Akt/eNOS
Original Article Silent information regulator 1 ( SIRT1 ) promotes the migration and proliferation of endothelial progenitor cells through the PI3K/Akt/eNOS signaling pathway
... different eNOS agonists, such as VEGF, insulin, estrogen, and platelet-derived lipid mediators, can affect eNOS activity through the PI3K/AKT ...specific eNOS/NO pathway were closely ...
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<p>Hydrogen Sulfide Protects Against High Glucose-Induced Human Umbilical Vein Endothelial Cell Injury Through Activating PI3K/Akt/eNOS Pathway</p>
... from Gibco-BRL (Grand Island, NY, USA). Fetal bovine serum (FBS), 2 ′ , 7 ′ -dichloro fl uorescein diacetate (DCFH- DA), 740 Y-P (a PI3K agonist), LY294002 (a reversible PI3K inhibitor), sodium dodecyl ...
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Original Article Exendin-4 attenuates high glucose-induced endothelial progenitor cells dysfunction through the PI3-K/Akt/eNOS pathway
... of eNOS. As shown in Figure 3B, the phosphorylation of eNOS was decreased in response to high glucose stimulation, but treat- ment with exendin-4 significantly up-regulated the phosphorylation of ...of ...
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Exosomes derived from atorvastatin-pretreated MSC accelerate diabetic wound repair by enhancing angiogenesis via AKT/eNOS pathway
... the AKT/eNOS pathway has been proved to stimulate several crucial processes of angio- genesis, including endothelial cell survival, migration, and tube formation of endothelial cells ...the ...
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κ-Opioid receptor stimulation reduces palmitate-induced apoptosis via Akt/eNOS signaling pathway
... Caspases, a group of vastly well-preserved, cysteine- dependent and aspartate-specific proteases, play a vital role in the regulation and execution of apoptosis. It was wildly accepted as signal of apoptosis. There are ...
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Original Article Overexpression of PER2 inhibits the progression of atherosclerosis via the Akt-eNOS signaling in apolipoprotein e-null mice
... 33]. eNOS, the mainly gen- erator of NO, catalyzes conversion of L-arginine to L-citrulline and NO ...[34]. Akt, a serine-threo- nine kinase, regulates several biological pro- cesses in protection of ...
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Ischemic postconditioning attenuates liver warm ischemia-reperfusion injury through Akt-eNOS-NO-HIF pathway
... NO, eNOS, SOD, p-Akt and HIF-1a, and decrease in ROS, TNF-a and ...of Akt and inhibition of ...through Akt-eNOS-NO-HIF path- way for the better management of liver warm I/R ...
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MicroRNA-130a alleviates human coronary artery endothelial cell injury and inflammatory responses by targeting PTEN via activating PI3K/Akt/eNOS signaling pathway
... while Akt, a downstream serine-threonine kinase, transmits survival signals from growth ...that Akt can activate eNOS, which can cause the production of NO ...of AKT, which can activate its ...
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Original Article Uric acid promotes cardiomyocyte apoptosis through regulating microRNA-21/PTEN/AKT/eNOS axis in myocardial ischemia/reperfusion injury
... predicted target sites of miR-21 or a mutated sequence with the predicted target sites to downstream of the pGL3 luciferase reporter gene and employed Western blotting assay. When wild-type (wt) or mutant vector was ...
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Osteocalcin attenuates high fat diet-induced impairment of endothelium-dependent relaxation through Akt/eNOS-dependent pathway
... Rabbit anti-mouse phosphorylated (P)-Akt (Ser473) (1:1000 dilution), total-Akt (1:1000), P-endothelial nitric oxide synthase (eNOS; Ser1177) (1:1000), total-eNOS (1:1000), P-PI3K (1:1000), ...
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A novel role for small molecule glycomimetics in the protection against lipid induced endothelial dysfunction : Involvement of Akt/eNOS and Nrf2/ARE signaling
... HUVECs were lysed in RIPA buffer, containing proteinase inhibitors and total protein quantified using BCA protein assay kit (Pierce Biotechnology). Proteins (30 μg/well) were denatured and separated by sodium dodecyl ...
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Original Article Vaspin alleviates dysfunction of endothelial progenitor cells induced by high glucose via PI3K/Akt/eNOS pathway
... decreased eNOS, FoxO1, and Akt phosphorylation, which inhibit the bioavailability of NO leading to reduce the number and migration capacity of ...by eNOS inhibitor L ...
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<p>(-)-Epigallocatechin-3-Gallate Inhibits eNOS Uncoupling and Alleviates High Glucose-Induced Dysfunction and Apoptosis of Human Umbilical Vein Endothelial Cells by PI3K/AKT/eNOS Pathway</p>
... (-)-Epigallocatechin-3-gallate (EGCG), as the most effective component in green tea, is recognized as a strong antioxidant, which can effectively scavenge free radicals and reduce the formation of ROS by chelating metal ...
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Wen Xin Decoction ameliorates vascular endothelium dysfunction via the PI3K/AKT/eNOS pathway in experimental atherosclerosis in rats
... the eNOS activity can be regulated at both protein expression [16] and gene transcription levels, including eNOS phosphorylation and the inter- actions of other active substances [17, ...enhance eNOS ...
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sMEK1 inhibits endothelial cell proliferation by attenuating VEGFR-2-dependent-Akt/eNOS/HIF-1α signaling pathways
... Specific pathogen-free BALB/c-nu/nu mice (5~6 week old) were supplied by Orientbio (Sungnam, Korea). All animal studies were approved by the Institutional Animal Care and Use Committee (IACUC) at the Research Institute ...
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Original Article PI3K/AKT signaling pathway plays a role in enhancement of eNOS activity by recombinant human angiotensin converting enzyme 2 in human umbilical vein endothelial cells
... the Enos mRNA cell level and non- phosphorylated eNOS protein levels in each group do not increase, rhACE2 can significantly increase the release of endothelial ...increases eNOS activity through ...
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Reverse-D-4F improves endothelial progenitor cell function and attenuates LPS-induced acute lung injury
... High-density lipoprotein (HDL) regulates EPC mobilization from bone marrow, prevents EPC apoptosis, and pro- motes differentiation of EPC into endothelial cells [7]. Apolipoprotein A-I (apoA-I), an important functional ...
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Molecular mechanisms of hydrogen sulfide against uremic accelerated atherosclerosis through cPKCβII/Akt signal pathway
... cPKCβII, Akt, phosphorylated-Akt (p-Akt), eNOS of total protein in mice aorta were analyzed by Western ...Cruz), eNOS (goat anti-rabbit, 1:5000 dilution, Santa ...
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... of Akt, eNOS, and ...of eNOS, but AMPK was activated significantly later than eNOS in this ...activated Akt, which promoted the increased phosphorylation of its downstream signaling ...
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Structural and functional abnormalities of penile cavernous endothelial cells result in erectile dysfunction at experimental autoimmune prostatitis rat
... by eNOS is an important factor in penile erec- tion, which involved Akt-eNOS-cGMP pathway ...reported eNOS was decreased significantly both in EAP rat corpus cavernosum and corpus cavern- osum ...
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