Aqueous Two-Phase Systems
Characterization of Copolymer Dehypon® LS 54 and Its Application for Aqueous Two-Phase Systems Paired with the Waxy Maize Starch for Protein Extraction
... composition. Aqueous two-phase systems were prepared with a final mass of 10 ...the phase systems had been mixed thoroughly in a closed test tube by a vortex mixer, phase ...
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Partitioning of Cholesterol Oxidase Produced by Streptomyces olivaceus MTCC 6820 in Different PEG-Salt-Water Aqueous Two Phase Systems
... of aqueous two phase systems (ATPS) for partitioning of cholesterol oxidase (Chox) produced by Streptomyces olivaceus MTCC 6820 was done using eight different ATPS composed of two ...
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Aqueous Two Phase Systems Applied to Partition Proteins from Goat Milk Whey In Nature
... The aqueous two-phase systems (ATS) have been utilized with success in the partition of these proteins, however, the studies were performed using protein in its pure ...of aqueous ...
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Rapid One-Step Separation and Purification of Recombinant Phenylalanine Dehydrogenase in Aqueous Two-Phase Systems
... performs the reversible oxidative deamination of L-phenylalanine to phenylpyruvate. It plays an important role in detection and screening of phenylketonuria (PKU) diseases and production of chiral intermediates as well. ...
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Recovery of Biological Products in Aqueous Two Phase Systems - Volume 1 Number 2 (Aug. 2010) - IJCEA
... bio-products. Aqueous two phase systems (ATPSs) have overcome these demands and emerged as a powerful method for the downstream processing of ...the two phases including polymer ...
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A Review on Aqueous Two Phase Systems with a Special Mention on Application of Ionic Liquids
... Aqueous two-phase systems (ATPS) are formed when two polymers or polymer/ salt system are mixed above a critical ...this aqueous environment provides stability, solubility and ...
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Comparison between Polyvinyl Pyrrolidone/Na2SO4 Aqueous Two-Phase Systems and Chromatographic Methods for Purification of Recombinant Phenylalanine Dehydrogenase
... Phenylalanine dehydrogenase (PheDH; EC 1.4.1.20) is an important enzyme of amino acid dehydrogenases family that increasingly used as a valuable biocatalyst in neonatal screening kits and synthesis of L-phenylalanine. ...
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Separation/Analysis Rhodamine B by Anion Surfactant/Ionic Liquid Aqueous Two Phase Systems Coupled with Ultraviolet Spectrometry
... The aqueous two-phase systems (ATPSs) are a kind of LLE technique which is a simple and environmentally friendly separation system because traditional volatile solvents are not used in the ...
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Partitioning behavior of methylene blue in aqueous two phase systems
... Aqueous two phase systems (ATPS) have been considered an attractive method to extract different compounds such as metals [1,2], biomolecules [3,4], and dyes ...mixing aqueous solutions ...
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Interfacial Assembly In Aqueous Two Phase Systems
... of systems that has shown great promise in recapitulating some of the features found in living cells is aqueous two phase systems (ATPSs), comprising mixtures of two water ...
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Polymerization of a new thermo-responsive copolymer with N-vinylcaprolactam and its application in recyclable aqueous two-phase systems with another thermo-responsive polymer
... Aqueous two-phase systems (ATPS) are promising alter- natives to extract and purify biomolecules thanks to high biocompatibility and readiness to scaling up (Molino et ...
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Recombinant d-galactose dehydrogenase partitioning in aqueous two-phase systems: effect of pH and concentration of PEG and ammonium sulfate
... NAD + binding first to the enzyme. The substrates of GalDH are D-galactose and NAD + , whereas its products are D-galactono-1,4-lactone, NADH, and H + [5]. GalDH has been identified in plants (e.g., green peas and ...
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Affinity Partitioning in Aqueous Two Phase Systems pdf
... Af R nity membrane separation techniques com- bine the speci R city of af R nity adsorption with the unique hydrodynamic characteristics of porous membranes. They provide low pressure separation systems which are ...
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Current applications of different type of aqueous two-phase systems
... an aqueous solution above the cloud point (lower critical solution temperature, LCST), the lin- ear and non-ionic EOPO random copolymers will be thermo-separated into two phases from aqueous solu- ...
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Fluoroalcohol Mediated Aqueous Two-Phase Systems: Characterization and Applications in Organic Synthesis and Extraction
... stationary phase which is one of the main variables in improving extraction ...stationary phase does necessitate the use of longer equilibration ...stationary phase either during extraction or ...
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Purification and characterizations of a novel recombinant Bacillus velezensis endoglucanase by aqueous two-phase system
... Aqueous two-phase systems were prepared at room tem- perature by mixing certain amounts of 50% (w/w) of PEG solution, 40% (w/w) of phosphate buffer, 2 ml cell culture supernatant sonicated in ...
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Central composite design application for optimization of aqueous two phase extraction of protein from shrimp waste
... An experimental design was used to optimize protein partitioning from shrimp waste using Polyethylene glycol/sodium citrate aqueous two-phase systems (ATPS). Four factors like PEG’s molecular ...
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Quantification of amino acids and peptides in an ionic liquid based aqueous two-phase system by LC–MS analysis
... Aqueous two-phase systems (ATPS) occur by the mixture of two polymers or a polymer and an inorganic salt in ...ATPS systems are hydrophilic water miscible ionic ...liquids, ...
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Optimization Of Papain Extraction By Aqueous Two-Phase System (Atps) Using Response Surface Methodology (Rsm)
... Conventional techniques for separation and purification of biomaterials have some disadvantages such as insufficient selectivity, high energy consumption and possibility of denaturation of biomolecules when organic ...
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Potential Recovery of Protein from Shrimp Waste in Aqueous two Phase System
... C). Phase systems were prepared in 50 ml graduated centrifuge tubes by weighing out appropriate quantities of the PEG of desired molecular weight and sodium citrate stock solutions and added to crude shrimp ...
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