Air–liquid interface
Impacts of cellulase deactivation at the moving air–liquid interface on cellulose conversions at low enzyme loadings
... account for the drop in cellulose hydrolysis over time. But Ooshima, Sakata, and Harano still suggested that endo–exo synergism was affected by agitation in their 1985 paper [9] and that surfactant addition [10] also ...
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Exposure of silver-nanoparticles and silver-ions to lung cells in vitro at the air-liquid interface
... Background: Due to its antibacterial properties, silver (Ag) has been used in more consumer products than any other nanomaterial so far. Despite the promising advantages posed by using Ag-nanoparticles (NPs), their ...
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A dose-controlled system for air-liquid interface cell exposure and application to zinc oxide nanoparticles
... the air-liq- uid interface and not under fully immersed (submerged) ...the liquid or be lost to the lateral walls of the cell culture vessel, which alters the dose of nanoparticles interacting with ...
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Air-Liquid Interface Method To Study Epstein-Barr Virus Pathogenesis in Nasopharyngeal Epithelial Cells
... ABSTRACT Epstein-Barr virus (EBV) is a ubiquitous gammaherpesvirus that estab- lishes a latent reservoir in peripheral B-lymphocytes with sporadic reactivation. EBV also infects epithelial cells, predominantly resulting ...
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Development and characterization of a polarized human endometrial cell epithelia in an air–liquid interface state
... Fig. 4 The expression of endometrial epithelial marker in air – liquid interface (ALI) cultured cells. The passage 1 (P1) endometrial epithelial cells cultured in an ALI state for 2 weeks (A) or in a ...
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Perfusion culture maintained with an air-liquid interface to stimulate epithelial cell organization in renal organoids in vitro
... an air-liquid interface condition [23], and thus the tubule structure in the renal organoids could not be maintained under submerged ...an air-liquid ...
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In vitro exposure of a 3D-tetraculture representative for the alveolar barrier at the air-liquid interface to silver particles and nanowires
... Despite the pulmonary retention and systemic distri- bution of spherical AgNMs, the majority of in vivo stud- ies reported no or only mild effects involving chronic pulmonary inflammation and abnormalities in the liver ...
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Gene expression of bovine embryos developing at the air-liquid interface on oviductal epithelial cells (ALI-BOEC)
... functionally [2, 3]. We recently developed an air-liquid interface (ALI) culture system for the long-term culture of differentiated bovine oviductal epithelial cells (ALI-BOEC) [4]. The ALI-BOEC ...
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Air-liquid interface exposure to aerosols of poorly soluble nanomaterials induces different biological activation levels compared to exposure to suspensions
... All the data was expressed as mean ± standard deviation (SD) from three independent experiments performed in triplicates. The statistical analysis was performed using Graphpad Prism 5.0 (GraphPad Software Inc., San ...
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Growth of human bronchial epithelial cells at an air-liquid interface alters the response to particle exposure
... Results: Following exposure of submerged NHBE cells to ambient air pollution particle collected in Chapel Hill, NC, RNA for IL-8, IL-6, heme oxygenase 1 (HOX1) and cyclooxygenase 2 (COX2) increased. The same cells ...
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Aerosol generation and characterization of multi-walled carbon nanotubes exposed to cells cultured at the air-liquid interface
... inhaled air, while being nourished with medium from the basolateral ...larger interface between airborne components and target cells as compared to the intermittent expos- ure or submerged exposure ...the ...
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Differentiated transplant derived airway epithelial cell cytokine secretion is not regulated by cyclosporine
... We have previously described these methods [26]. To demonstrate cell differentiation in air liquid interface culture, immunofluoresence labeling and confocal microscopy were utilized. Epithelial ...
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ajplung.00020.2019.pdf
... at air-liquid interface (ALI) was generated using a custom manifold sealed to each transwell (culture medium on the basolateral side) attached to a heated and humidified rodent ventilator in a 37°C, ...
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Analysis of anti-microbial and anti-biofilm activity of hand washes and sanitizers against S. aureus and P. aeruginosa
... using air-liquid interface tube method, and air-liquid interface cover slip ...sing air-liquid inte r f a ce cov er s lip ass ...
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TNF-α and IL-1β-activated human mesenchymal stromal cells increase airway epithelial wound healing in vitro via activation of the epidermal growth factor receptor
... the air-liquid interface (ALI-PBEC), and subsequently incubated with MSC conditioned medium (MSC-CM) that was generated in presence or absence of TNF- α /IL-1 β ...
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Airway epithelial homeostasis and planar cell polarity signaling depend on multiciliated cell differentiation
... MCCs regulate PCP signaling in the airway epithelium. The hallmark of PCP signaling in airway epithelia is the localization of core PCP proteins, such as Vangl1, to proximal or distal (Vangl1 is distally localized) side ...
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Adaptive Divergence in Experimental Populations of Pseudomonas fluorescens. V. Insight into the Niche Specialist Fuzzy Spreader Compels Revision of the Model Pseudomonas Radiation
... Phages are important determinants of microbial evolution and population structure (reviewed in Stern and Sorek 2010; Koonin and Wolf 2012). Their success in the face of asymmetry in the coevolutionary potential of ...
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β2-agonists promote host defense against bacterial infection in primary human bronchial epithelial cells
... to air-liquid interface (ALI), and treated with or without IL-13 (10 ng/ml) and/or 20% cigarette smoke extract (CSE) to mimic the airways of asthmatics and smokers with or without ...
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A 3D epithelial–mesenchymal co-culture model of human bronchial tissue recapitulates multiple features of airway tissue remodeling by TGF-β1 treatment
... In this study, we investigated the effect of TGF-β1 on human bronchial tissue remodeling using our original 3D co-culture model. We applied human bronchial epithelial cells (HBECs) onto the 3D collagen matrices, and ...
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<p>Nicotine-Free e-Cigarette Vapor Exposure Stimulates IL6 and Mucin Production in Human Primary Small Airway Epithelial Cells</p>
... Small airway (<2 mm diameter) epithelial cells (SAEC) were collected from cut pieces of the distal lung using a 2 mm bronchoscopy brush (Conmed Greenwood Village, CO). The fl exible bristles of the brush allowed ...
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