Adenovirus DNA replication
A study of the in vitro initiation of adenovirus DNA replication
... studying adenovirus DNA replication arose from the concept that analysis of the mechanism and control of replication of the relatively small and simple genomes of the DNA viruses which ...
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Topoisomerase I and II cleavage of adenovirus DNA in vivo: both topoisomerase activities appear to be required for adenovirus DNA replication.
... The efficiency of topoisomerase II cleavage increased as the rate of adenovirus DNA replication increased and then decreased with the decreasing rate of replication late in the infection[r] ...
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Initiation of adenovirus DNA replication.
... Adenovirus DNA was synthesized in vitro in the presence of [3H]dTTP (-4*) either with protein-free viral DNA (A) or with the DNA-protein complex as the template (B, C, and D). Samples we[r] ...
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Mechanisms of adenovirus DNA replication
... of adenovirus have been found to infect humans causing acute respiratory, gastrointestinal, ocular and urinary ...of adenovirus infections in man are seldom associated with fatal diseases, (the exception ...
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Replication-Uncoupled Histone Deposition during Adenovirus DNA Replication
... the adenovirus genome DNA plays critical roles in its genome ...viral DNA is associated with both viral core protein VII and cellular his- ...viral DNA is also associated with ...the ...
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Conserved Sequences at the Origin of Adenovirus DNA Replication
... Sequence of inverted terminal repetitions from different adenovi- ruses: demonstration of conserved sequences and homol- ogy between SA 7 termini and SV40 DNA. Presence of protein at the[r] ...
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Functional characterization of thermolabile DNA-binding proteins that affect adenovirus DNA replication.
... Biochemical studies of purified Ad2+NDlts23 DBP showed that this protein was defective for elongation but not initiation of replication in a cell-free replication system consisting of pu[r] ...
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Adenovirus DNA replication in vitro: a protein linked to the 5' end of nascent DNA strands.
... Double-stranded, terminal restriction fragments containing one parental and one labeled nascent strand bound to BND-cellulose because of a protein associated with them. After replication[r] ...
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Inhibition of adenovirus DNA replication by vesicular stomatitis virus leader RNA.
... Specific replication in the presence of leader RNA was restored when the concentration of adenovirus-infected or uninfected nuclear extract was increased or by the addition of purified p[r] ...
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An Adenovirus DNA Replication Factor, but Not Incoming Genome Complexes, Targets PML Nuclear Bodies
... replicating DNA viruses use the domains as deposition sites for the incoming viral genomes and/or as sites for viral DNA replication, suggesting that PML-NBs are functionally relevant during early ...
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Differential Requirements of the C Terminus of Nbs1 in Suppressing Adenovirus DNA Replication and Promoting Concatemer Formation
... for DNA replication and late protein ...a DNA damage response, accumulation of cellular repair factors in foci at viral replication centers, and joining together of viral genomes into ...
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Adenovirus preterminal protein synthesized in COS cells from cloned DNA is active in DNA replication in vitro.
... Expression of enzymatically active adenovirus DNA polymerase from cloned DNA requires sequences upstream of the main open reading frame. Adenovirus DNA replication in vitro: a[r] ...
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Widespread Phosphorylation of Histone H2AX by Species C Adenovirus Infection Requires Viral DNA Replication
... viral DNA replication, it seems likely that some feature of the viral DNA must be recognized promptly after delivery into the cell and trigger some facet of the DNA damage ...viral ...
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Adenovirus type 2 DNA replication. II. Termini of DNA replication.
... labeled viral DNA, extracted from infected cells, into mature and replicating forms. Exper-[r] ...
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Bidirectional replication of adenovirus type 2 DNA.
... The models of adenovirus replication as a linear molecule fail to provide a mechanism for the synthesis of the 5' ends of the DNA. If RNA is a primer in this system, there would not be a[r] ...
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Termination sites for adenovirus type 2 DNA replication.
... The fragment order (11) is shown on top of the figure. Fragments HpaI-A and HpaI-B were not separated.. Relative amounts of [3HJthymidi different fragments obtained by cleavage completed[r] ...
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Location of the Origin of DNA Replication in Adenovirus Type 2
... If one harvests the heavy side of the Ad2+ND, GC-rich half molecules after cesium chloride equilibrium centrifuga- tion and the light half of the Ad2+ND, AT-rich molecules, it can be sho[r] ...
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Adenovirus type 2 DNA replication. I. Evidence for discontinuous DNA synthesis.
... Analysis by alkaline sucrose gradient cen- trifugation of viral DNA labeled in Ad2-infected HeLa cells in the presence of HU. (Upper panel) Sedimenta-[r] ...
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Persistence of recombinant adenovirus in vivo is not dependent on vector DNA replication.
... vector DNA in noncomplementing cell lines and cultured primary hepatocytes (11, 25) and the loss of vector DNA upon inhibition of virus replication, led to the hypothesis that vector DNA ...
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In vitro termination of adenovirus DNA synthesis by a soluble replication complex.
... DNA in the replication complex was labeled in vitro for 20 min with [3H]dTTP, then purified and digested with restriction enzyme (after addition of uniformly labeled Ad2 /'4C]DNA), and d[r] ...
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