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Cell-free translation system

Capsid intermediates assembled in a foot-and-mouth disease virus genome RNA-programmed cell-free translation system and in infected cells.

Capsid intermediates assembled in a foot-and-mouth disease virus genome RNA-programmed cell-free translation system and in infected cells.

... DISCUSSION Our results indicate that i various capsid intermediate structures are present in FMDV-infected cells, ii 14S particles in infected cells are relatively short lived, iii virus[r] ...

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Toward an in vitro system for picornavirus assembly: purification of mengovirus 14S capsid precursor particles.

Toward an in vitro system for picornavirus assembly: purification of mengovirus 14S capsid precursor particles.

... 275 -- + Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Mengovirus 14S subviral protein particles generated in infected L cells and in a cell-free translation system p[r] ...

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The membrane-associated and secreted forms of the respiratory syncytial virus attachment glycoprotein G are synthesized from alternative initiation codons.

The membrane-associated and secreted forms of the respiratory syncytial virus attachment glycoprotein G are synthesized from alternative initiation codons.

... Analysis of the proteins synthesized from these cDNAs, either by translation of transcripts in a cell-free system or in cells infected with recombinant vaccinia viruses containing either[r] ...

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Stimulation of poliovirus RNA synthesis and virus maturation in a HeLa cell free in vitro translation RNA replication system by viral protein 3CDpro

Stimulation of poliovirus RNA synthesis and virus maturation in a HeLa cell free in vitro translation RNA replication system by viral protein 3CDpro

... made viral RNAs. The involvement of VPg in encapsida- tion has been previously proposed by Reuer et al. [15] who observed that some lethal VPg mutations still permit near normal minus and plus strand RNA synthesis in ...

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Effect of UV irradiation on the expression of vaccinia virus gene products synthesized in a cell-free system coupling transcription and translation.

Effect of UV irradiation on the expression of vaccinia virus gene products synthesized in a cell-free system coupling transcription and translation.

... In considering the coupling of vacinia mM GTP, 0.5 mM CTP, 200 mCi of r3HJUTP per at a final concentration of 0.1 mM; and 1 OD20 virus-directed RNA synthesis to translation, we 1tmol of [r] ...

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The relationship between genotype and phenotype in cell-free transcription-translation reactions.

The relationship between genotype and phenotype in cell-free transcription-translation reactions.

... In order to properly test if this approach could remove the influence of mRNA secondary structures from gene expression in our system we designed both a positive and a negative control. The positive control would ...

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Translation and processing of mouse hepatitis virus virion RNA in a cell-free system.

Translation and processing of mouse hepatitis virus virion RNA in a cell-free system.

... Either leupeptin or zinc chloride was added to the lysate before the addition of MHV-A59 mRNA, and translation reactions were incubated for 90 min in the presence of [35S]methionine: Lan[r] ...

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Translation of murine leukemia virus RNA in cell-free systems from animal cells.

Translation of murine leukemia virus RNA in cell-free systems from animal cells.

... Fingerprint analysis of tryptic digests of polypeptide products formed in the cell-free system in B Polypeptides 60 and 70K synthesized in the L-cell-free system to 140,000-molecular-wei[r] ...

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Stimulation of Poliovirus Synthesis in a HeLa Cell-Free In Vitro Translation-RNA Replication System by Viral Protein 3CDpro

Stimulation of Poliovirus Synthesis in a HeLa Cell-Free In Vitro Translation-RNA Replication System by Viral Protein 3CDpro

... be observed both in vivo and in vitro in small amounts, partic- ularly during the early hours of translation of poliovirus RNA (32). In other picornaviruses, such as encephalomyocarditis virus (EMCV) or hepatitis ...

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Proteolytic processing of foot-and-mouth disease virus polyproteins expressed in a cell-free system from clone-derived transcripts.

Proteolytic processing of foot-and-mouth disease virus polyproteins expressed in a cell-free system from clone-derived transcripts.

