Adaptor Protein Complex 3
Characterization of the adaptor-related protein complex, AP-3.
... the complex consists not only of b -NAP and p47, but also of two other proteins of z 160 (p160) and z 25 kD (p25), making it a heterotetramer like an adaptor ...this complex is shown in ...this ...
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Interaction of the Bovine Papillomavirus E6 Protein with the Clathrin Adaptor Complex AP-1
... AP-1 complex was present in the crude cyto- solic and membrane-bound fractions, as judged by immuno- precipitation of AP-1 ...100,000 3 g for 1 h to obtain free cytosolic ...
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The Mouse Homolog of the Bovine Leukemia Virus Receptor Is Closely Related to the δ Subunit of Adaptor-Related Protein Complex AP-3, Not Associated with the Cell Surface
... AP-3 adaptor-related protein complex (32) than to the bovine BLVR ...BLVR protein pre- dicted a type I TM protein with signal peptides and a hydro- phobic TM region (3, ...
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Complex interactions of the domains of the p85 adaptor subunit of phosphoinositide 3-kinase
... Bound protein was eluted at a flow rate of 2ml/min, and ...bound protein was eluted with 30ml 20mM Tris pH8, 5mM DTT followed by a 30ml linear gradient from 0 to ...concentrated protein was stored at ...
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Interstitial Lung Disease and Pulmonary Fibrosis in Hermansky-Pudlak Syndrome Type 2, an Adaptor Protein-3 Complex Disease
... and his father is half African American, one-fourth Chitimacha Indian, and one- fourth Houma Indian. There is no known history of consanguinity. Genetic analy- sis demonstrated compound heterozy- gous nonsense mutations ...
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Prevalence of Plasmodium falciparum delayed clearance associated polymorphisms in adaptor protein complex 2 mu subunit (pfap2mu) and ubiquitin specific protease 1 (pfubp1) genes in Ghanaian isolates
... Malaria is still a debilitating disease, especially in sub- Saharan Africa (sSA) where there were 212 million cases and 429,000 malaria-related deaths in 2015 [1]. There has been a 21 and 29% reduction in morbidity and ...
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Varicella-Zoster Virus ORF9p Binding to Cellular Adaptor Protein Complex 1 Is Important for Viral Infectivity
... FIG 5 The L231A mutation strongly impacts infectivity in MeWo and MRC-5 cells. VZV-ORF9-V5 (WT) and mutant strain infectivity was assessed in MeWo cells (A and B) or MRC-5 cells (C to E). (A) Infection focus measurement ...
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Localization of the AP-3 adaptor complex defines a novel endosomal exit site for lysosomal membrane proteins
... Mouse mAbs against the AP-3 subunit were generated by immunizing fe- male BALB/c mice with a GST fusion protein corresponding to the hinge domain of the subunit (aa 608–800 of human -adaptin). Fusion of the ...
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Recruitment of the Adaptor Protein 2 Complex by the Human Immunodeficiency Virus Type 2 Envelope Protein Is Necessary for High Levels of Virus Release
... Generation of virus particles and Western blot analysis. HeLa cells grown to 80 to 90% confluence were transiently transfected with the appropriate plasmids using Lipofectamine 2000 (Invitrogen, Carlsbad, Calif.) as ...
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Eps15: a multifunctional adaptor protein regulating intracellular trafficking
... Since Eps15 is constitutively associated with the coated pit, the induced binding with the activated receptors would result in the recruitment of these receptors into the coated pit. This is in line with the observation ...
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Schmid, Maria (2008): Coordination of endoplasmic reticulum and mRNA localization in Saccharomyces cerevisiae. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... motor protein Myo4 and the adaptor protein She3p were recently identified to be essential factors for segregation of cortial ER into daughter cells (Estrada et ...the adaptor protein ...
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Cell cycle and cell growth regulation by the CUL4-DDB1-ROC1 ubiquitin ligases
... finger protein: ROC1 (RING of Cullins, also known as Hrt1 and Rbx1), ROC2 or APC11 (Deshaies, 1999; Jackson et ...targeting protein)— either directly or through an adaptor protein or ...
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Cleavage of the Adaptor Protein TRIF by Enterovirus 71 3C Inhibits Antiviral Responses Mediated by Toll-Like Receptor 3
... 3C protein, which disrupts the formation of a functional RIG-I ...receptor 3 (TLR3) via a distinct ...factor 3 and drastically suppresses interferon-stimulated gene ...domain-containing ...
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Involvement of the adaptor protein 3 complex in lignocellulase secretion in Neurospora crassa revealed by comparative genomic screening
... a protein kinase), whose deletion increased the amount of secreted protein by approximately 35% compared with WT ...a protein predicted as a G2/mitotic-specific cyclin), whose deletion increased ...
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Regulation of dematin by the p38 MAPK and the adaptor protein 14 3 3
... by protein kinase D ...severing protein, through dephosphorylation of Ser 3 (Andrianantoandro & Pollard, ...binding protein found in a number of actin structures that interacts with the ...
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Defective Pigment Granule Biogenesis and Aberrant Behavior Caused by Mutations in the Drosophila AP-3β Adaptin Gene ruby
... Lysosomal protein trafficking is a fundamental process conserved from yeast to ...heterotetrameric adaptor protein complex AP-3 involved in vesicle ...
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miR 19a: an effective regulator of SOCS3 and enhancer of JAK STAT signalling
... conserved 3’UTR miR-19a target sequences in several JAK-STAT associated genes, including SOCS1, SOCS3, SOCS5 and Cullin (Cul) ...and protein, while a miR-19a antagomir specifically reversed its inhibitory ...
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Autoimmune gait disturbance accompanying adaptor protein-3B2-IgG
... None of patient sera 1–10, or 4 available CSF, demonstrated IgG binding to live hippocampal neurons (figure 2). In con- trast, CSF from the NMDAR-positive patient bound strongly to live neurons. Sera from patients 1–10 ...
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Adaptor protein LNK promotes anaplastic thyroid carcinoma cell growth via 14-3-3 ε/γ binding
... The protein concentra- tion in the lysate was measured by a BCA protein ...μg protein was separated on a 9% SDS–poly- acrylamide gel by electrophoresis, and transferred to a polyvinylidene fluoride ...
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ENTH and ANTH domain proteins participate in AP2 independent clathrin mediated endocytosis
... Clathrin-mediated endocytosis (CME) is a major route of entry into eukaryotic cells. A core of evolutionarily ancient genes encodes many components of this system but much of our mechanistic understanding of CME is ...
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