[PDF] Top 20 The 5′-Terminal Region of the Aichi Virus Genome Encodes cis-Acting Replication Elements Required for Positive- and Negative-Strand RNA Synthesis
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The 5′-Terminal Region of the Aichi Virus Genome Encodes cis-Acting Replication Elements Required for Positive- and Negative-Strand RNA Synthesis
... loop region (nt 55 to 60) of SL-B of mut112-118 to restore the base-pair interaction with nt 112 to 118, excluding the guanosine at nt 116 (mut55-60/112-118) ...the RNA replication and plaque-form- ... See full document
14
Structural and Functional Analysis of the cis-Acting Elements Required for Plus-Strand RNA Synthesis of Bamboo Mosaic Virus
... for virus movement and encapsidation, respectively ...block” required for viral movement, as demonstrated with White clo- ver mosaic virus ...ORF 5 encodes the 25-kDa coat ... See full document
8
The 5′ and 3′ Downstream AUG Region Elements Are Required for Mosquito-Borne Flavivirus RNA Replication
... 11-kb-long RNA ge- nome of positive polarity (see reference ...viral RNA encodes one open reading frame (ORF), and the resulting polyprotein is cleaved co- and posttranslationally into 3 ... See full document
6
Poly(A) at the 3′ End of Positive-Strand RNA and VPg-Linked Poly(U) at the 5′ End of Negative-Strand RNA Are Reciprocal Templates during Replication of Poliovirus RNA
... Many positive-strand RNA viruses ...their RNA genomes (16, 25), yet the mechanisms by which 3 ⬘ poly(A) sequences are derived during viral replication are un- ...Picornavirus RNA ... See full document
16
Cis-acting Elements Important for Potato Virus X Minus-strand RNA Synthesis In Vitro and In Vivo
... two replication mechanisms. Poliovirus replication protein 3CD and EF-1α in the RdRp complex are required for the activity (Harris et ...bridge 5’ and 3’ termini of the poliovirus ... See full document
184
The 5′ Terminal Trailer Region of Vesicular Stomatitis Virus Contains a Position-Dependent cis-Acting Signal for Assembly of RNA into Infectious Particles
... on RNA rep- lication was quantitated following a separate analysis of immu- noprecipitated RNAs (Table ...replicated RNA revealed that as the deletions re- duced the 59 terminus below 45 nt, the level of ... See full document
9
3′-Terminal Sequence in Poliovirus Negative-Strand Templates Is the Primary cis-Acting Element Required for VPgpUpU-Primed Positive-Strand Initiation
... the 5 ⬘ clover- leaf were also shown to be required to maintain viral RNA stability (13, ...the 5 ⬘ cloverleaf, was required to maintain the stability of viral RNA in the HeLa ... See full document
13
In Vitro- and In Vivo-Generated Defective RNAs of Satellite Panicum Mosaic Virus Define cis-Acting RNA Elements Required for Replication and Movement
... necrosis virus (STNV), satellite panicum mosaic virus (SPMV), satellite maize white line mosaic virus, and satellite tobacco mosaic virus (STMV) (4, ...of RNA molecules that are ... See full document
8
Stem-Loop IV in the 5′ Untranslated Region Is a cis-Acting Element in Bovine Coronavirus Defective Interfering RNA Replication
... (nt) 5 ⴕ untranslated region (UTR) in the positive-strand bovine coronavirus (BCoV) genome is predicted to contain four higher-order structures identified as stem-loops I to IV, which ... See full document
13
Interaction between Polypeptide 3ABC and the 5′-Terminal Structural Elements of the Genome of Aichi Virus: Implication for Negative-Strand RNA Synthesis
... the 5 ⬘ -terminal region of the genome, Northwest- ern analysis was performed using various mutant RNA probes which contain a single or double mutation to disrupt or restore the base ... See full document
11
Poliovirus 5′-Terminal Cloverleaf RNA Is Required in cis for VPg Uridylylation and the Initiation of Negative-Strand RNA Synthesis
... Proximal RNA termini: a common strategy for RNA animal viruses? RNA animal viruses appear to utilize communication between the 5⬘ and 3⬘ termini of their viral RNAs as a common strategy to ... See full document
13
Mutagenic Analysis of the 3′ cis-Acting Elements of the Rubella Virus Genome
... rubella virus (RUB) genome, a region conserved in all RUB defective interfering RNAs, revealed four stem-loop (SL) structures; SL1 and SL2 are both located in the E1 coding region, while SL3 ... See full document
18
Uncoupling cis-Acting RNA Elements from Coding Sequences Revealed a Requirement of the N-Terminal Region of Dengue Virus Capsid Protein in Virus Particle Formation
... Nt2 virus propa- gated slowly, cells were passed every 4 days and were analyzed by ...Nt1 virus continued to increase, and about 60% of the monolayer was in- fected at day ...viral RNA was purified ... See full document
13
Interplay of RNA Elements in the Dengue Virus 5′ and 3′ Ends Required for Viral RNA Replication
... of RNA replication of replicon RNAs harboring the 3 ⬘ NoDAR mutation was due to interference with HP-3 ⬘ SL formation, we introduced additional mutations into the 3 ⬘ sequence to restore the formation of ... See full document
16
Requirements for Assembly of Poliovirus Replication Complexes and Negative-Strand RNA Synthesis
... (PV) RNA replication have contributed to new insights into this complex ...distinct elements on the viral RNA (vRNA) in various steps leading to production of new vRNA ...template-dependent ... See full document
10
Sequences in the 5′ Nontranslated Region of Hepatitis C Virus Required for RNA Replication
... HCV 5⬘ NTR were fused via the 63-nucleotide spacer to the IRES of PV were used to program HeLa cell extracts as described in Materials and ...HCV 5⬘ NTR is shown in lane 3 (rep5.1). Note that this ... See full document
11
A cis-acting mutation in the Sindbis virus junction region which affects subgenomic RNA synthesis.
... In this study, by using a full-length cDNA clone of the Sindbis virus genome from which infectious transcripts can be generated by in vitro transcription 29, we report the construction o[r] ... See full document
12
cis-acting requirements for the replication of flock house virus RNA 2.
... The 5' ends of RNA 2 transcripts expressed from different plasmids were mapped by primer extension and compared with the 5' ends of their positive-strand replication products and authent[r] ... See full document
8
Kissing of the two predominant hairpin loops in the coxsackie B virus 3' untranslated region is the essential structural feature of the origin of replication required for negative-strand RNA synthesis.
... The enteroviruses can be divided into two main groups ac- cording to serological tests and genetic analysis, one subgroup containing the polioviruses and most coxsackie A viruses and the other consisting of coxsackie B ... See full document
11
Capsid Protein Synthesis from Replicating RNA Directs Specific Packaging of the Genome of a Multipartite, Positive-Strand RNA Virus
... RNA1 replication, Sf21 cells were infected with each of the AcR1 deletion mutants described above, and total cellular RNA was extracted 3 days after ...the RNA samples demonstrated that, relative to ... See full document
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