2D gel
Comparison of two label-free global quantitation methods, APEX and 2D gel electrophoresis, applied to the Shigella dysenteriae proteome
... and 2D gel datasets decreased with high protein ...in 2D gels ...in 2D gels. In the 2D gel dataset, ...in 2D gels were ...where 2D gels overestimated proteins in ...
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A Proposed Relational Fuzzy C Means Algorithm Applied to 2D Gel Image Segmentation
... (2D gel electrophoresis image of the first sample of Patient- Human leukemias) at C=2, and the results of applying the proposed Relational Fuzzy C- Means (RFCM) segmentation algorithm on at C = 8 and β= 20, ...
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Interactions by 2D Gel Electrophoresis Overlap (iGEO): a novel high fidelity approach to identify constituents of protein complexes
... dimensional gel electrophoresis (2D-GE) We used a two-stage approach to analyze spot patterns of 2D ...single 2D gel to minimize gel-to-gel variation in spot ...standard ...
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A Review on 2D Gel Electrophoresis: A Protein Identification Technique
... a gel at right angles ought to provides a abundant larger degree of resolution than is feasible with either ...by gel electrophoresis however conjointly by activity and capillary ...
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Three Isoforms of Cyclophilin A Associated with Human Immunodeficiency Virus Type 1 Were Found by Proteomics by Using Two-Dimensional Gel Electrophoresis and Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry
... The gel was silver stained. 2D SDS-PAGE standards (Nippon Bio-Rad Laboratories) were used for the reproducibility of 2D ...of 2D electrophoresis patterns of different samples. The 2D ...
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A unique protein profile of peripheral neutrophils from COPD patients does not reflect cytokine-induced protein profiles of neutrophils in vitro
... counts. Gel images were cropped using ImageQuantTL 2003 (GE Healthcare), spot detection was performed with DeCyder ...and gel images were matched using DeCyder ...For 2D-gel analysis p < ...
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Simple discovery of bacterial biocatalysts from environmental samples through functional metaproteomics
... Bacterial biocatalysts play a key role in our transition to a bio-based, post-petroleum economy. However, the discovery of new biocatalysts is currently limited by our ability to analyze genomic information and our ...
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Differential analysis of glioblastoma multiforme proteome by a 2D-DIGE approach
... analytical 2D gel, an average of 700 spots corresponding to proteins with a pI between 3 and 10 and a molecular weight from 10 to 250 kDa was detected and 457 spots were matched across the 5 ...
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Algorithms for Peptide Identification from Mixture Tandem Mass Spectra
... (1) Protein sample preparation by 2D-Gel and digestion by enzymes; (2) Peptide mixture separation by Liquid Chromatography; (3) Survey scan generation in tandem mass spectrometer; (4) Pe[r] ...
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Extrachromosomal circles of satellite repeats and 5S ribosomal DNA in human cells
... eccDNA homologous to an all centromere probe directed to all variants of alpha satellite is readily detected in every human cell line tested. The 2D gel conditions used in this study can resolve circular ...
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Synthesis of Chlorophyll-Binding Proteins in a Fully Segregated Δycf54 Strain of the Cyanobacterium Synechocystis PCC 6803
... a 2D gel (Figure ...our 2D gel shows that levels of Chl synthase, ChlG, HliD, and Ycf39, components of a chlorophyll biosynthetic/membrane insertase assembly complex (Chidgey et ...
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The Replisome Pausing Factor Timeless Is Required for Episomal Maintenance of Latent Epstein-Barr Virus
... Previous studies found evidence that replication forks pause and frequently terminate at OriP. This conclusion is based on the 2D agarose gel (21, 24, 42), as well as single-molecule analysis of replicating ...
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Ocular Proteomics with Emphasis on Two-Dimensional Gel Electrophoresis and Mass Spectrometry
... of 2D-DIGE, ICAT and iTRAQ showed that all three techniques produced fairly accurate quantitative results ...single 2D-gel spot posed problems for spot bound- ary and relative quantitation ...
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Improving image analysis in 2DGE-based redox proteomics by labeling protein carbonyl with fluorescent hydroxylamine
... and 2D Western ...on gel or oxidized protein on 2D Western blot per ...protein 2D Western blot images and total protein 2D gel images, and matching of the FHA labeled ...
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An investigation of plasma collection, stabilization, and storage procedures for proteomic analysis of clinical samples
... of 2D-PAGE and image anal- ysis for clinical samples are many. 2D-PAGE is not a high-throughput method, and image analysis—spot detection and matching— remains less than ...than 2D-PAGE of bacterial ...
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Secretome analysis of rice suspension-cultured cells infected by Xanthomonas oryzae pv.oryza (Xoo)
... The rice - Xoo interaction is a classical model for studying plant - pathogen interactions. Here, we first use 2D-DIGE to analyze differentially expressed secreted proteins in sus- ceptible rice ...
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Generalization technique for 2D+Scale DHE data model
... dataset. It also preserves the topology of the horizontal plane and semantic within the scale axis. However, the tGAP structure (linking appropriate 2D objects at different levels of detail) is not solving the ...
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Distance sampling detection functions : 2D or not 2D?
... the 2D method to be reliable when p(0) is equal to, or close to 1, but it has limited ability to deal with p(0) < ...the 2D line transect estimator can be seen as kind of removal method estimator, and ...
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Original Article Identification of phosphorylated MYL12B as a potential plasma biomarker for septic acute kidney injury using a quantitative proteomic approach
... differential gel electrophoresis (2D-DIGE), and 4 up-regulated proteins, which were actin (ACTB), myosin regulatory light chain 12B (MYL12B), myosin regulatory light polypeptide 9 (MYL9), and myosin ...
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Observation of strain effect on the suspended graphene by polarized Raman spectroscopy
... phenomenon is explained by a proposed mode in which the peak is effectively contributed by an unstrained and two uniaxial-strained sub-areas. The two axes are tensile strain. Compared to the unstrained sub-mode frequency ...
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