[PDF] Top 20 Transcriptome analysis of mouse stem cells and early embryos.
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Transcriptome analysis of mouse stem cells and early embryos.
... of stem cells. Transcriptome analysis of these pluripotent cells is a first step towards such ...adult stem cells, we obtained 249,200 high- quality EST sequences and ... See full document
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Single cell RNA seq transcriptome analysis of linear and circular RNAs in mouse preimplantation embryos
... expression levels than those with repeat element complementary sequences in their adjacent flanking introns (Figs. 4b; Fig. S8c in Additional file 1). Interestingly, the complementary se- quences in the adjacent ... See full document
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The tissue specific transcriptomic landscape of the mid gestational mouse embryo
... The mouse is a well-established model organism for the investigation of mammalian development and human ...the mouse genome sequence has provided access to any gene locus in the ...function analysis ... See full document
6
Transcriptome analysis of mouse and human sinoatrial node cells reveals a conserved genetic program
... pacemaker cells, which is essential for the generation of biological pacemakers, relies on functional studies in mice and cell ...the transcriptome of human SANs will improve the robustness of pacemaker ... See full document
15
Identification and functional analysis of long non-coding RNAs in human and mouse early embryos based on single-cell transcriptome data
... comprehensive analysis of human and mouse early-stage embryonic lncRNAs was performed based on public single-cell RNA sequencing ...profile analysis revealed that lncRNAs are expressed in a ... See full document
14
GDF3, a BMP inhibitor, regulates cell fate in stem cells and early embryos
... P19 cells, mouse GDF3 reduced BMP4 ...frog embryos and in mouse pluripotent ...frog embryos, we examined cell fate changes induced in response to GDF3 in three independent contexts: ... See full document
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A developmental framework for induced pluripotency
... somatic cells, but not in pluripotent cells and promotes terminal differentiation during development (Ambros, ...the mouse embryo, let-7 expression starts at embryonic day (E) ...in mouse and ... See full document
12
Isolation and propagation of enteric neural crest progenitor cells from mouse embryonic stem cells and embryos
... crest cells in the presence of noggin, Wnt3a, Lif and ...crest cells from E10.5 S10G chimaera. (B) qRT-PCR analysis of marker mRNA expression in: S10G-S ES cells; Sox10-GFP-positive ... See full document
12
Gene expression profiles during early differentiation of mouse embryonic stem cells
... Bioinformatic analysis of confirmed genes (part ...the mouse chromosome to which the EST ...ES cells versus compartments of early embryos (GSE8881), and/or similar published ... See full document
18
Human Stem Cell Research: scientific uncertainties and ethical dilemmas
... Embryonic stem cells (ES cells) These can be derived from 5-6-day old human embryos, which have been obtained by in vitro ...20-100 cells located inside the vesicle, the inner cell ... See full document
8
Haploid genomes illustrate epigenetic constraints and gene dosage effects in mammals
... haploid cells from early mouse embryos Following studies on haploid mammalian embryos, ini- tial attempts to derive pluripotent ES cells from haploid mouse blastocysts ... See full document
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MicroRNAs control the apoptotic threshold in primed Pluripotent stem cells through regulation of BIM
... 200 embryos at 5.5 dpc, 100 embryos at 6.5 dpc, 20 embryos at ...10 embryos at ...in embryos, total RNA samples were processed and analyzed by Geneservice using the TaqMan mouse ... See full document
7
Analysis of parent specific gene expression in early mouse embryos and embryonic stem cells using high resolution two dimensional electrophoresis of proteins
... Int, I, D,,', Bioi, ~O ~99 506 (1996) 499 Oligillal Article Analysis of parent specific gene expression in early mouse embryos and embryonic stem cells using high resolution two dimensional electropho[.] ... See full document
8
Genome wide gene expression analysis in mouse embryonic stem cells
... the cells exhibited the properties of stem cells ...that cells were capable of long-term ...whether cells could be subcultured after freez- ing, thawing and ...MES3 cells were ... See full document
13
SETDB1 in Early Embryos and Embryonic Stem Cells
... Immunofluorescence revealed that after fertilization, SETDB1 gradually appears in the pronucleus, with the stronger signal in the male pronucleus than the female pronucleus (Cho et al., 2012) (Fig. 3). It leads to a ... See full document
11
Comparative transcriptome analysis of embryonic and adult stem cells with extended and limited differentiation capacity
... from mouse MSCs, mClone-3, mMAPC-1 and -2, and ESCs were double amplified and labeled with the Two-Cycle Target Labeling and Control Reagents kit P/N 900494 (Affymetrix, Santa Clara, CA) following the ... See full document
20
Human Stem Cell Research: scientific uncertainties and ethical dilemmas
... Embryonic stem cells (ES cells) These can be derived from 5-6-day old human embryos, which have been obtained by in vitro ...20-100 cells located inside the vesicle, the inner cell ... See full document
8
Comparative study of mouse and human feeder cells for human embryonic stem cells
... fibroblast-like cells autologously derived from hESCs (Bendall et ...a transcriptome analysis of feeder cells revealed that significant differences exist between supportive and non-supportive ... See full document
12
Decoding breast cancer tissue–stroma interactions using species specific sequencing
... co-cultured cells, the genome-wide transcriptional profiles were quite ...juxtaposed cells in co-culture are methods frequently used to activate Notch signaling (for example in breast cancer research) it ... See full document
14
Effect of substrate stiffness on early human embryonic stem cell differentiation
... on stem cell differentiation was provided by qualitative studies involving mouse mammary epithelial cells that showed increased differentiation when grown on soft gel colla- gen substrates as opposed ... See full document
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