ARMS PCR
Comparison of TaqMan Real-Time and Tetra-Primer ARMS PCR Techniques for Genotyping of Rs 8066560 Variant in Children and Adolescents with Metabolic Syndrome
... Tetra-primer ARMS–PCR is a rapid, simple, robust, low-cost, and easy to use method for SNP genotyping [24] which differs from its classical version in such a way that it does not involve two separate ...
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Tetra Primer ARMS PCR Optimization to Detect Single Nucleotide Polymorphism of the KLF14 Gene
... T-ARMS PCR assay to genotype ...T-ARMS PCR were con- cordant with sequencing. T-ARMS PCR assay developed in our laboratory for genotyping rs972283 (A/G) of KLF14 gene is time ...
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ARMS-PCR Based SNP Analysis of MTHFR C677T Allele Using Syber Green in Pancreatic Tumour
... Pancreatic tumour, a most common form of cancer mortality and their incidence varies in different countries due to different life style including food habit and other environmental factors [1,2]. To reduce the global ...
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T ARMS PCR genotyping of SNP rs445709131 using thermostable strand displacement polymerase
... T-ARMS PCR genotyping (Additional file 1: Figure ...complex PCR with Taq polymerase need more optimization for the cor- rect genotype pattern as shown in case of T-ARMS PCR for the ...
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Sensitive detection of BRAF V600E mutation by Amplification Refractory Mutation System (ARMS)-PCR
... Using ARMS-PCR, we tested 72 thyroid tumors and determined that the frequencies of BRAF V600E mutation in the conventional, tall-cell and follicular variants of PTC were 66%, 75% and 0%, respect- ...by ...
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Detection of Rare Mutations in EGFR ARMS PCR Negative Lung Adenocarcinoma by Sanger Sequencing
... 100 ARMS- positive EGFR samples, Sanger sequencing detected muta- tions in 90 samples; the other 10 were ...100 ARMS-negative samples, three were positive for a mutation by the Sanger method, and 97 ...
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Multiplex Amplification Refractory Mutation System Polymerase Chain Reaction (ARMS PCR) for diagnosis of natural infection with canine distemper virus
... multiplex ARMS-PCR. (A) Results of a multiplex PCR us- ing the two primer pairs: F-vacc/R-vacc and ...Vacc-P PCR product with BamH I enzyme re- sulted in a smaller DNA fragment (~500 bp; lane ...
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Evaluation of multiplex ARMS-PCR for detection of Helicobacter pylori mutations conferring resistance to clarithromycin and levofloxacin
... Apart from the high precision of the test established in validation and clinical studies, the multiplex ARMS- PCR assay we developed and validated has considerable additional advantages for the analysis of ...
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Original Article Simple and reliable genotyping of ALDH2 rs671 in the patients with acute alcoholism intoxication using Tetra-Primer ARMS PCR
... Tetra-Primer ARMS PCR, a serial dilution (ranging from 100 ng to 5 ng) of the P1 DNA was used as the PCR template (Figure ...Tetra-Primer ARMS PCR for the genotyping of ALDH2 rs671 was ...
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TETRA PRIMER ARMS PCR DETECTED A MISSENSE MUTATION OF BRCA1 AND BRCA2 IN BREAST CANCER PATIENTS IN JEDDAH CONTROL STUDY
... Genomic DNA was extracted using ReliaPrepTM Blood gDNAMiniprep System kits (PROMIGA, USA); it was taken from whole blood and stored in EDTA coated tubes (Lavender top tube, Franklin Lakes, NJ, USA). Amplification for ...
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A comparison of QuantStudio™ 3D Digital PCR and ARMS-PCR for measuring plasma EGFR T790M mutations of NSCLC patients
... Digital PCR assay to detect plasma EGFR T790M, we compared the result of ctDNA extracted from one plasma sample detected by our assay with the CFDA-approved ADx-ARMS EGFR mutation ...do ...
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RFLP-PCR is more efficient than ARMS-PCR for identifying CYP2C19*2 polymorphism in atherosclerotic patients
... The ARMS-PCR system is performed by combining two allele-specific primers and two common external ...the ARMS system, the design of compatible primers for simultaneous amplification is often ...
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TRANSFORMING GROWTH FACTOR-Β1 T869C (CODON 10) GENE POLYMORPHISM IN TYPE 2 DIABETES IRAQI PATIENTS
... primer-ARMS PCR technique which developed in our study an effective, robust assay and time saving for genotyping (T/C) of TGFβ1 gene, Our results were concluded TGFβ1T869C (codon 10) C allele, and C ...
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Multimethod Longitudinal HIV Drug Resistance Analysis in Antiretroviral Therapy Naive Patients
... of ARMS-PCR with NGS and Sanger ...sequencing, ARMS-PCR performed very well, with 100% sensitivity based on no false-negative determinations for all studied ...
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Glucose 6 phosphate dehydrogenase deficiency and hemoglobinopathy in South Western Region Nepal: a boon or burden
... This community based cross sectional study was car- ried out in total of 100 suspected cases of South West- ern Region, province-5, Nepal. Districts with dominance of tharu ethnicity were chosen viz a viz Nawalparasi, ...
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Use of Amplification Refractory Mutation System PCR Assay as a Simple and Effective Tool To Detect HIV 1 Drug Resistance Mutations
... The ARMS-PCR assay was able to detect M184V, T215Y/F, K103N, and Y181C mutations with sensitivities of ...(97.5%). ARMS-PCR’s limits of detection for mutations M184V, T215Y/F, K103N, and Y181C were ...
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Efficacy and safety of icotinib in treating non-small cell lung cancer: a systematic evaluation and meta- analysis based on 15 studies
... effects, ARMS-PCR : amplification refractory mutation system, CFDA : China Food and Drug Administration, DCR : disease control rate, DNA : deoxyribonucleic acid, CI : confidence interval, EGFR : epidermal ...
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Polymer nanofiber-based microchips for EGFR mutation analysis of circulating tumor cells in lung adenocarcinoma
... of ARMS-PCR to those of CT images, T790M mutation could be detected long before tumor progression, which means that CTC analysis can provide possibility for early ...
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Development of a high resolution melting analysis assay for rapid identification of JAK2 V617F missense mutation and its validation
... method, ARMS-PCR depend on primers and the set up performance, PCR–RFLP depend on the enzyme and the control sequence and the HRM depend on the instrument and the ability of the laboratory to set up ...
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Evaluation of amplification refractory mutation system (ARMS) technique for quick and accurate prenatal gene diagnosis of <em>CHM</em> variant in choroideremia
... evaluate ARMS for quick and accurate prenatal gene diagnosis of CHM, ARMS- PCR was performed and results are shown in Figure 4A, which indicates that the fetus (M151) amplified both mutant as well as ...
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