[PDF] Top 20 In vitro study of the NS2-3 protease of hepatitis C virus.
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In vitro study of the NS2-3 protease of hepatitis C virus.
... serine protease crystal structure has indicated that the viral enzyme contains a zinc binding site coordinated by three Cys residues (Cys 1123, 1127, and 1171) and by His 1175 (17, ...serine protease ... See full document
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Inhibitory Effects of Thiazolide Compounds against Hepatitis C virus: in vitro Study
... Abstract Hepatitis C virus (HCV) is estimated to infect nearly 200 million individuals globally, including approximately 10 million in ...in vitro study, we examined the inhibitory ... See full document
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Hepatitis C virus-encoded NS2-3 protease: cleavage-site mutagenesis and requirements for bimolecular cleavage.
... HCV NS2 and NS3 proteins, using multiple antisera, no association of these proteins was detected after cleavage ...of NS2 or NS3 may have precipitated with antiserum to the other protein, but the level of ... See full document
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The Hepatitis C Virus NS2/3 Protease
... the NS2/3 protein and cleavage site plays an important role in the efficiency of NS2/3 ...of NS2/3 activity, likely by altering the positioning of the cleavage site (Darke et ... See full document
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Characterization of the Hepatitis C Virus NS2/3 Processing Reaction by Using a Purified Precursor Protein
... the NS2/3 protease activity resides in a region of the polyprotein that spans from an N-terminal boundary located between residues 898 and 923 to a C-terminal end at residue 1207, even though ... See full document
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The NS2 protein of hepatitis C virus is a transmembrane polypeptide.
... in vitro cleavage at the NS2-3 ...in vitro synthesis of the NS2-3 polyproteins of these two strains clearly shows that, indepen- dently of the transcript length, the BK strain ... See full document
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Determinants of the Hepatitis C Virus Nonstructural Protein 2 Protease Domain Required for Production of Infectious Virus
... Although NS2 expression and stability were not dramatically altered by most C-terminal substitutions, it is possible that deletion of L217 changes the structure of NS2 by liberating the C ... See full document
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Tracking the Evolution of Multiple In Vitro Hepatitis C Virus Replicon Variants under Protease Inhibitor Selection Pressure by 454 Deep Sequencing
... NS3/4A protease inhib- itors (reviewed in reference 17) were observed during the se- lection ...NS3 protease inhibitor (7, 24) with a large S2 group and con- sistent with the results from population ... See full document
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Identification of a lead like inhibitor of the hepatitis C virus non-structural NS2 autoprotease
... both NS2-mediated proteolysis in vitro (Fig. 1B), and NS2-dependent HCV genome replication in a cell based assay ...on NS2-3 ...in vitro transcripts of a JFH-1 derived ... See full document
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Protease Inhibitors Block Multiple Functions of the NS3/4A Protease-Helicase during the Hepatitis C Virus Life Cycle
... and virus production prior to egress at a late stage during assembly or particle ...the protease domain during RNA ...the protease domain of NS3 is required for efficient unwinding of double-stranded ... See full document
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In Vitro Selection and Characterization of Hepatitis C Virus Serine Protease Variants Resistant to an Active-Site Peptide Inhibitor
... The mechanisms by which replacements of aspartate 168 induce resistance to compound 1 are not obvious, and our data provide only hints as to the roles of the different substituents of compound 1 as resistance ... See full document
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Chimeric Sindbis viruses dependent on the NS3 protease of hepatitis C virus.
... a C protein inactivated by alanine substitution of the catalytic serine (21) and containing eight additional amino acid substi- tutions resulting in the transformation of the Sindbis virus C/PE2 ... See full document
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Specific Interaction of Hepatitis C Virus Protease/Helicase NS3 with the 3′-Terminal Sequences of Viral Positive- and Negative-Strand RNA
... Hepatitis C virus (HCV) is the primary causative agent of parenterally transmitted non-A, non-B hepatitis and affects a significant part of the world’s ...chronic hepatitis, cirrhosis ... See full document
14
Generation and Characterization of a Hepatitis C Virus NS3 Protease-Dependent Bovine Viral Diarrhea Virus
... NS3 protease was deleted during serial passages of the chimeric viruses, the infected cells at different passages were subjected to Western blot ...NS3 protease (recognized by the rabbit polyclonal ... See full document
9
Multicenter Quality Control of Hepatitis C Virus Protease Inhibitor Resistance Genotyping
... At the time this national quality control program was started, very few serum samples were available from patients who had been treated with a protease inhibitor and had experienced a viral breakthrough or relapse ... See full document
6
Infection of Common Marmosets with GB Virus B Chimeric Virus Encoding the Major Nonstructural Proteins NS2 to NS4A of Hepatitis C Virus
... the study of hepatitis C virus infection and related liver disease were extensively described (2, 21), including chimpanzees for study of the immune re- sponse and humanized mice for ... See full document
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Hepatitis C Virus NS2 Protein Is Phosphorylated by the Protein Kinase CK2 and Targeted for Degradation to the Proteasome
... in NS2. By computational analysis using the Net- Phos 2.0 Server (3) located at the ExPASy Proteomics tools home page ...of NS2 had the highest proba- bility to be ...of NS2 (a serine at ... See full document
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Hepatitis C Virus NS2 Protein Serves as a Scaffold for Virus Assembly by Interacting with both Structural and Nonstructural Proteins
... of NS2 and its interaction with both structural and nonstructural ...with NS2 but not NS3 ...of NS2 by altering its topology with respect to mem- ...that NS2 is a transmem- brane protein with ... See full document
12
An amino-terminal domain of the hepatitis C virus NS3 protease is essential for interaction with NS4A.
... To study the intra- and intermolecular proteolytic activities associated with the two precursors, either pCite(NS3- 4A) or pCite(NS3[ D N1055]-4A) was cotransfected with pCite ... See full document
9
PCR Based In Vitro Synthesis of Hepatitis C Virus NS3 Protease for Rapid Phenotypic Resistance Testing of Protease Inhibitors
... the hepatitis C virus (HCV) affects an estimated 160 million individuals worldwide (1) and leads to severe liver diseases, such as fibrosis, cirrhosis, and hepatocellular carcinoma ...chronic ... See full document
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