[PDF] Top 20 Usefulness of matrix-assisted laser desorption ionization time-of-flight mass spectrometry to identify pathogens, including polymicrobial samples, directly from blood culture broths
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Usefulness of matrix-assisted laser desorption ionization time-of-flight mass spectrometry to identify pathogens, including polymicrobial samples, directly from blood culture broths
... derived from monomicrobial and polymicrobial BC ...in polymicrobial cases, analysis of corrected mixture ratios of combinations of the candidate infecting bacteria may facilitate detection of indi- ... See full document
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Real Time Identification of Bacteria and Candida Species in Positive Blood Culture Broths by Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry
... in blood culture bottles, but none of these reach the level of performance of ...fluid from a positive blood culture into an automated identification system ...identification ... See full document
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Evaluation of blood culture broths with lysis buffer to directly identify specific pathogens by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry methods
... The mass spectra show the difference in peaks (presence or absence) and their intensities between the sample spectrum and those of bacteria identified at first pass by ...bioMérieux blood culture ... See full document
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Direct Identification of 80 Percent of Bacteria from Blood Culture Bottles by Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Using a 10 Minute Extraction Protocol
... ABSTRACT Matrix-assisted laser desorption ionization–time of flight mass spectrom- etry is not widely used to identify bacteria directly from positive ... See full document
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Performance of Two Resin Containing Blood Culture Media in Detection of Bloodstream Infections and in Direct Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry (MALDI TOF MS) Broth Assays for Isolate Identification: Clinical Comparison of the BacT/Alert Plus and Bactec Plus Systems
... (2–4). Blood culture (BC) remains the gold standard for diagnosing BSIs (5, ...in culture media and the availability of software-assisted, automated growth detectors have enhanced the recovery ... See full document
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Identification of Rare Pathogenic Bacteria in a Clinical Microbiology Laboratory: Impact of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
... identification directly from colonies and samples us- ing matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) mass ... See full document
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Rapid Identification of Bacteria and Yeasts from Positive Blood Culture Bottles by Using a Lysis Filtration Method and Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrum Analysis with the SARAMIS Database
... infections directly from a positive blood culture would decrease the time to directed antimicrobial therapy and greatly improve patient ...care. Matrix-assisted ... See full document
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Direct Identification of Urinary Tract Pathogens from Urine Samples by Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry
... To identify unknown bacteria, each peak list generated was matched directly against reference librar- ies (3,290 species) using the integrated pattern-matching algorithm of the Bio- typer ... See full document
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Direct Identification of Urinary Tract Pathogens from Urine Samples, Combining Urine Screening Methods and Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
... microorganisms from direct urine samples in order to provide early diagnosis of UTIs; performing this methodology on the day of sample collection reduces the identification of the etiological agent by 18 to ... See full document
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Comparison of Two Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry Methods with Conventional Phenotypic Identification for Routine Identification of Bacteria to the Species Level
... to identify multiple organisms at once in a single assay, with sufficient sensitivity and specificity to improve on culture-based strate- gies and in a shorter time ...clinical samples is ... See full document
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Utility of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry following Introduction for Routine Laboratory Bacterial Identification
... to identify bacteria directly from positive blood culture broths when colonial morphology is not immediately available ... See full document
5
Advances in Identification of Clinical Yeast Isolates by Use of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
... hands-on time (29, ...to identify yeast isolates directly from positive blood culture broths demonstrated an average savings of $1,837 per patient based on early ... See full document
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Identification of Blood Culture Isolates Directly from Positive Blood Cultures by Use of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and a Commercial Extraction System: Analysis of Performance, Cost, and Turnaround Time
... monomicrobial blood cul- ture isolates tested. This compares favorably with data from others using in-house extraction systems for blood culture analysis which have reported correct ... See full document
5
Comparison of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and Molecular Biology Techniques for Identification of Culicoides (Diptera: Ceratopogonidae) Biting Midges in Senegal
... MALDI-TOF MS analysis. (i) Creating a reference database of biomarker masses. MALDI-TOF MS reference spectra were determined for 437 field-caught Culicoides biting midge species from Senegal, including C. ... See full document
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Detection of Polyclonality among Clinical Isolates from Prosthetic Joint Infections
... Our results support the role of RAPD typing as a useful tool for the detection of genomic polymorphisms (28). In fact, there have been studies of the strain differentiation of S. aureus based on this technique (16, 29). ... See full document
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Comparing Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and Phenotypic and Molecular Methods for Identification of Species within the Streptococcus anginosus Group
... Given that the S. constellatus subsp. pharyngis is found on a cluster branching from within S. constellatus subsp. constellatus (Fig. 1), this was not unexpected. In fact, a similar problem is found with the ... See full document
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Implementation of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry in Routine Clinical Laboratories Improves Identification of Coagulase Negative Staphylococci and Reveals the Pathogenic Role of Staphylococcus lugdunensis
... originated from blood ...MS. From a clinical standpoint, it should be em- phasized that this pathogen was described in several pathological instances, especially in immunocompromised patients, ... See full document
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Creation of an In House Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Corynebacterineae Database Overcomes Difficulties in Identification of Nocardia farcinica Clinical Isolates
... ranged from 52 to 59 carbons, with the main peak belonging to mycolic acid, with a chain length of 55 carbon atoms and 4 double bonds (the observed value of the sodi- ated molecular ion was m/z ... See full document
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Identification of clinical dermatophyte isolates obtained from Iran by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
... for mass calibration as per manufacturer’s guidelines and showed a typical Escherichia coli DH5 alpha peptide and protein profile with additional protein peaks (data not ...HCCA matrix, and allowed to dry ... See full document
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Discrepancy in MALDI-TOF MS identification of uncommon Gram-negative bacteria from lower respiratory secretions in patients with cystic fibrosis
... nitrogen laser and the spectra were analyzed over a mass range of 2–20 ...strains from both reference and clinical sets were measured and identified by MALDI Biotyper RTC software ... See full document
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