... Cell-free translation of foot-and-mouth disease virus FMDV RNA in a rabbit reticulocyte system produces functional proteins, including viral protease 3C, which plays a major role in proc[r] ...

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Regulation of Brome Mosaic Virus Gene Expression by Restriction of Initiation of Protein Synthesis

Regulation of Brome Mosaic Virus Gene Expression by Restriction of Initiation of Protein Synthesis

... 330 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The translation of total and individual brome mosaic virus BMV RNAs was examined in a wheat germ cell-free system in[r] ...

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Stögbauer, Tobias Roland
  

(2012):


	Experiment and quantitative modeling of cell-free gene expression dynamics.


Dissertation, LMU München: Fakultät für Physik

Stögbauer, Tobias Roland (2012): Experiment and quantitative modeling of cell-free gene expression dynamics. Dissertation, LMU München: Fakultät für Physik

... particular cell-free system which is generally not ...a cell-free system through a combination of a set of calibration experiments and a mathematical model is ...of ...

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Prototyping Diverse Synthetic Biological Circuits in a Cell Free Transcription Translation System

Prototyping Diverse Synthetic Biological Circuits in a Cell Free Transcription Translation System

... the cell-free reaction, both plasmids and PCR products underwent an additional PCR purification step using a QiaQuick column (Qiagen), which removed excess ...

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Cell-free translation of influenza virus mRNA.

Cell-free translation of influenza virus mRNA.

... the two major translation products obtained in vitro in the wheat germ system programmed with infected cell mRNA to the corresponding proteins M and NP derived from purified virus.. Thes[r] ...

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Translation of Pseudomonas aeruginosa Bacteriophage PP7 RNA by a Cell-Free Amino Acid Incorporating System from Escherichia coli

Translation of Pseudomonas aeruginosa Bacteriophage PP7 RNA by a Cell-Free Amino Acid Incorporating System from Escherichia coli

... The only identifiable product of the PP7 RNA-directed reaction has an electrophoretic mobility identical to that of PP7 coat protein isolated from mature phage particles, indicating that[r] ...

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Template Recognition Mechanisms by Replicase Proteins Differ between Bipartite Positive-Strand Genomic RNAs of a Plant Virus

Template Recognition Mechanisms by Replicase Proteins Differ between Bipartite Positive-Strand Genomic RNAs of a Plant Virus

... a translation inhib- ...a translation/replication cell-free system prepared from evacu- olated BY-2 tobacco protoplasts (BYL) (26), which has been used for the study of RCNMV (1, 18, ...

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Peptide mapping characterization of viral proteins generated in a cell-free coupled system for the transcription and translation of influenza virus mRNA.

Peptide mapping characterization of viral proteins generated in a cell-free coupled system for the transcription and translation of influenza virus mRNA.

... In the coupled cell-free system for transcription and translation of the influenza mRNA's, using the fowl plague virus, we could show the synthesis of virion proteins M and NP and two pe[r] ...

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Integrating stakeholder perspectives into the translation of cell-free fetal DNA testing for aneuploidy

Integrating stakeholder perspectives into the translation of cell-free fetal DNA testing for aneuploidy

... into translation include pregnant women and their families; healthcare providers; scientists, their institutions or companies, and the funding agencies that support them; regulatory and judicial bodies; ...

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Students’ Common Mistakes in Technical Texts When Using Computer Technologies

Students’ Common Mistakes in Technical Texts When Using Computer Technologies

... machine translation of technical texts to make sure the process of exchange of scientific and technical information is sufficient still does not correspond to specified ...machine translation systems (HMT) ...

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Use of humanised rat basophilic leukaemia cell line RS ATL8 for the assessment of allergenicity of Schistosoma mansoni proteins

Use of humanised rat basophilic leukaemia cell line RS ATL8 for the assessment of allergenicity of Schistosoma mansoni proteins

... Serum samples which had undergone anti-viral treatment with a mixture of detergents had to be diluted at least 100-fold, as higher concentrations of detergents led to complete destruction of cells as assessed by ...

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