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Notch1 signaling in NOTCH1-mutated mantle cell lymphoma depends on Delta-Like ligand 4 and is a potential target for specific antibody therapy

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Figure

Fig. 1 Activation of Notch1 signaling can effectively be achieved by stimulation with DLL4 inmesenchymal stem cells expressing DLL4 (OP9-DLL4) or GFP-transfected control cells for 48 h and protein expression of cleaved Notch1 wasanalyzed by Western Blot
Fig. 2 Treatment ofMCL cells andwith human recombinant DLL4 (4 NOTCH1-mutated MCL cells with OMP-52M51 effectively prevents DLL4-dependent activation of Notch1
Fig. 3 OMP-52M51 significantly impedes DLL4-induced upregulation of numerous genes involved in lymphoid biology, lymphomagenesis anddisease progression mimicking the signature of NOTCH mutated MCL lymph nodes
Fig. 4 (See legend on next page.)
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