b Loop mediated isothermal amplification
Loop mediated isothermal amplification for the detection of goose circovirus
... describes loop-mediated isothermal assay (LAMP) that allows simple, sensitive and specific detection of goose circovirus DNA with naked eye or under UV-light as soon as 30 min using only waterbath or ...
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Loop Mediated Isothermal Amplification for Detection of African Trypanosomes
... an isothermal reaction for a rather short time (⬇1 h) is enough to amplify the target DNA to detectable ...DNA amplification method in many places, under field conditions and in local clinics and hospitals ...
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A Designed Enclosure Unit for Loop-Mediated Isothermal Amplification
... gene amplification technique is used in medical science and biotechnology ...The loop-mediated isothermal amplification (LAMP) is a nucleic acid amplification method that can be ...
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Validation of loop-mediated isothermal amplification (LAMP) for fast and
... also designed specific primers to be used as positive control for Falconiformes (primer 184.. set FAL-Z) based on CHD-Z sequences of all raptor species found in GenBank (see 185.. Supp[r] ...
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Loop-mediated isothermal amplification assays for screening of bacterial integrons
... no amplification was obtained in the absence of each of FIP, BIP, F3 or B3 primers, demonstrating LAMP assays were applicable only in the existence of both inner and outer ...the loop out ...
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Detection of Pathogen-Specific Antibodies by Loop-Mediated Isothermal Amplification
... a visual signal is developed by supplementation with primers and Bst polymerase. The inclusion of overlapping amplicons in the DNA element of the LAMPole enables the use of two separate LAMP primer sets to generate ...
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Loop Mediated Isothermal Amplification Assay for Detection of Haemophilus influenzae Type b in Cerebrospinal Fluid
... type b (Hib) is one of the leading causes of meningitis in developing ...novel loop-mediated isothermal amplification (LAMP) assay for Hib, we designed a LAMP primer set targeting the ...
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Loop mediated isothermal amplification (LAMP) shield for Arduino DNA detection
... The Arduino code, in a few words, controls the ther- mistor and heats the coil until it reaches 65 °C, or any other temperature set by the user in the variable TAR- GET_TEMP, and it keeps the set temperature by switch- ...
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Wolbachia detection in insects through LAMP: loop mediated isothermal amplification
... 16. Goto M, Honda E, Ogura A, Nomoto A, Hanaki K-I: Colorimetric detection of loop-mediated isothermal amplification reaction by using hydroxy naphthol blue. Biotechniques 2009, 46:167 – 172. ...
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Loop mediated isothermal amplification assay for rapid detection of staphylococcus aureus
... of loop primers indicating that it was not mandatory to include loop primers in the ...under isothermal condition; time loss due to thermal changes could be ...
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Loop mediated isothermal amplification for detection of porcine circovirus type 2
... Principle of LAMP demonstrates that forming an initial stem-loop structure later, inner primers hybridize to regions of target sequences at the same time [13]. The final products are DNAs with different numbers of ...
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A loop mediated isothermal amplification assay for the visual detection of duck circovirus
... the amplification efficiency of the LAMP assay, which subsequently affects the fluorescence emission of the resulting ...the amplification reaction of DuCV LAMP assay can be ac- complished in a conventional ...
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The Development of a Loop Mediated Isothermal Amplification (LAMP) Procedure for Plague Diagnostic
... Considering the LAMP advantages, we developed a procedure for detecting the Y. pestis caf1 gene that was both specific and sensitive. At least 10 pg of Y. pestis DNA were be detected in parallel reactions by LAMP and ...
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Loop Mediated Isothermal Amplification for Rapid and Reliable Diagnosis of Tuberculous Meningitis
... Despite the encouraging results obtained using LAMP, there were some issues of major concern. Two of the clinically suspected TBM cases appeared to be LAMP negative. Despite confirmatory observations, this issue needs to ...
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Detection of Human Herpesvirus 7 DNA by Loop Mediated Isothermal Amplification
... of loop-mediated isothermal amplification (LAMP), initially developed for the detection of human herpesvirus 7 (HHV-7), was evaluated in this ...
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Loop Mediated Isothermal Amplification for Rapid Detection of Newcastle Disease Virus
... the loop-mediated isothermal amplification (LAMP) assay for the rapid, simple, and sensitive detection of Newcastle disease virus (NDV) directly from culture isolates as well as clinical ...
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Development of a Loop Mediated Isothermal Amplification Assay for Rapid Detection of Capripoxviruses
... Sheep pox (SP), goat pox (GP), and lumpy skin disease (LSD), caused by capripoxviruses (CaPVs), are economically important diseases of sheep, goats, and cattle, respectively. Here, we report the development of a ...
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The detection of Plasmodiophora brassicae using loop-mediated isothermal DNA amplification
... Plasmodiophora brassicae, the cause of clubroot, is a very serious problem preventing from successful and profitable cultivation of oilseed rape in Poland. The pathogen was found in all main growing areas of oilseed ...
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Development of loop mediated isothermal amplification (LAMP) for simple detection of Leishmania infection
... Chaichontat Sriworarat 1 , Atchara Phumee 2 , Mathirut Mungthin 3 , Saovanee Leelayoova 3 and Padet Siriyasatien 2,4* Abstract Background: Leishmaniasis is a neglected tropical disease that is caused by an obligate ...
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Ultrasensitive loop mediated isothermal amplification (US LAMP) to detect malaria for elimination
... Namangala B, Sugimoto ...transcription-loop-mediated isothermal amplification (RT-LAMP) for clinical detection of Plasmodium falciparum ...
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