40 large T-Antigen
Self-assembly of simian virus 40 large T antigen oligomers by divalent cations.
... 531-538 0022-538X/83/020531-08$02.00/0 Copyright © 1983, American Society for Microbiology Self-Assembly of Simian Virus 40 Large T Antigen Oligomers by Divalent Cations MATHIAS MONTENAR[r] ...
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Simian virus 40 large T antigen oligomers: analysis of electrophoresis in the absence of detergent.
... 711-716 0022-538X/85/120711-06$02.00/0 Copyright © 1985, American Society for Microbiology Simian Virus 40 Large T Antigen Oligomers: Analysis of Electrophoresis in the Absence of Deterg[r] ...
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ATP enhances the binding of simian virus 40 large T antigen to the origin of replication.
... 12 0022-538X/87/123649-06$02.00/0 Copyright © 1987, American Society for Microbiology ATP Enhances the Binding of Simian Virus 40 Large T Antigen the Origin of Replication to SWATI PALIT[r] ...
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Simian virus 40 large T antigen associates with cyclin A and p33cdk2.
... The most likely candidates for such a linker are the recessive oncoproteins pRb and p107, both of which can bind cyclin A and p33cdk2 independently of viral oncoproteins 14, 23, but it i[r] ...
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A novel translational regulation function for the simian virus 40 large-T antigen gene.
... SV40 large-T antigen might be me- diated by the mRNA that encodes the large-T antigen, rather than by the ...mutant large-T plasmid (pR137) was constructed in which ...
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Crystal Structure of the Simian Virus 40 Large T-Antigen Origin-Binding Domain
... SV40 large T-antigen origin binding ...of T-ag obd with corresponding secondary-structure assignments (using the program DSSP) indicated by cylinders (alpha helices) or arrows (beta sheets); ...
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Identification of a novel antiapoptotic functional domain in simian virus 40 large T antigen.
... reference 40 for a review); however, it is becoming increasingly clear that p53-indepen- dent mechanisms of adenovirus-induced apoptosis also exist (33, ...
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Functional characterization of temperature-sensitive mutants of simian virus 40 large T antigen.
... Purified T antigens T ag were either not exposed - or exposed + to a heat shock for 10 min at 39°C, incubated in replication buffer containing plasmids with the SV40 origin of replicatio[r] ...
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Identification of a region of simian virus 40 large T antigen required for cell transformation.
... However, mutants which lack origin-binding activity retain the ability to transform both primary and established cells phenotypically, indicating that It has been known for some time tha[r] ...
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Oligomerization and origin DNA-binding activity of simian virus 40 large T antigen.
... In contrast, studies carried out with lytic T-antigen subclasses separated by zone velocity sedimentation or phosphocellulose chromatography showed that newly synthesized 5 to 7S T antig[r] ...
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Monoclonal antibodies specific for the carboxy terminus of simian virus 40 large T antigen.
... Peptides used for immunization of mice and in characterization of antibodies Designation Amino acid sequences corresponding to Tthe of SV40 large carboxy-terminal region Lys-Thrz Hexamer[r] ...
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An oligomeric form of simian virus 40 large T-antigen is immunologically related to the cellular tumor antigen p53.
... Preliminary data suggest that PAb11O4 can bind to D2 protein, which lacks the N-terminal 82 amino acids of large T, whereas, in blocking studies with anti-T antibodies of the PAb200 seri[r] ...
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Linker insertion mutants of simian virus 40 large T antigen that show trans-dominant interference with wild-type large T antigen map to multiple sites within the T-antigen gene.
... It also plays central roles in immortalization of primary cells 61, 62 and in the malignant Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Linker insertion mutants aff[r] ...
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The zinc finger region of simian virus 40 large T antigen.
... Such a change in conformation could affect multiple independent functions, including origin binding, transformation, and the function of the active site element of the zinc finger in rep[r] ...
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Mapping the transcriptional transactivation function of simian virus 40 large T antigen.
... Two mutants with lesions in the DNA-binding domain that were unable to bind to SV40 DNA were completely defective for transactivation of both promoters, while a third mutant with a lesio[r] ...
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Measurements of the molecular size of the simian virus 40 large T antigen.
... This would be 218 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest A measure of the molecular weight of the large simian virus 40 T antigen was sought by SDS-polyacrylam[r] ...
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Immunological Cross-Reactivity Between Simian Virus 40 Large T Antigen and D2 Hybrid T Antigen
... Proteins were immunoprecipitated from these extracts by using the antiserum produced in rabbits against purified D2 hybrid T antigen rabbit anti-D2, and these proteins were compared with[r] ...
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Use of simian virus 40 large T-beta-galactosidase fusion proteins in an immunochemical analysis of simian virus 40 large T antigen.
... Inhibition of binding of '25I-labeled pure large T antigen to the immobilized antibody in the presence of the fusion protein cell extract containing ,-galactosidase alone was used as a c[r] ...
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Quantitative Analysis of the Binding of Simian Virus 40 Large T Antigen to DNA
... that T-ag could interact, albeit more weakly, with two noncanonical se- quences, both 5 ⬘ -TCGGC-3 ⬘ , present in the origin ...by 40%, whereas an identical substitution in P4 (to 5 ⬘ -GAAGC- 3 ⬘ ) ...
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Simian virus 40 large T antigen: the puzzle, the pieces, and the emerging picture.
... Overexpres1289 Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest INTRODUCTION: THE PUZZLE In cultured monkey cells infected by simian virus 40 SV40, the viral regulatory p[r] ...
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