Activity-based protein profiling
Activity-based protein profiling and labeling of bacterial monooxygenase enzymes.
... The role bacterial monooxygenase enzymes play in the degradation of polluting substances has long been recognized. While much is known about how these enzymes contribute to contaminant biodegradation, there are currently ...
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Activity-based protein profiling reveals off-target proteins of the FAAH inhibitor BIA 10-2474
... used activity- based protein profiling (ABPP), a chemical proteomic method that employs active site- directed chemical probes ...cellular activity of BIA 10-2474 is unclear, but could ...
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The use of activity based protein profiling to study proteasome biology
... termed activity based protein profiling (ABPP) ...called activity-based probes (ABPs), which create a covalent and irreversible bond with the catalytic active site of the enzyme ...
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Activity-Based Protein Profiling of Alkane-Oxidizing Monooxygenases in Rhodococcus rhodochrous ATCC 21198.
... An activity-based protein profiling (ABPP) method was developed to identify and characterize the gaseous alkane-oxidizing monooxygenases in this ...
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In vivo inactivation of glycosidases by conduritol B epoxide and cyclophellitol as revealed by activity-based protein profiling
... substrates, activity-based probes (ABPs) could be superior tools for this ...competitive activity-based protein profiling (ABPP) approach to identify in vivo target engagement of ...
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Activity-based protein profiling guided identification of urine proteinase 3 activity in subclinical rejection after renal transplantation
... a protein does not necessarily imply functional ...methods based on mass spectrometry or immunoassays generally do not provide information on the functional status of ...of activity-based ...
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Activity-based protein profiling
... Fluorescence polarisation (FluoPol)-ABPP has been developed to perform high-throughput screens and to assess inhibitor kinet- ics (Bachovchin et al., 2009; Lahav et al., 2017). FluoPol mea- sures the apparent size of a ...
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From medicinal chemistry optimisation of antimalarial 2-aryl quinolones to synthesis and application of endoperoxide activity-based protein profiling probes
... 25. O'Neill, P. M.; Mukhtar, A.; Stocks, P. A.; Randle, L. E.; Hindley, S.; Ward, S. A.; Storr, R. C.; Bickley, J. F.; O'Neil, I. A.; Maggs, J. L.; Hughes, R. H.; Winstanley, P. A.; Bray, P. G.; Park, B. K., Isoquine and ...
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Multiplex Fluorescent, Activity-Based Protein Profiling Identifies Active α-Glycosidases and Other Hydrolases in Plants
... dedicated protein database by translating the publicly available RNA sequencing data from Jain et ...the protein N terminus (42 D) as dynamic modifications and the static modi- fication of Cys (57 D, ...
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Advancing understanding of microbial bioenergy conversion processes by activity-based protein profiling
... known protein activities in both sequenced and un- sequenced genomes, and determination of the subcellu- lar localization of probe reactive enzymes [5, ...probe profiling of exo-glycosidases in Pseudomonas ...
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Development of a Multiplexed Activity-Based Protein Profiling Assay to Evaluate Activity of Endocannabinoid Hydrolase Inhibitors
... the activity and selectivity of compounds inhibiting biosynthetic or metabolic enzymes of the ...ed based on previous ...inhibitory activity of LEI104 on DAGL- α and FAAH in this new multiplexed ABPP ...
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Activity-based protein profiling of the hepatitis C virus replication in Huh-7 hepatoma cells using a non-directed active site probe
... Subconfluent cells (80-90%) were washed and pooled with ice-cold 10 mM sodium phosphate buffer, pH 7. The cells were then subjected to Dounce-homogeniza- tion at 30% power (T8 Ika-Werke homogenizer, GMBH, Staufen, ...
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Direct and two-step activity-based profiling of proteases and glycosidases
... ´activity-based protein profiling´ (ABPP), een vakgebied binnen de chemische biologie met als doel de activiteit van enzymen zichtbaar te maken en te kwantificeren in de context van een ...
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Evaluation of clinical colon carcinoma using activity-based proteomic profiling
... an activity-based pro- teomic profiling (ABPP) approach for drug target discovery in clinical colorectal car- ...tagged, activity-based chemical probe to comprehen- sively scan the ...
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<p>iTRAQ-based quantitative protein expression profiling of biomarkers in childhood B-cell and T-cell acute lymphoblastic leukemia</p>
... leukemia activity in ALL patients as its concentration likely re fl ects the total mass of leukemic cells and its synthesis may be upregulated by blast ...disease activity and poor ...leukemia ...
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Kinase Activity Profiling of Gram-Negative Pneumonia
... Kinase activity arrays enable the analysis of 1,024 con- sensus sequences of protein kinase ...kinase activity array on whole lung lysates, cellular kinase activities were determined in a mouse model ...
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Massive metagenomic data analysis using abundance-based machine learning
... one-node based calculation in Table 2. The wall- clock time using read-based approach can be reduced and near linearly scaled if multi-node cluster is ...
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Cytotoxic activity of crude extracts from Datura stramonium’s fungal endophytes against A549 lung carcinoma and UMG87 glioblastoma cell lines and LC QTOF MS/MS based metabolite profiling
... In the xCELLigence assay, the cytotoxic activity of the crude extract of Alternaria sp. KTDL7 at 100 μg/mL on UMG87 glioblastoma cells was observed to be much higher and not comparable to that of the same extract ...
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Profiling physical activity motivation based on self-determination theory: a cluster analysis approach
... Recently, PA researchers have begun to study moti- vational profiles based on SDT using cluster analysis. Cluster analysis is a statistical method that groups indivi- duals into clusters based on similar ...
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Original Article Protein profiling detection in serum of silica-exposed population by liquid-chip time of flight mass spectrometry
... [4] Zhang X, Leung SM, Morris CR and Shigenaga MK. Evaluation of a novel, integrated approach using functionalized magnetic beads, bench- top MALDI-TOF-MS with prestructured sample supports, and pattern recognition ...
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