[PDF] Top 20 Template-Dependent Initiation of Sindbis Virus RNA Replication In Vitro
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Template-Dependent Initiation of Sindbis Virus RNA Replication In Vitro
... complete replication of RNA could result because the prepa- ration lacks a cofactor necessary for plus-strand ...strand RNA with high affinity (24, 25), and it has been postu- lated that this protein ... See full document
8
Influenza A virus RNA-dependent RNA polymerase: analysis of RNA synthesis in vitro.
... to replication are not understood, although several hy- potheses have been postulated to account for the multiple enzymatic functions of the influenza virus polymerase ...new virus-encoded proteins ... See full document
9
De Novo Initiation of RNA Synthesis by the Arterivirus RNA-Dependent RNA Polymerase
... initiated RNA synthesis de novo on homopolymeric templates in a template-specific ...poly(A) template. This is consistent with the observation that de novo RNA synthesis generally initiates ... See full document
12
Residues of the Rotavirus RNA-Dependent RNA Polymerase Template Entry Tunnel That Mediate RNA Recognition and Genome Replication
... the RNA backbone, perhaps this bond is broken and reformed with the next most 5 ⬘ residue, as a ⫹ RNA template moves through the ...the template RNA molecule, which would be ... See full document
12
Selection of 3′-Template Bases and Initiating Nucleotides by Hepatitis C Virus NS5B RNA-Dependent RNA Polymerase
... of RNA templates af- fects the activity. In vitro, two key interactions may affect the de novo polymerase activity: (i) interaction between the RNA template and polymerase and (ii) interaction ... See full document
10
RNA-RNA recombination in Sindbis virus: roles of the 3' conserved motif, poly(A) tail, and nonviral sequences of template RNAs in polymerase recognition and template switching.
... genomic RNA? Some plus-stranded RNA viruses appear to deliver polymerase proteins to the 39 end of the genome by coupling genome translation and replication (38, ...supports replication of DI ... See full document
12
Given the Opportunity, the Sendai Virus RNA-Dependent RNA Polymerase Could as Well Enter Its Template Internally
... the replication promoters depended on the position of the nucleotides vis-a`-vis the six N protein interaction sites ...efficient replication from the external promoter, the internal promoter was not used ... See full document
9
De Novo Initiation of RNA Synthesis by the RNA-Dependent RNA Polymerase (NS5B) of Hepatitis C Virus
... the virus (6, 50, ...in vitro studies using various recombinant systems (6, ...the initiation of translation of the viral polyprotein (6, 12, 13, ...for replication of the viral RNA ... See full document
13
RNA Replication without RNA-Dependent RNA Polymerase: Surprises from Hepatitis Delta Virus
... the replication of HDV RNA, similar to that of plant viroids, is mediated by cellular poly- ...HDV replication, it was first suggested that pol II carries out HDV RNA replication based ... See full document
8
Biochemical and Genetic Studies of the Initiation of Human Rhinovirus 2 RNA Replication: Purification and Enzymatic Analysis of the RNA-Dependent RNA Polymerase 3Dpol
... an RNA polymerase activity was first detected in membrane-bound complexes of HRV2- infected HeLa cells ...be template and primer dependent in vitro ...viral RNA sequences were used as ... See full document
10
Alfalfa Mosaic Virus Coat Protein Bridges Rna and Rna-dependent Rna Polymerase in Vitro
... strand RNA synthesis (Degraaff, Tveld, and Jaspars, 1995; Houwing and Jaspars, 1986; Olsthoorn et ...facilitates replication by organizing the 3' conformation for replicase binding and accurate ...In ... See full document
25
Gamma Interferon-Dependent, Noncytolytic Clearance of Sindbis Virus Infection from Neurons In Vitro
... inhibit virus replication and that de novo cellular protein synthesis might be required for complete inhibition of SINV ...phavirus replication is zinc finger antiviral protein (ZAP) ...genomic ... See full document
12
Complete replication in vitro of tobacco mosaic virus RNA by a template-dependent, membrane-bound RNA polymerase.
... membrane-bound RNA polymerase preparations from tomato plants infected with TMV-L, we were able to detect only the genomic-length ...the RNA polymerase synthe- sized genomic-length ssRNA as well as the ... See full document
8
Minimal Template Requirements for Initiation of Minus-Strand Synthesis In Vitro by the RNA-Dependent RNA Polymerase of Turnip Yellow Mosaic Virus
... were detected, indicating that a completely double- stranded product of 43 bp is obtained, taking into account that the 39 A residue is not transcribed (see above) and removed by nuclease S1. Similar results are ... See full document
8
Host Factors Associated with the Sindbis Virus RNA-Dependent RNA Polymerase: Role for G3BP1 and G3BP2 in Virus Replication
... enhanced replication, first detected at 2 h after infection, was maintained for the remainder of the infection (P ⬍ ...SINV replication as a result of silencing the G3BPs would result in a change in ... See full document
13
Efficient Interaction between Arenavirus Nucleoprotein (NP) and RNA-Dependent RNA Polymerase (L) Is Mediated by the Virus Nucleocapsid (NP-RNA) Template
... MG RNA at 4°C for 16 h, prior to PD with Strep-Tactin resin (Strep-Tactin Super flow Plus, Qiagen) at 4°C for 2 ...of RNA transcript (500 ng) used for panel A was examined by agarose gel ... See full document
5
Template Role of Double-Stranded RNA in Tombusvirus Replication
... plus-strand RNA [( ⫹ )RNA] viruses is a rela- tively simple process driven by the viral replicase complex ...to replication, and the ( ⫹ )RNAs become templates to produce the complementary ... See full document
14
Coding Sequences Enhance Internal Initiation of Translation by Hepatitis A Virus RNA In Vitro
... A virus (HAV) belongs to the Picornaviridae fam- ily and is the sole member of the Hepatovirus ...positive-strand RNA of about ...a virus-encoded proteinase into various structural and nonstructural ... See full document
7
The fate of RNA and RNA binding proteins in Sindbis virus infection
... chikungunya virus and Venezuelan equine encephalitis virus (Carrasco, ...SINV RNA genome was first sequenced in ...positive-sense RNA molecule generated from an internal promoter, that harbors ... See full document
111
Mechanism of De Novo Initiation by the Hepatitis C Virus RNA-Dependent RNA Polymerase: Role of Divalent Metals
... (nt) RNA, LE19 ...the initiation position, LE19 allows us to use GTP only for initia- tion and not for ...of RNA synthesis by all three recombinant ...novo initiation, primer extension, ... See full document
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