Analysis of Samples from Virus-Infected Cells
Analysis of cytoplasmic RNA and polyribosmomes from feline leukemia virus-infected cells.
... periments with the R-FeLV cDNA were per- formed across polyribosome gradients to corre- late the binding of anti-FeLV to nascent virus- specific protein RNA within polyribosomes. The R-F[r] ...
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Analysis of proteins synthesized in respiratory syncytial virus-infected cells.
... tein Vp86 was the major protein detected in RS virus-infected cell extracts of LLC-MK2 and. CV-1 cells when a [3H]glucosamine label was[r] ...
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Quantitative Whole-Cell Proteome Analysis of Pseudorabies Virus-Infected Cells
... kidney cells after infection with the alphaherpesvirus pseudorabies virus (PrV), the etiological agent of Aujeszky’s ...for virus-induced changes in relative protein ...
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Analysis of the In Vitro Product of an RNA-Dependent RNA Polymerase Isolated from Influenza Virus-Infected Cells
... Approximately 50% of the RNA synthesized in vitro remains associated with the 50 to 70S ribonucleoprotein complex containing polymerase activity; the remainder of the RNA polymerase prod[r] ...
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Analysis of the Fusion of XC Cells Induced by Homogenates of Murine Leukemia Virus-Infected Cells and by Purified Murine Leukemia Virus
... The results of this study show that intact, infected cells are not required, but that homogenates of the in- fected cells and also purified viral particles will induce XC cell fusion. Mu[r] ...
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Analysis of HCF, the Cellular Cofactor of VP16, in Herpes Simplex Virus-Infected Cells
... in virus-infected cells, our results suggest that the mech- anism responsible for the localization of HCF late in infected cells and in particular its presence in viral replication ...
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Global transcriptome analysis of H5N1 influenza virus-infected human cells
... A549 cells, we found that the most enriched biological pro- cesses mainly include the oxidation-reduction process, defensive response to viral invasion, and the signaling pathway mediated by type I interferon ...
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Small RNA analysis in Sindbis virus infected human HEK293 cells
... the analysis of the whole SINV ...for samples 4 hpi and 6 hpi were statistically the same as for the 0 hpi sample, suggesting no influence of the virus infection to modulate the miRNA expression ...
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Dissemination of Bovine Leukemia Virus-Infected Cells from a Newly Infected Sheep Lymph Node
... when virus-bearing cells first become evident in blood ...fected cells increase sharply, antibodies specific for the SU glycoprotein become detectable in ...lymph samples from ...
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Analysis of herpes simplex virus nucleoprotein complexes extracted from infected cells.
... The cells were labeled from 5 to 18 h after infection with [3H]thymidine, and then extraction, sucrose gradient purification, sample deproteinization, and CsCl centrifugation were perfor[r] ...
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Role of pseudorabies virus glycoprotein gI in virus release from infected cells.
... That deletion of gI affects both virus release and plaque size of the rescued Bartha virus, but does not affect PrV(Ka) in the same way, indicates that the effect of gI on virus release [r] ...
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Shedding of the glycoprotein from vesicular stomatitis virus-infected cells.
... and G proteins of vesicular stomatitis virus suggest that the Gs protein is derived from the G protein by proteolysis.. Both proteins are hydrophobic when analyzed by charge-shift electr[r] ...
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Specific secretion of polypeptides from cells infected with vaccinia virus.
... Order of the samples: A, uninfected cell profile after a 30-min pulse; B, uninfected cell profile after a 30-min pulse in the presence of CAR following treatment for 5 h with 25 pg of CA[r] ...
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Hepatitis C Virus Infection Is Blocked by HMGB1 Released from Virus-Infected Cells
... knockdown cells by treating wild-type HMGB1 for 48 ...transfected cells was reduced by 63 and 39%, respectively, com- pared to that of the control siRNA-transfected ...HCV-infected cells and ...
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Temperature-Sensitive Virus from Aedes albopictus Cells Chronically Infected with Sindbis Virus
... Similarly, small plaque variants became predominant in cultures of Aedes aegypti cells chronically infected with Semliki forest virus (12) and in Aedes albopictus cells chronically infec[r] ...
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A Virus-Encoded RNA Polymerase Purified from Baculovirus-Infected Cells
... fever virus are the only other examples of eukary- otic viruses that encode DNA-dependent RNA polymerases, and their need for a virus-encoded enzyme is usually attributed to the fact that they replicate in ...
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Isolation and characterization of cytoplasmic inclusions from influenza A virus-infected cells.
... Purified inclusions isolated from infected BHK-21-F cells by fluorocarbon extraction and gradient fractionation, negatively stained with uranyl acetate. The high-magnification inset show[r] ...
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Infective substructures of measles virus from acutely and persistently infected cells.
... off35S]methionine- labeled polypeptides obtained from cytoplasmic extracts before sucrose gradient fraction ation: track a, measles virus-infected cells; track b, mock-infected cells. Po[r] ...
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Characterization of polysome-associated RNA from influenza virus-infected cells.
... To assure saturating conditions, increasing concentrations (1 x, 2 x, 3 x, etc.) of unlabeled RNA species (either total cell RNA containing cRNA prepared as above, or unlabeled vRNA extr[r] ...
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Isolation and Properties of an RNA Polymerase from Influenza Virus-Infected Cells
... polymerase activity in fraction 4 from influenza virus-infected BHK21-F cells.. Batches of about 3 X 108 cells were harvested at each time point, and fractions were assayed for RNA polym[r] ...
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