Adeno-associated virus (AAV)
Selective Cleavage of AAVS1 Substrates by the Adeno-Associated Virus Type 2 Rep68 Protein Is Dependent on Topological and Sequence Constraints
... The adeno-associated virus type 2 (AAV-2) Rep78 and Rep68 proteins are required for replication of the virus as well as its site-specific integration into a unique site, called AAVS1, ...
12
Packaging of Human Chromosome 19-Specific Adeno-Associated Virus (AAV) Integration Sites in AAV Virions during AAV Wild-Type and Recombinant AAV Vector Production
... Adeno-associated virus (AAV) has evolved a biphasic life cycle to ensure persistence in its primate ...helper virus, adenovirus (Ad), or herpesvirus for productive infection ...helper, ...
7
Structure of simian virus 40-adeno-associated virus recombinant genomes.
... 56, 1985 STRUCTURE OF SV 40-ADENO-ASSOCIATED VIRUS GENOMES DISCUSSION The SV40-AAV recombinants classified as group A type, which arose from cells either cotransfected with SV40 and AAV [r] ...
9
Marker Rescue of Adeno-Associated Virus (AAV) Capsid Mutants: a Novel Approach for Chimeric AAV Production
... novel adeno-associated virus (AAV) vectors. AAV serotype 2 (AAV2) clones containing wild-type terminal repeats, an intact rep gene, and a mutated cap gene, served as the template for ...
10
Adeno-Associated Virus Type 2-Mediated Transduction of Human Monocyte-Derived Dendritic Cells: Implications for Ex Vivo Immunotherapy
... evaluated adeno-associated virus (AAV) type 2 as a vector for ex vivo gene transfer to human peripheral blood monocyte (MO)-derived ...DCs. AAV is a nonpathogenic parvovirus that ...
9
Interaction of wild-type and mutant adeno-associated virus (AAV) Rep proteins on AAV hairpin DNA.
... of adeno-associated virus type 2 (AAV) bind to AAV terminal repeat hairpin DNA and can mediate site-specific nicking in vitro at the terminal resolution site (trs) within the terminal ...
9
Residues on Adeno-associated Virus Capsid Lumen Dictate Interactions and Compatibility with the Assembly-Activating Protein
... nonautonomous adeno-associated virus (AAV) is not associated with disease, and thus there has historically been less incentive to study basic AAV biology in as great depth as ...
16
An Adeno-Associated Virus (AAV) Initiator Protein, Rep78, Catalyzes the Cleavage and Ligation of Single-Stranded AAV ori DNA
... Adeno-associated virus type 2 (AAV-2), a member of the Parvoviridae family, is a nonenveloped, icosahedral virus with a linear, single-stranded DNA (ssDNA) genome of 4,680 nucle- otides ...
8
Identification of linear DNA sequences that specifically bind the adeno-associated virus Rep protein.
... We find that Rep68 is capable of binding to a linear DNA sequence that is contained within a 25-bp sequence of the A stem of the adeno-associated virus AAV terminal repeat proximal to th[r] ...
10
An Alternate Route for Adeno-associated Virus (AAV) Entry Independent of AAV Receptor
... steer adeno- associated virus (AAV) in its utility as a gene therapy ...diverse AAV serotypes engage their proteinaceous receptor AAVR (KIAA0319L) to establish transduction has been ...
15
Adeno-associated Virus (AAV) Serotypes Have Distinctive Interactions with Domains of the Cellular AAV Receptor
... ABSTRACT Adeno-associated virus (AAV) entry is determined by its interactions with specific surface glycans and a proteinaceous ...receptor(s). Adeno-associated virus ...
17
The 37/67-Kilodalton Laminin Receptor Is a Receptor for Adeno-Associated Virus Serotypes 8, 2, 3, and 9
... Adeno-associated virus serotype 8 (AAV8) is currently emerging as a powerful gene transfer vector, owing to its capability to efficiently transduce many different tissues in ...other AAV ...
6
Charged-to-Alanine Scanning Mutagenesis of the N-Terminal Half of Adeno-Associated Virus Type 2 Rep78 Protein
... Adeno-associated virus (AAV) type 2 (referred to here as AAV) is a nonpathogenic parvovirus with a linear, single- stranded DNA genome of ...in AAV DNA ...
12
Adeno-Associated Virus (AAV) Rep Protein Enhances the Generation of a Recombinant Mini-Adenovirus (Ad) Utilizing an Ad/AAV Hybrid Virus
... quantifying virus particles by measuring the optical density at 260 ...hybrid virus was able to transduce GFP expression to nearly all of the cells in the culture at the same ...physical virus ...
9
Infectious Clones and Vectors Derived from Adeno-Associated Virus (AAV) Serotypes Other Than AAV Type 2
... other AAV serotypes could prove espe- cially useful for transducing cells that are resistant to AAV2 ...each AAV serotype uses a different recep- tor ...other AAV serotypes may have a host range ...
11
Identification of a Functionally Relevant Adeno-Associated Virus Rep68 Oligomeric Interface
... of a dimeric Rep-Rep interaction built from the pseudodimer ob- served in the crystal structure of Rep40 using an extended Rep40 molecule (33). In this model, a large interface that resembled the interface formed by the ...
13
Adeno-associated Virus (AAV) Assembly-Activating Protein Is Not an Essential Requirement for Capsid Assembly of AAV Serotypes 4, 5, and 11
... Western blotting. We seeded HEK 293 cells onto 6-well plates and transfected them with a total of 2 g of a plasmid or a mixture of plasmids using PEI. The plasmids used in each experiment are described in each relevant ...
21
A Comprehensive RNA Sequencing Analysis of the Adeno-Associated Virus (AAV) Type 2 Transcriptome Reveals Novel AAV Transcripts, Splice Variants, and Derived Proteins
... polyadenylated AAV reads in the data set of AAV2/Ad2-coinfected ...the AAV genome with the respective nucleotide positions of the ...strand. AAV( ⫹ )190-320-luc represents the AAV2 p5 promoter in the ...
12
Reducing the Risk of Adeno-Associated Virus (AAV) Vector Mobilization with AAV Type 5 Vectors
... from AAV serotypes that correspond to the plasmid number ...simian virus 40 polyadenylation [poly(A)] signal, flanked by AAV2 TR (TR2-eGFP) or AAV5 TR (TR5-eGFP) (see ...
11
Original Article Prevalence of neutralizing antibodies against AAV8, AAV9, and AAV843 in a Chinese population
... Abstract: Adeno-associated virus (AAV) is a tiny, non-enveloped, and single-stranded DNA virus that requires helper viruses to promote efficient ...Recombinant AAV has been ...
10