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Plasmin and plasminogen activator inhibitor type 1 promote cellular motility by regulating the interaction between the urokinase receptor and vitronectin

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Figure

Figure 1. Effect of PAI-1 on (circlea) cellular binding of, (b), cellular adhe-sion to, and (c) soluble uPAR binding to vitronectin
Figure 2. PAI-1 promotes detachment of (transfected 293 cells from vitronectin. (with TGF-a) U937 and (b) uPAR-a) U937 cells were primed �1/D3 and pro-uPA in 10% FBS for 24 h; ATF was substi-tuted for pro-uPA in the samples containing 20 nM PAI-1
Figure 3. Migration of uPAR-expressing U937 (10 Polycarbonate filters precoated with 10 10were blocked with PBS/BSA and loaded in a chemotaxis chamber
Figure 4. Effect of plasmin cleavage on U937 cell binding of and ad-tin. Cellular adherence to adsorbed unlabeled vitronectin treated with plasmin as described above was determined in the presence of 10 nM pro-uPA and is expressed in absorbance units

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