Caco-2 cell monolayer
In vitro digestion and lactase treatment influence uptake of quercetin and quercetin glucoside by the Caco-2 cell monolayer
... of Caco-2 cells was measured using a method adapted from Dahlqvist ...the cell homogenates and the final colored products were measured at 420 nm using a spectrophotometer ...the Caco-2 ...
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The octanol/water distribution coefficients of ardipusilloside-I and its metabolites, and their permeation characteristics across Caco-2 cell monolayer
... 14]. Caco-2 cell monolayer has been widely accepted as a standard in vitro model for prediction of drug absorption across human intestine and for mecha- nistic studies of intestinal drug ...
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Enhanced uptake and transport of (+)-catechin and (-)-epigallocatechin gallate in niosomal formulation by human intestinal Caco-2 cells
... intestinal Caco-2 cell monolayer in both the absence and presence of niosomal carrier in variable ...of Caco-2 cells was ...the monolayer of the human Caco-2 ...
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Intestinal absorbability of three Radix Puerariae isoflavones including daidzein, daidzin and puerarin
... the Caco-2 cell monolayer with its concentration in receiver chamber not being detectable until two ...the monolayer with the passive diffusion ...
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Mucosal and blood brain barrier transport kinetics of the plant N alkylamide spilanthol using in vitro and in vivo models
... than 2-fold, indicating that no active transport function can be readily ...a Caco-2 cell monolayer experiment and oral absorption in humans [31, 34, ...
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Original Article Salvianolic acid B ameliorates hydrogen peroxide-induced barrier disruption in monolayer Caco-2 cells
... of Caco-2 cell monolayers exposed to hydrogen peroxide (H 2 O 2 ...). Cell metabolic activity and barrier integrity were assessed by measuring lactate dehydrogenase (LDH) ...
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Multiple nanoemulsion system for an oral combinational delivery of oxaliplatin and 5-fluorouracil: preparation and in vivo evaluation
... and Caco-2 cell ...adenocarcinoma cell (CT26)- bearing mouse model were investigated after oral administration of nanoemulsion containing OXA/DCK and ...a Caco-2 cell ...
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Uptake and transport of a novel anticancer drug-delivery system: lactosyl-norcantharidin-associated N-trimethyl chitosan nanoparticles across intestinal Caco-2 cell monolayers
... (Caco-2) cell monolayer model was used to study the transport mechanism of Lac-NCTD, and the effects of factors such as time, temperature, pH level, drug concentration, enhancers, and ...
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Candida albicans Sun41p, a Putative Glycosidase, Is Involved in Morphogenesis, Cell Wall Biogenesis, and Biofilm Formation
... the cell wall-disturbing agent Congo red, whereas no difference was observed in the presence of calcofluor ...a Caco-2 cell monolayer, and were unable to form hyphae on solid medium ...
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Enhanced oral bioavailability of docetaxel by lecithin nanoparticles: preparation, in vitro, and in vivo evaluation
... method. Caco-2 cell monolayer was used for the in vitro permeation study of ...the Caco-2 cell monolayer from the DTX-LNs was ...
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Short communication: A study of Lactobacillus isolates' adherence to and influence on membrane integrity of human Caco-2 cells
... the Caco-2 cell monolayer, which reflects the intestinal barrier function of the epithelium cells, can be determined and measured in vitro in terms of trans-epithelial electrical resistance ...
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Enhanced oral absorption of pemetrexed by ion-pairing complex formation with deoxycholic acid derivative and multiple nanoemulsion formulations: preparation, characterization, and in vivo oral bioavailability and anticancer effect
... epithelial cell uptake of PMX were synergistically improved after HP-beta-CD/PMX/DCK/P188 was incorporated into a nanoemulsion with a size of ...a Caco-2 cell monolayer was ...carcinoma ...
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A Novel Role of OS-9 in the Maintenance of Intestinal Barrier Function from Hypoxia-induced Injury via p38-dependent Pathway
... OS-9 is a lectin required for efficient ubquitination of glycosylated substrates of endoplasmic reticu- lum-associated degradation (ERAD). OS-9 has previously been implicated in ER-to-Golgi transport and transcription ...
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Pseudomonas fluorescens alters epithelial permeability and translocates across Caco-2/TC7 intestinal cells
... differentiated Caco-2/TC7 cells cultivated on insert, no significant cytotoxicity was observed, even after 24 h of infection with these two ...differentiated Caco-2/TC7 cells explaining the ...
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Permeation of roxarsone and its metabolites increases caco 2 cell proliferation
... a monolayer of epithe- lial cells, Transwell studies using Caco-2 cells were per- ...which Caco-2 cells were treated for 24 hours with 10 μM (15 nmol) of one of the benzenearsonates ...
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APOPTOTIC EFFECT OF GOLD NANOPARTICLES ON HUMAN COLON CANCER CELL LINES CACO 2 BUT NOT ON THE NORMAL EPITHELIAL CELL LINES WISH
... The monolayer cell cultures of Caco-2 and WISH cell lines were separately trypsinized ...final cell density of 10*104 ...and cell monolayers were fixed with 10% ...
22
DOCOSAHEXAENOIC ACID INDUCES APOPTOSIS IN COLON CANCER CELL LINE, Caco-2
... NaCl, 2-propanol, phosphate-buffered saline (PBS), ethidium bromide, agarose gel, Peroxidase, trypsin, Hematoxylin and eosin (Hx & E) stain, primary monoclonal antibody against Bcl-2 and biotinylated ...
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Lipid phosphatase SHIP2 functions as oncogene in colorectal cancer by regulating PKB activation
... cancer-related death. This is mainly due to the fact that around 20% of colorectal cancer patients are diagnosed with metastatic disease, drastically dropping their 5-year survival from 90% in non-metastatic patients, to ...
16
Quantitative Estimation of P-glycorptein-Mediated Drug Transport by Mechanistically Modeled Intrinsic Clearance
... Drug-drug interactions are a real concern because of their potential to push serum digoxin concentrations outside of the narrow therapeutic window. Several clinically significant DDIs involving digoxin have been ...
147
Vol 71, No 1 (2019)
... cancer cell lines are ...derived cell lines (Caco-2, MCF-7, SK-BR-3, U-87 and U-251) express ...that Caco-2 cells originating from colorectal adenocarcinoma express prothrombin, ...
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