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cell sorting

Applications of cell sorting in biotechnology

Applications of cell sorting in biotechnology

... a cell sorting frame, as binding of the fluorescent probe to the cell is an intrinsic feature of the desired ...the cell that produced an enzyme catalyzing this ...expressing cell via ...

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The use of fluorescence activated cell sorting in studying plant development and environmental responses

The use of fluorescence activated cell sorting in studying plant development and environmental responses

... Fluorescence-Activated Cell Sorting (FACS) ...conferring cell- or tissue-specific expression are chosen for an experi- ment; for instance, Medicago truncatula roots expressing GFP expressed in the ...

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Differential adhesion and actomyosin cable collaborate to drive Echinoid mediated cell sorting

Differential adhesion and actomyosin cable collaborate to drive Echinoid mediated cell sorting

... Ed-mediated cell sorting is similar to the process of dorsal ...Ed-mediated cell sorting involves the polarized assembly of actomyosin cable only in the wild-type interface ...of cell ...

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Cell specific analysis of Arabidopsis leaves using fluorescence activated cell sorting

Cell specific analysis of Arabidopsis leaves using fluorescence activated cell sorting

... Grønlund, Jesper T., Eyres, Alison, Kumar, S., Buchanan-Wollaston, Vicky and Gifford, M. L.. (2012) Cell Specific Analysis of Arabidopsis Leaves Using Fluorescence Activated Cell Sorting. Journal of ...

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The use of fluorescence activated cell sorting in studying plant development and environmental responses

The use of fluorescence activated cell sorting in studying plant development and environmental responses

... Fluorescence-Activated Cell Sorting (FACS) ...conferring cell- or tissue-specific expression are chosen for an experi- ment; for instance, Medicago truncatula roots expressing GFP expressed in the ...

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High-Intensity Ultrasound in Adherent Cell Sorting and Tissue Ablation.

High-Intensity Ultrasound in Adherent Cell Sorting and Tissue Ablation.

... epithelial cell population with several stem/progenitor cell qualities have been identified ...these cellcell interactions, cytolysis, and secretory activity is not well ...NK ...

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Biotechnologies for Cancer Diagnostics: Cell Sorting, Protein Analysis and Imaging of Cellular Metabolism

Biotechnologies for Cancer Diagnostics: Cell Sorting, Protein Analysis and Imaging of Cellular Metabolism

... Levels of proteins secreted from single-cells can exhibit a variability that reflects the stochastic nature of biology 17 and, in fact, represents the biological fluctuations. The SCBC experimental error must be compared ...

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Cell fusion in tumor progression: the isolation of cell fusion products by physical methods

Cell fusion in tumor progression: the isolation of cell fusion products by physical methods

... fluorescent cell sorting [24,25] have been used to isolate cell fusion pro- ducts, although both procedures have several ...drawbacks. Cell separation by physical methods provides a possible ...

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An engineered autotransporter-based surface expression vector enables efficient display of Affibody molecules on OmpT-negative E. coli as well as protease-mediated secretion in OmpT-positive strains

An engineered autotransporter-based surface expression vector enables efficient display of Affibody molecules on OmpT-negative E. coli as well as protease-mediated secretion in OmpT-positive strains

... Multiple sorting rounds are usually required for enrich- ment of high-affinity binders from large libraries and the possibility to amplify isolated clones by cell growth in between the cycles greatly ...

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Blood cells sorting using cotton threads

Blood cells sorting using cotton threads

... in cell biology and biological assays since they made possible the controlling of environment properties at the cell ...including cell sorting. In last decade new concepts were proposed for ...

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Combination of BCL11A siRNA with vincristine increases the apoptosis of SUDHL6 cells

Combination of BCL11A siRNA with vincristine increases the apoptosis of SUDHL6 cells

... Transfected SUDHL6 cells were harvested after treatment. Morphology was determined with Hoechst 33258 following incubation for 72 h. Cells were washed with PBS three times and then stained with 10 μl Hoechst33258 nuclear ...

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Antitumor Efficacy of Human Monocyte-Derived Dendritic Cells: Comparing Effects of two Monocyte Isolation Methods

Antitumor Efficacy of Human Monocyte-Derived Dendritic Cells: Comparing Effects of two Monocyte Isolation Methods

... where cell purity is not a major concern [9, 10]. To obtain high cell purity, the immunomagnetic separation is preferred and two of the widely used immunomagnetic separation technologies are magnetic ...

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Rapid production of antigen-specific monoclonal antibodies from a variety of animals

Rapid production of antigen-specific monoclonal antibodies from a variety of animals

... Figure 4 Isolation of rabbit antigen-specific plasma/plasmablast cells (ASPCs) using fluorescence-activated cell sorting (FACS). (A) A representative FACS graph of the lymphocytes of green fluorescent ...

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Monocyte galactose/N-acetylgalactosamine-specific C-type lectin receptor stimulant immunotherapy of an experimental glioma. Part II: combination with external radiation improves survival

Monocyte galactose/N-acetylgalactosamine-specific C-type lectin receptor stimulant immunotherapy of an experimental glioma. Part II: combination with external radiation improves survival

... Methods: C57BL/6 mice underwent stereotactic intracranial implantation of GL261 glioma cells. Animals were grouped based on randomized tumor size by magnetic resonance imaging on day seven. One group that received ...

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Clonally expanded human airway smooth muscle cells exhibit morphological and functional heterogeneity

Clonally expanded human airway smooth muscle cells exhibit morphological and functional heterogeneity

... single cell sorting in 96 well culture plates using Coulter Epics Altra Hypersort fluorescence-activated cell sorter ...the cell sort gating criteria ensured that only cells with similar ...

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VHDL Design of a Scalable VLSI Sorting Device Based on Pipelined Computation

VHDL Design of a Scalable VLSI Sorting Device Based on Pipelined Computation

... a sorting algorithm, aiming at defining an elementary sorting unit as a building block of VLSI devices which require a huge number of sorting ...A sorting VLSI device, in fact, can be built as ...

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Arrays

Arrays

... Parallel sorting of large arrays on multiprocessor systems is faster than sequential array sorting.[r] ...

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Educational Alternatives, Volume 16, 2018

Educational Alternatives, Volume 16, 2018

... These multimedia applications provide a comprehensive view on the issue of processing postal mails in the company PB Partner a.s. Žilina (Aupark) and HSS Žilina 022, which is the main sorting center located in ...

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Thesis

Thesis

... mitotic cell lineages: absorptive enterocytes, goblet cells, enteroendocrine cells, and Paneth cells (Figure ...these cell types continue to migrate upward into the large finger-like villi, where they live ...

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Suberoylanilide hydroxamic acid induces apoptosis and sub-G1 arrest of 320 HSR colon cancer cells

Suberoylanilide hydroxamic acid induces apoptosis and sub-G1 arrest of 320 HSR colon cancer cells

... For MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenylte- trazolium bromide) assays, 320 HSR cells were cultured in 96-well culture plates at a density of 7000 cells/well with 200 μ L culture medium. Suberoylanilide ...

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