans-Activation
... bind DNA, COS-1 cells were transiently transfected with an EYFP-Sp2 expression vector, non-denatured nuclear extracts were prepared, and these and control extracts were subsequently incubated with a ... See full document
198
TRIM5α is a SUMO substrate
... binding conditions, which together may point to the fact that SUMOylation is transitory and affects only a small proportion of a given protein. Since rhTRIM5α K10R mutant is still able to restrict HIV-1, ... See full document
6
Thermolabile in vivo DNA-binding activity associated with a protein encoded by mutants of herpes simplex virus type 1.
... 3 Thermolabile In Vivo DNA-Binding Activity Associated with a Protein Encoded by Mutants of Herpes Simplex Virus Type 1 Received 30 December 1982/Accepted 17 March 1983 The major DNA-bin[r] ... See full document
11
SUMO Binding by the Epstein-Barr Virus Protein Kinase BGLF4 Is Crucial for BGLF4 Function
... kinase activity of BGLF4 played a role in abolishing ZTA SUMOylation, ZTA was cotransfected with the kinase-dead BGLF4 ...kinase activity also abolished the ability of BGLF4 to inter- fere with ZTA ... See full document
10
Role of SUMO 1 and SUMO interacting motifs in rhesus TRIM5α mediated restriction
... restriction activity in these cell lines (Figure 1B, ...antiviral activity against both N-MLV and ...the binding of rhTRIM5α to the HIV-1 capsid ... See full document
11
Baculovirus Alkaline Nuclease Possesses a 5′→3′ Exonuclease Activity and Associates with the DNA-Binding Protein LEF-3
... the bound enzyme with buffers containing KCl concentrations up to 1 M, followed by elution with 150 mM imidazole. Anal- ysis of the protein fractions collected from this resin by SDS– 10% PAGE followed by the ... See full document
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