[PDF] Top 20 Trypsinization dependent cell labeling with fluorescent nanoparticles
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Trypsinization dependent cell labeling with fluorescent nanoparticles
... of fluorescent NPs into living cells are carried out on proliferating cells, suggesting that they have been trypsinized for a short time before the NP ...the cell plasma mem- brane. Indeed, cell ... See full document
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Application of functional quantum dot nanoparticles as fluorescence probes in cell labeling and tumor diagnostic imaging
... Pang et al. have also made a number of breakthroughs in cell labeling and imaging research. For example, as an intracellular indicator, CdSe QD fluorescence was success- fully used in genetic techniques, ... See full document
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FMN-Coated Fluorescent USPIO for Cell Labeling and Non-Invasive MR Imaging in Tissue Engineering
... the labeling of collagen-based scaffold materials with ultrasmall superparamagnetic iron oxide nanoparticles (USPIO) enables sensitive and longitudinal monitoring of their location, func- tion and ... See full document
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Original Article Cytotoxicity of CdSe quantum dots and corresponding comparison with FITC in cell imaging efficiency
... emerging fluorescent nanoparticles, quantum dots applied in cellular imaging has elic- ited broad research ...high fluorescent quantum yield, broad absorp- tion and narrow emission spectrum, the ... See full document
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Fabrication of Positively Charged Fluorescent Polymer Nanoparticles for Cell Imaging and Gene Delivery
... functionalized nanoparticles could break up the vesicle membrane via a proton sponge effect ...is labeling a positively charged polymer molecules (poly acrylic acid, PAA) with red dyes (Alexa 594-NHS) and ... See full document
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Stem Cell Labeling using Polyethylenimine Conjugated (α-NaYbF4:Tm3+)/CaF2 Upconversion Nanoparticles
... for cell labeling due to its high sensitivity, cost-effectiveness, and rapid processing ...typical labeling agents such as organic dyes and fluorescent nanoparticles emit visible light ... See full document
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Activity-Based Protein Profiling of Alkane-Oxidizing Monooxygenases in Rhodococcus rhodochrous ATCC 21198.
... CuAAC-dependent labeling using AlexaFluor 647 azide produced detectable fluorescence ...that fluorescent label remains attached to specific polypeptides after heating in SDS-PAGE sample buffer ... See full document
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Feedback tracking and correlation spectroscopy of fluorescent nanoparticles and biomolecules
... living cell, in which a there is not only a random short-duration change in the diffusion coefficient during transport, but also a change in the topology of the space over which the molecule is ... See full document
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Paclitaxel-loaded iron platinum stealth immunomicelles are potent MRI imaging agents that prevent prostate cancer growth in a PSMA-dependent manner
... magnetic nanoparticles were collected (purified away from unbound antibody) using a magnetic ...green fluorescent paclitaxel (Invitrogen) prior to ... See full document
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Commercial Nanoparticles for Stem Cell Labeling and Tracking
... more, fluorescent silica NPs are presently the only type of fluorescent NP approved for human clinical ...stem cell labeling and tracking in clinical trials (940 clinical trials involving stem ... See full document
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Silver Nanoparticles As Fluorescent Probes: New Approach For Bioimaging
... for Nanoparticles synthesis but chemical reduction method is frequently used and characterized using NMR, XRD and ...FT-IR. Fluorescent molecules enable scientists to detect particular subunit of ... See full document
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Deconstructing the genesis of animal form
... a cell- autonomous lineage label to follow the fate of hair follicle placode cells that once expressed ...progenitor cell fate in more than one organ ... See full document
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Magnetic Enrichment of Dendritic Cell Vaccine in Lymph Node with Fluorescent-Magnetic Nanoparticles Enhanced Cancer Immunotherapy
... promote DC entry into the lymphatic vessels. Unlike previous studies that relied on chemokine gradients to guide DC migration, we showed that α-AP-fmNP-loaded DCs were sensitive to MPF, and their migration efficiency ... See full document
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Cell type-dependent uptake, localization, and cytotoxicity of 1.9 nm gold nanoparticles
... gold atoms per cell indicate comparative uptake potentials, irrespective of size. Furthermore, the hyperconcentration of intracellular gold demonstrates active uptake of the particles to concentrations many times ... See full document
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Autoantibody profiling for the study and treatment of autoimmune disease
... from patients with autoimmune disease. They provide a powerful tool to characterize autoreactive B-cell responses in diseases including rheumatoid arthritis, multiple sclerosis, autoimmune diabetes, and systemic ... See full document
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Study on performance of magnetic fluorescent nanoparticles as gene carrier and location in pig kidney cells
... gold nanoparticles (NPs) are reported to provide the highest imaging resolution in fixed cells due to their visibility under a transmission electron microscope [13], but they do not allow real-time imaging of live ... See full document
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Fluorescent pegylated nanoparticles demonstrate fluid phase pinocytosis by macrophages in mouse atherosclerotic lesions
... dot nanoparticles by fluid-phase ...plasma-treated fluorescent quantum dots (x axis) for 5 hours, and then macrophage uptake of fluorescent nanoparticles (y axis) was determined as described ... See full document
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The migration of synthetic magnetic nanoparticle labeled dendritic cells into lymph nodes with optical imaging
... centrations of SPIO (0, 10, 25, 50 µ g/mL) were added to the cell medium. After incubation of 12 hours, the medium was removed, the cells were washed and cytospins were prepared. Thereafter, the amount of ... See full document
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Superparamagnetic Iron Oxide Nanoparticles as MRI contrast agents for Non-invasive Stem Cell Labeling and Tracking
... and cell lines [63, ...for cell uptake ...charged cell mem- ...and labeling efficiency in rat MSCs ...stem cell internal- ization due to the specific surface chemistry, despite they ... See full document
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ClearT: a detergent and solvent free clearing method for neuronal and non neuronal tissue
... Anterograde DiI labeling has been described previously (Plump et al., 2002). The eye was placed back into the optic cup and heads were incubated in PBS containing 0.1% sodium azide as follows: E14-E16, 5-7 days at ... See full document
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