Caco-2
Identification of Host Cell Factors Associated with Astrovirus Replication in Caco-2 Cells
... Astroviruses are small, nonenveloped viruses with a single-stranded positive-sense RNA genome causing acute gastroenteritis in children and immunocompromised patients. Since positive-sense RNA viruses have frequently ...
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Almond milk fermented with different potentially probiotic bacteria improves iron uptake by intestinal epithelial (Caco-2) cells
... Results of the possible toxicity of fermented sam- ples in intestinal epithelial (Caco-2) cells, which was quantified by monitoring the mitochondrial enzyme (MTT test) activities, are shown in Fig- ure ...
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APOPTOTIC EFFECT OF GOLD NANOPARTICLES ON HUMAN COLON CANCER CELL LINES CACO 2 BUT NOT ON THE NORMAL EPITHELIAL CELL LINES WISH
... SRB assay measure drug-induced cytotoxicity even at large-scale application. [21] Sulphorhodamine B (SRB) is a bright pink Aminoxanthine dye with two sulfonic groups. Under mild acidic conditions, SRB binds dye to basic ...
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Short communication: A study of Lactobacillus isolates' adherence to and influence on membrane integrity of human Caco-2 cells
... the Caco-2 cell monolayer, which reflects the intestinal barrier function of the epithelium cells, can be determined and measured in vitro in terms of trans-epithelial electrical resistance (TEER) of the ...
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Permeation of roxarsone and its metabolites increases caco 2 cell proliferation
... nized Caco-2 cells, the human colon adenocarcinoma cell line ...The Caco-2 cell line is a model frequently used for that purpose by the pharmaceutical industry ...[27-29]. Caco-2 ...
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In vitro toxicity of different sized ZnO nanoparticles in Caco 2 cells
... the Caco-2 monolayer is widely used across the pharmaceut- ical industry as an in vitro model of the human small intestine mucosa to predict the absorption of orally ad- ministered ...intestine. ...
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Influence of magnetic iron oxide nanoparticles on red blood cells and Caco 2 cells
... cells, Caco-2 cells) with magnetic iron oxide nanoparticles (non-grafted, citrate-grafted, dendrimer-grafted) of 11 nm in size have been ...Ca 2+ con- ...Ca 2+ con- tent of ...
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TMPRSS2 and TMPRSS4 Facilitate Trypsin-Independent Spread of Influenza Virus in Caco-2 Cells
... that Caco-2 cells indeed allow the trypsin-independent spread of influenza virus, as proposed by Zhirnov and coworkers ...in Caco-2 cells were fully infectious in the absence of trypsin ...
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Gene Expression Pattern in Caco-2 Cells following Rotavirus Infection
... in Caco-2 cells at 16 hpi, and the majority of these transcriptional responses correspond to upregulation of ...fected Caco-2 cells were harvested at 1, 6, 12, and 24 h after ...
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Differential effects of basolateral and apical iron supply on iron transport in Caco-2 cells
... The Caco-2 model of intestinal ep- ithelium was employed to compare effects of ferric ni- trilotriacetate (NTA), supplied to the apical surface of the cells to mimic dietary iron, or Tf-bound iron, supplied ...
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Quantitative analysis of lab to lab variability in Caco 2 permeability assays
... the Caco-2 assay [2- 8]. While the Caco-2 assay is the gold standard for in vitro permeability assessment, interpretation of the results from such assays needs careful attention due to ...
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Symmetric infection of rotavirus on polarized human intestinal epithelial (Caco-2) cells.
... Filter-grown Caco-2 cells and MDCK-1 cells, producing a transepithelial resistance of 300 to 500 and >1,000 fQ cm2, respectively, were infected from either the apical or basolateral doma[r] ...
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Evaluation of the Cytotoxicity of Cholesterol Oxides in Human Colon Cancer Caco 2 Cells
... in Caco-2 cells differently when administered ...undifferentiated Caco-2 cells in the range 0-62 μM cause a reduction in cell viability (measured with the neutral red uptake assay), following ...
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Caco-2 Cells, Biopharmaceutics Classification System (BCS) and Biowaiter
... as Caco-2 cells, with unusually high degree of differentiation together with the fact that these cells differentiate sponta- neously in normal serum-containing cell culture medium, has become one of the ...
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Dietary isothiocyanates as apoptotic inducer for human colorectal cancer (CACO 2) cells
... of Caco-2 cells and blocked the cell cycle as previously described (Kristal and Lamp, ...2002). Caco-2 cells which were treated with isothiocyanates exhibited increased levels of p53 ...
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Caco-2 cell acquisition of dietary iron(III) invokes a nanoparticulate endocytic pathway
... for Caco-2 cell differentiation and the integrity of the cell monolayer was assured prior to the experiments (see Figure ...± 2% microparticulate iron at 30 min) prevented iron utilization (Figure ...
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Platelet aggregation induced by Caco 2 cells: regulation by matrix metalloproteinase 2 and adenosine diphosphate
... that Caco-2 cells increased the number of copies of GPIIb/IIIa (both nonacti- vated and activated receptors), GPIb, and P-selectin, as mea- sured by flow ...
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DOCOSAHEXAENOIC ACID INDUCES APOPTOSIS IN COLON CANCER CELL LINE, Caco-2
... NaCl, 2-propanol, phosphate-buffered saline (PBS), ethidium bromide, agarose gel, Peroxidase, trypsin, Hematoxylin and eosin (Hx & E) stain, primary monoclonal antibody against Bcl-2 and biotinylated ...
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The expression of mature human calcitonin in Caco-2 cells
... in Caco-2 ceUs one could use the vector mentioned previously (Leff et aL, ...gene, Caco-2 cells transfected with this construct and mRNA production studied by Northern ...
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Curcumin inhibits cholesterol uptake in Caco-2 cells by down-regulation of NPC1L1 expression
... expression of LDL receptor [16,17]. Since intestinal absorption of cholesterol also affects the levels of choles- terol in the blood, whether uptake of cholesterol in the enterocytes can be affected by curcumin is ...
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