Adeno-Associated Virus Type 2
Mutational Analysis of Adeno-Associated Virus Type 2 Rep68 Protein Endonuclease Activity on Partially Single-Stranded Substrates
... of adeno-associated virus type 2 (AAV) cuts at the terminal resolution site (trs) within the hairpin structure formed by the AAV inverted terminal ...
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The Adeno-Associated Virus Type 2 Regulatory Proteins Rep78 and Rep68 Interact with the Transcriptional Coactivator PC4
... The adeno-associated virus type 2 (AAV-2) Rep78/Rep68 regulatory proteins are pleiotropic effectors of viral and cellular DNA replication, of cellular transformation by viral and ...
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Adeno-Associated Virus Type 2 Rep Protein Inhibits Human Papillomavirus Type 16 E2 Recruitment of the Transcriptional Coactivator p300
... human adeno-associated virus type 2 (AAV2) is a possible protective factor in the development of cervical carcinomas associated with human papillomaviruses ...HPV type 16 ...
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High Throughput Screening for the Enhancement of Adeno-Associated Virus Type 2 Transduction
... Adeno-associated virus (AAV) is a promising vector for human gene ...of adeno-associated virus type 2 (AAV2) ...the virus-receptor interaction, the viral DNA ...
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Adeno-Associated Virus Type 2 Protein Interactions: Formation of Pre-Encapsidation Complexes
... nonstructural adeno-associated virus type 2 Rep proteins are known to control viral replication and thus provide the single-stranded DNA genomes required for packaging into preformed ...
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Adeno-associated virus type 2-mediated transfer of ecotropic retrovirus receptor cDNA allows ecotropic retroviral transduction of established and primary human cells.
... recombinant adeno-associated virus type 2 (AAV) vector containing ecotropic retroviral receptor (ecoR) cDNA under the control of the Rous sarcoma virus (RSV) long terminal repeat ...
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Adeno-Associated Virus Type 2-Mediated Gene Transfer: Correlation of Tyrosine Phosphorylation of the Cellular Single-Stranded D Sequence-Binding Protein with Transgene Expression in Human Cells In Vitro and Murine Tissues In Vivo
... the adeno-associated virus type 2 (AAV)-based vector system has gained attention as a potentially useful alternative to the more commonly used retroviral and adenoviral vectors for ...
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Adeno-associated virus type 2 DNA replication in vivo: mutation analyses of the D sequence in viral inverted terminal repeats.
... The adeno-associated virus type 2 (AAV) genome contains inverted terminal repeats (ITRs) of 145 nucleo- tides. The terminal 125 nucleotides of each ITR form palindromic hairpin (HP) ...
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Genetics of adeno-associated virus: isolation and preliminary characterization of adeno-associated virus type 2 mutants.
... Among these mutants, we distinguished at least three phenotypes: i mutants that were defective in their ability to rescue or replicate the AAV genome from the recombinant plasmids rep mu[r] ...
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Mutational analysis of the adeno-associated virus type 2 Rep68 protein helicase motifs.
... parent conflict with a previous report in which NTPs other than ATP could be utilized by Rep68 for its trs endonuclease activity. Im and Muzyczka (20) demonstrated that CTP, GTP, and UTP were also cofactors for ...
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A novel terminal resolution-like site in the adeno-associated virus type 2 genome.
... helper virus; otherwise, AAV cannot establish a latent ...helper virus pro- teins are not directly involved in AAV DNA rescue and rep- ...helper virus, are involved in the rescue and replication of ...
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Hepatocyte Growth Factor Receptor Is a Coreceptor for Adeno-Associated Virus Type 2 Infection
... are associated with the Golgi apparatus (16, ...(lanes 2 to 7) are known to lack the  subunit of ...1, 2, and 5); immunoprecipitated  chain protein of c-Met (lanes 3, 4, 6, and 7); control ...
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Identification of Rep-Associated Factors in Herpes Simplex Virus Type 1-Induced Adeno-Associated Virus Type 2 Replication Compartments
... Factors involved in DNA repair. Viruses that replicate in the nucleus, such as adenovirus and HSV-1, have been shown to significantly interfere with the DNA repair machinery and the DNA damage response (DDR) to inhibit ...
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Differential Contribution of Adeno-Associated Virus Type 2 Rep Protein Expression and Nucleic Acid Elements to Inhibition of Adenoviral Replication in cis and in trans
... the replication of the respective helper, which is especially evident in the case of adenovirus (18, 19). The AAV-mediated inhibition of adenoviral replication has been attributed to the expression of the Rep proteins ...
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Induction of differentiation-associated changes in established human cells by infection with adeno-associated virus type 2.
... The mechanism of AAV-2-induced differentiation is not clear, and we do not know whether cessation of cell proliferation marked by a decrease of c-myc expression, arrest of cells in the G[r] ...
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Mutational Analysis of Narrow Pores at the Fivefold Symmetry Axes of Adeno-Associated Virus Type 2 Capsids Reveals a Dual Role in Genome Packaging and Activation of Phospholipase A2 Activity
... In this study, we showed that mutation of amino acids sur- rounding the fivefold pore of AAV2 capsids impaired genome packaging (Fig. 3 and Table 1). These data indicated that the pores may form the portal for DNA ...
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Adeno associated virus type 2 preferentially integrates single genome copies with defined breakpoints
... The structurally complex, repetitive, and GC-rich nature of these sequences may hinder capture of the entire junction-population. We have taken many steps to mitigate these effects. These steps included using: short ...
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Characterization of the Capsid Protein Glycosylation of Adeno-Associated Virus Type 2 by High-Resolution Mass Spectrometry
... cell type and with the higher in vitro propagation levels that could saturate posttranslational modification ...the virus was har- vested 48 h postinfection ...the virus produced would have glycosyl- ...
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Mutants at the 2-Fold Interface of Adeno-associated Virus Type 2 (AAV2) Structural Proteins Suggest a Role in Viral Transcription for AAV Capsids
... were 2 and 2 logs defective, ...produced virus titers that were within a factor of 2 of wild-type ...in virus bound to the A20 ...
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Dynamin Is Required for Recombinant Adeno-Associated Virus Type 2 Infection
... Recombinant adeno-associated virus (rAAV) vectors for gene therapy of inherited disorders have demon- strated considerable potential for molecular ...AAV type 2 (AAV-2) has begun ...
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