Bacterial artificial chromosomes (BACs)
An easy and versatile 2 step protocol for targeted modification and subcloning of DNA from bacterial artificial chromosomes using non commercial plasmids
... Bacterial artificial chromosomes (BACs) are low copy plasmids based on the F-plasmid of ...made BACs an important source for the generation of transgenic organisms, because they carry ...
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Combined use of bacterial artificial chromosomes-on-beads with karyotype detection improves prenatal diagnosis
... the bacterial artificial chromosomes (BACs)-on-Beads (BoBs ™ ) technology for the detection of common aneu- ploidies and specific microdeletion syndromes [1, 9 – ...
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Oncogenicity of Virulent Marek's Disease Virus Cloned as Bacterial Artificial Chromosomes
... Bacterial artificial chromosomes (BAC) containing full- length genomes of several herpesviruses have enabled the ap- plication of rapid mutagenesis strategies to identify functions of individual ...
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Sequence Conservation of Homeologous Bacterial Artificial Chromosomes and Transcription of Homeologous Genes in Soybean (Glycine max L. Merr.)
... SSRs were identified from each BAC: 14 SSRs for gmw1-74i13 and 8 SSRs for gmw1-52d3. Genetic map- ping placed gmw1-74i13 on linkage group C1 and gmw1-52d3 on linkage group C2 of the G. max A81- 356022 3 Glycine soja PI ...
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High-Resolution Pachytene Chromosome Mapping of Bacterial Artificial Chromosomes Anchored by Genetic Markers Reveals the Centromere Location and the Distribution of Genetic Recombination Along Chromosome 10 of Rice
... pachytene chromosomes hybridized to BAC clones 45D16 (red) and 15O22 (green), which are separated by ⵑ137 ...pachytene chromosomes hybridized to BAC clones 82M15 (red) and E14I11 (green), which are ...
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A Molecular Cytogenetic Map of Sorghum Chromosome 1: Fluorescence in Situ Hybridization Analysis With Mapped Bacterial Artificial Chromosomes
... sorghum chromosomes with analogous gion accounts for just ⵑ4% of wheat chromosome 5B, multiprobe cocktails (Kim et ...sorghum BACs to rice and maize Determining the distribution of heterochromatin and ...
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Genetic Linkage Maps of the Red Flour Beetle, Tribolium castaneum, Based on Bacterial Artificial Chromosomes and Expressed Sequence Tags
... from bacterial artificial chromosome (BAC) ends and untranslated regions from random ...190 BACs and 165 cDNA’s, as well as 69 genes, transposon insertion sites, sequence- tagged sites, ...
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The construction and characteristics of a BAC library for Cucumis sativus L. ‘B10’
... using bacterial artificial chromosomes (BACs) can yield high quality genomic libraries, which are used for the physical mapping, identification and isolation of genes, and for gene ...
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Capture Hi C identifies a novel causal gene, IL20RA, in the pan autoimmune genetic susceptibility region 6q23
... of bacterial artificial chromosomes (BACs) (Life Technolo- gies) spanning the region of interest with minimal overlap (Additional file 1: Table ...
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Loss of SETDB1 decompacts the inactive X chromosome in part through reactivation of an enhancer in the IL1RAPL1 gene
... BAC: bacterial artificial chromosomes; ChIP-Seq: chromatin immunoprecipita- tion coupled with high-throughput sequencing; DHS: DNase I hypersensi- tive site; EZH2: enhancer of Zeste 2; FISH: ...
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Mitotic transmission of artificial chromosomes in cdc mutants of the yeast, Saccharomyces cerevisiae.
... Rates of 1:0 and 2:O events for a linear chromo- some fragment in cells reduced for cdc function: T o address whether small circular minichromosomes and large linear [r] ...
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Thirty years ago in Genetics: prophage insertion into bacterial chromosomes.
... Whereas classical linkage analysis and cyto- genetics provided definitive evidence that genes were linearly disposed along the length of the chromosome, and fine structure gen[r] ...
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The antimicrobial polymer PHMB enters cells and selectively condenses bacterial chromosomes
... above. Bacterial pellets were re-suspended in 1× PBS and 80 μl of the suspension was centrifuged in a Shandon Cytospin 2 cytocentrifuge at 800 rpm (75 × g) for 5 minutes before circumscribing the slide-deposited ...
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Integration of DNA into bacterial chromosomes from plasmids without a counter selection marker
... Figure 3. Multistep insertion of the chromosome of phage lambda into the chromosome of C. acetobutylicum. (A) Chromosome of phage lambda showing restriction sites used to excise the three overlapping fragments. Yellow, ...
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Comparison of Plant and Microbial Communities between an Artificial Restoration and a Natural Restoration Topsoil in Coal Mining Subsidence Area
... The bacterial community diversity and plant species in the artificial restoration area were higher. than those in the natural area[r] ...
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A low frequency persistent reservoir of a genomic island in a pathogen population ensures island survival and improves pathogen fitness in a susceptible host
... It can be seen from this simple linear analysis that the stability of S 1 and S 2 depends only upon the bacterial reproductive ratios ρ c and ρ R (of B c and R respectively) and the plant host’s carrying capacity ...
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The Impact of Organic Acids and pH on the Virulence Factor Expression of E. coli O157:H7.
... The production of Shiga toxins is one of the defining characteristics of EHEC as these toxins are thought to be responsible for the principal manifestations of HC and HUS (Law, 2000). However, the mechanism of ...
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A yeast artificial chromosome clone map of the Drosophila genome.
... We describe the mapping of 979 randomly selected large yeast artificial chromosome (YAC) clones of Drosophila DNA by in situ hybridization to polytene chromosomes.. Eight h[r] ...
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Gene duplication and fragmentation in the zebra finch major histocompatibility complex
... We estimated and compared gene density, across three avian lineages (chicken, quail and zebra finch) and the human MHC. To describe the human MHC, gene coor- dinates for protein coding genes were extracted from Ensembl ...
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A matheuristic approach to solve the multi objective beam angle optimisation problem in intensity modulated radiation therapy
... The BAO problem is usually stated as a mixed integer (or binary) optimisation problem (Bangert et al, 2012; Ehrgott and Johnston, 2003; Preciado-Walters et al, 2004, 2006), as the set of all possible angles K is discrete ...
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