Adenovirus type 4 DNA replication
Identification of two distinct regions within the adenovirus minimal origin of replication that are required for adenovirus type 4 DNA replication in vitro.
... Plasmid templates (100 ng) p4A2, pHR18, and pM9 to pM18 were cleaved with EcoRI and AvaII and incubated with 3 ,ug of cytoplasmic extract from Ad4-infected HeLa cells in a standard elong[r] ...
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Adenovirus Type 5 DNA Binding Protein Stimulates Binding of DNA Polymerase to the Replication Origin
... Ad5 origin as a dsDNA probe. Upon addition of DBP, Pol binding to dsDNA was clearly enhanced, strongly suggesting that DBP can stimulate Pol binding to the origin (Fig. 4). We wondered whether the enhanced ...
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Selective Modification of Adenovirus Replication Can Be Achieved through Rational Mutagenesis of the Adenovirus Type 5 DNA Polymerase
... The I664V Ad5 mutant preferentially lyses cancer cells. We next evaluated the ability of the I664V Ad5 mutant to induce cell killing in a small panel of normal (primary) cells and cancer cells. BJ and HCA-2 primary ...
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A study of the in vitro initiation of adenovirus DNA replication
... human adenovirus serotypes probably reflects a crucial functional constraint on this domain, which makes it a very likely candidate for containing the sequences which are critical for recognition by ...a ...
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Adenovirus type 2 DNA replication. II. Termini of DNA replication.
... labeled viral DNA, extracted from infected cells, into mature and replicating forms. Exper-[r] ...
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Bidirectional replication of adenovirus type 2 DNA.
... The models of adenovirus replication as a linear molecule fail to provide a mechanism for the synthesis of the 5' ends of the DNA. If RNA is a primer in this system, there would not be a[r] ...
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Termination sites for adenovirus type 2 DNA replication.
... The fragment order (11) is shown on top of the figure. Fragments HpaI-A and HpaI-B were not separated.. Relative amounts of [3HJthymidi different fragments obtained by cleavage completed[r] ...
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Location of the Origin of DNA Replication in Adenovirus Type 2
... If one harvests the heavy side of the Ad2+ND, GC-rich half molecules after cesium chloride equilibrium centrifuga- tion and the light half of the Ad2+ND, AT-rich molecules, it can be sho[r] ...
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Adenovirus type 2 DNA replication. I. Evidence for discontinuous DNA synthesis.
... Analysis by alkaline sucrose gradient cen- trifugation of viral DNA labeled in Ad2-infected HeLa cells in the presence of HU. (Upper panel) Sedimenta-[r] ...
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DNA-Minus Temperature-Sensitive Mutants of Adenovirus Type 5 Help Adenovirus-Associated Virus Replication
... Thermolabile DNA-binding proteins from cells infected with a temperature-sensitive mu- tant of adenovirus defective in viral DNA synthesis. A quantitative[r] ...
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Intermediate in adenovirus type 2 replication.
... (B) Radioactivity in the peak fractions of the cesium chloride-ethidium bromide gradients was normalized for recovery of HeLa DNA in each gradient and plotted as a function of the time o[r] ...
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Adenovirus types 2 and 5 functions elicit replication and late expression of adenovirus type 12 DNA in hamster cells.
... The patterns of viral DNA integration have been published previously for the Ad2- transformed cell lines (45), with the exception of cell line HE7, as well as for the AdS-transformed ham[r] ...
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Role of the Adenovirus DNA-Binding Protein in In Vitro Adeno-Associated Virus DNA Replication
... short replication products characteristic of displacement of the elongating strand from the ...that replication of short substrate mol- ecules in extracts from uninfected cells gave almost as much ...
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Adenovirus type 40 and 41 growth in vitro: host range diversity reflected by differences in patterns of DNA replication.
... In contrast to the above results, which show that the Ad4l strain produced more DNA, a different relationship was noted between Ad4O and Ad4l DNA synthesis in the nonpermissive fibroblas[r] ...
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Interference with simian virus 40 DNA replication by adenovirus type 2 during mixed infection of monkey cells.
... Genetic evidence for SV40 gene func- tion in enhancement of replication of human adenovirus in simian cells. Block to[r] ...
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Replication-Defective Bovine Adenovirus Type 3 as an Expression Vector
... However, several transformed foci were evident following the transfection of FBRCs with a plasmid, pTG4671, containing the E1 region of HAV-5 under the control of the mouse PGK promoter. The resulting transformed cells ...
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Requirement of Sur2 for Efficient Replication of Mouse Adenovirus Type 1
... The molecular mechanisms of Sur2 function in MAV-1 in- fection have not been fully investigated. It has been proposed that the primary mechanism by which E1A recruits the Medi- ator complex to transactivate the ...
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Studies on the Mechanism of Replication of Adenovirus DNA II. The Nature of Single-Stranded DNA in Replicative Intermediates
... Hybridization experiments of the single- stranded DNA with isolated complementary strands of adenovirus type 5 DNA showed that this DNA hybridized only with the viral L-strand (the stran[r] ...
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Initiation of adenovirus DNA replication.
... Adenovirus DNA was synthesized in vitro in the presence of [3H]dTTP (-4*) either with protein-free viral DNA (A) or with the DNA-protein complex as the template (B, C, and D). Samples we[r] ...
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Mechanisms of adenovirus DNA replication
... of adenovirus have been found to infect humans causing acute respiratory, gastrointestinal, ocular and urinary ...of adenovirus infections in man are seldom associated with fatal diseases, (the exception ...
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