[PDF] Top 20 Virus-Specific Cofactor Requirement and Chimeric Hepatitis C Virus/GB Virus B Nonstructural Protein 3

... A chimeric HCV/GBV-B NS3 gene was constructed by joining two PCR fragments: one consisted of the HCV-1a (H) NS3 protease coding region (amino acids [aa] 1 to 190); the other consisted of the GBV-B ... See full document

... immunodeficiency virus type 1 gp120 V3 domain (60), and changes in HCV amino acid coding are reported to be associated with a number of mutations, clustered at the 59 end of the E2 coding region ...the ... See full document

... FIG 3 TRIB3 negatively regulates HCV ...lysates, protein levels were determined by immunoblot analysis with the indicated ...and protein levels were determined by immuno- blot analysis using the ... See full document

... NS4A cofactor (7) ...lane 3). We investigated whether the HCV NS4A cofactor could further activate the GBV-B NS3 ...(Fig. 3, lanes 3), it was not possible to detect any ... See full document

... related virus to HCV in terms of both its genomic organization and se- quence ...the nonstructural protein 3 (NS3) protease of GBV-B correctly processes the HCV polyprotein (28) and ... See full document

... genome-length chimeric GBV-B/ HCV or HCV-1a/-2a cDNAs or JFH1 control cDNAs containing point substitutions or deletions, we used a primer-based, overlapping PCR mu- tagenesis strategy through which HCV-2a ... See full document

... global protein synthesis, an effect largely attributed to the activation of PKR and phosphorylation of eIF2␣ (3, 4, ...in protein translation levels (data not shown) compared with those of mock- and ... See full document

... capsid protein maturation and lipid droplet targeting involved in particle morphogenesis (23–25) as well as the role of NS3-4A protease in disrupting host innate im- mune responses through the cleavage of the ... See full document

... p7 protein is a small, hydrophobic protein that consists of two transmembrane domains connected by a short stretch of basic residues (10, ...in virus production (12, 34). NS3 is a multifunctional ... See full document

... The chimeric NS4B protein does not significantly alter NS5A association with core and lipid ...core protein on the surfaces of LDs and the recruit- ment of NS5A to these structures appear to be ... See full document

... man hepatitis C (14, 15) and were used as an animal model for validating HCV inhibitors ...viral hepatitis and raised specific immune responses ...major nonstructural proteins NS2, NS3, ... See full document

... core protein release from cells transfected with chimeric genomes, indicated that the association of p7, NS2, and NS5B is required for a crucial step in infectious virus production and ...during ... See full document

... Approximately 3% of the world population is chronically infected with hepatitis C virus ...(HCV). GB virus B (GBV-B), a surrogate model for HCV, causes ... See full document

... is specific and confined to patients with HCV infection, PBMCs from 13 healthy volunteers and from patients with the following liver diseases unrelated to HCV were tested with HCV antigens: acute hepatitis ... See full document

... the requirement for its hyper- phosphorylation diverge between different HCV isolates, mu- tations that are associated with increased replicative fitness of HCV replicons frequently lead to a reduced level of NS5A ... See full document

... NMR-derived constraints and structure calculation. NOE intensities used as input for structure calculations were obtained from the NOESY spectrum re- corded with a 150-ms mixing time and checked for spin diffusion on ... See full document

... epatitis C virus (HCV) is a major etiological agent of chronic liver disease, including cirrhosis and hepatocellular carci- noma ...enveloped virus with a positive-sense, single-stranded RNA that ... See full document

... (Fig. 3 to 5), which can be rescued with an aspartate mutation at S225 or S229 ...S229. Protein kinases, including polo-like kinase 1 (38), calmodulin-dependent kinase II (35), and GSK3 ␤ (22) reported to ... See full document

... Several studies have reported detectable HBV DNA in patients with chronic hepatitis C but negative HBsAg. This so-called "serologically silent" HBV infection or "inappar- ent coinfection" ... See full document

... To elucidate the molecular status of superinfection with these two hepatitis viruses, we cotransfected the full-length or truncated version of HCV structural genes core and envelope 1 to[r] ... See full document