cell-free mRNA translation
Cell-free translation of influenza virus mRNA.
... the two major translation products obtained in vitro in the wheat germ system programmed with infected cell mRNA to the corresponding proteins M and NP derived from purified virus.. Thes[r] ...
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Poly(rC) Binding Proteins and the 5′ Cloverleaf of Uncapped Poliovirus mRNA Function during De Novo Assembly of Polysomes
... PV mRNA for limiting factors with endogenous host mRNAs was minimal in the cell-free reaction mixtures and that endogenous host mRNAs have little impact on the ability of PV mRNA to form ...
12
Transcriptional and translational mapping of a 6.6-kilobase-pair DNA fragment containing the junction of the terminal repetition and unique sequence at the left end of the vaccinia virus genome.
... Hybridization selection and cell-free translation of mRNA's encoded within the inverted terminal repetition of the vaccinia virus genome.. Extension of the transcriptional and translatio[r] ...
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Visualizing mRNA Translation in situ in Single Cells
... from cell fixation to image collection and ...and mRNA, FLARIM requires fewer steps and is enzyme-free, making it cheaper and easier to modify for different sample ...mRNA translation. ...
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Ligation free ribosome profiling of cell type specific translation in the brain
... purify mRNA–ribosome complexes that originate from Camk2a-expressing neurons by immunoprecipitation (IP) of the HA-tag ...other cell types in the brain, including inhibitory neu- rons, were depleted by IP ...
15
The relationship between genotype and phenotype in cell-free transcription-translation reactions.
... In order to properly test if this approach could remove the influence of mRNA secondary structures from gene expression in our system we designed both a positive and a negative control. The positive control would ...
92
DNA-dependent RNA polymerase subunits encoded within the vaccinia virus genome.
... Our basic approach to mapping the RNA polymerase subunits was to immunoprecipitate reticulocyte cell-free translation products of mRNA that were selected by hybridization to cloned vacci[r] ...
7
Effect of RNase III on efficiency of translation of bacteriophage T7 lysozyme mRNA.
... Because differences other than RNase III activity might influence the efficiency of messenger translation in crude cell-free extracts, we attempted to stimulate translation of presumably[r] ...
12
Translation of mRNA from Simian Virus 40-Infected Cells into Simian Virus 40 Capsid Protein by Cell-Free Extracts
... Nonetheless, analysis of the product of a reticulocyte incubation containing mRNA from SV40-infected cells yields a labeled protein species which comigrates with authentic SV40 capsid, a[r] ...
6
Normal translation of human adenovirus mRNA in cell-free lysates prepared from abortively as well as productively infected monkey cells.
... from abortively infected Ad2 monkey cells and To determine whether the poor utilization of from productively infected monkey cells where the residual fiber RNA made in abortively in- the[r] ...
5
Cell-free translation of murine coronavirus RNA.
... The six murine hepatitis virus-specific subgenomic RNAs were partially purified by agarose gel electrophoresis and translated in an mRNA-dependent rabbit reticulocyte lysate, and the cel[r] ...
9
Peptide mapping characterization of viral proteins generated in a cell-free coupled system for the transcription and translation of influenza virus mRNA.
... In the coupled cell-free system for transcription and translation of the influenza mRNA's, using the fowl plague virus, we could show the synthesis of virion proteins M and NP and two pe[r] ...
5
Translation and processing of mouse hepatitis virus virion RNA in a cell-free system.
... Either leupeptin or zinc chloride was added to the lysate before the addition of MHV-A59 mRNA, and translation reactions were incubated for 90 min in the presence of [35S]methionine: Lan[r] ...
7
Mapping of the vaccinia virus DNA polymerase gene by marker rescue and cell-free translation of selected RNA.
... The location of the DNA polymerase gene, about 57 kilobases from the left end of the genome, was confirmed by cell-free translation of mRNA selected by hybridization to plasmids containi[r] ...
6
Transcriptional mapping of the DNA polymerase gene of vaccinia virus.
... Mapping of the vaccinia virus thymidine kinase gene by marker rescue and by cell-free translation of selected mRNA.. Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest LIT[r] ...
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Translation of Virus mRNA: Synthesis of Bacteriophage Qβ Proteins in a Cell-Free Extract from Wheat Embryo
... Translation of Virus mRNA: Synthesis of Bacteriophage Q,B Proteins in a Cell-Free Extract from Wheat Embryo Received for publication 24 August 1973 The RNA from bacteriophage QB can be t[r] ...
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Study of messenger RNA inactivation and protein degradation in an Escherichia coli cell-free expression system
... of mRNA and the deg- radation rate of proteins have been studied in a transcrip- tion-translation cell-free ...a cell-free expression system driven by the endogenous ...
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Highly Diverged Homologs of Saccharomyces cerevisiae Mitochondrial mRNA-Specific Translational Activators Have Orthologous Functions in Other Budding Yeasts
... the mRNA-spe- lational activation system uncovered in ...among mRNA 5⬘-UTLs also appear to have evolved ...coded mRNA 5⬘-UTL drially coded protein sequences, which are known to targets have diverged ...
14
5AP Review Molecular Genetics and Biotechnology.pdf
... The arrival of a large ribosomal subunit completes the initiation complex. Proteins called initiation factors (not shown) are required to bring all the translation components together. GTP provides the energy for ...
11
In Vitro Translation of Cardiovirus Ribonucleic Acid by Mammalian Cell-free Extracts
... To compare the product of the in vitro incubaME RNAtion with the proteins made in virus-infected cells, directed incorporation Supernatant fraction L cell monolayers were infected with M[r] ...
10