Cell surface display
Development of a novel rDNA based plasmid for enhanced cell surface display on Yarrowia lipolytica
... for cell surface display on ...enhanced cell surface display of heterologous proteins on ...to display heterologous pro- teins on Y. lipolytica cell surface ...
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Engineering the cell surface display of cohesins for assembly of cellulosome-inspired enzyme complexes on Lactococcus lactis
... the cell surface display of small cellulosome scaffold proteins in Lactococcus lactis, a first and necessary step for the eventual engineering of extracellular protein complexes in this, and other ...
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Efficient yeast cell-surface display of exo- and endo-cellulase using the SED1 anchoring region and its original promoter
... Multiple enzymes, including endo-type cellulases, are necessary for the hydrolysis of cellulose [5]. EG, which cleaves glycosidic linkages randomly along cellulose chains, is also one of the enzymes required for the ...
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Combined cell-surface display- and secretion-based strategies for production of cellulosic ethanol with Saccharomyces cerevisiae
... the cell col- lection step at the beginning of each ...by cell-surface dis- play strategy is more applicable to CRBF compared with the secretion ...the cell-surface display ...
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RGD Peptide Cell-Surface Display Enhances the Targeting and Therapeutic Efficacy of Attenuated Salmonella-mediated Cancer Therapy
... Microbial cell-surface display systems allow peptides and proteins to be displayed on the surface of microbial cells by fusing them with an anchoring motif; these motifs are usually ...
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Improved cell surface display of Salmonella enterica serovar Enteritidis antigens in Escherichia coli
... All cultivations were performed in a batch format ini- tialized by shake flask growth in 1 L bottles containing 100 ml of minimal salts medium supplemented with 10 g l −1 glucose. These were inoculated from a frozen ...
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Immune Evasion Proteins of Murine Cytomegalovirus Preferentially Affect Cell Surface Display of Recently Generated Peptide Presentation Complexes
... downmodulate cell surface MHC-I (34, 62, 89) and inhibit the presentation of antigenic peptides to CD8 T cells (34, ...of cell surface class I molecules and does not inhibit peptide ...
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Constitutive expression and cell-surface display of a bacterial β-mannanase in Lactobacillus plantarum
... the cell wall and thus not sufficiently exposed for recognition by the antibody used for Myc ...of surface- displayed ManB was detected for the strain carrying the plasmid pPgm_Lp1261ManB and the highest ...
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Cell Surface Display of Red Grouper Nervous Necrosis Virus Capsid Protein on Pichia pastoris
... the cell surface, sur- face-immobilized proteins were covalently linked to glucan in the cell wall, ren- dering them more stable over a period of ...the cell wall for implication as an oral ...
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Cell Surface Display of Human Immunodeficiency Virus Type 1 gp120 on Escherichia coli by Using Ice Nucleation Protein
... INP-based cell sur- face display has many advantages compared to other gram- negative bacterial surface expression ...the cell surface can be detected indirectly by ice nucleation ...
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Probing the applicability of autotransporter based surface display with the EstA autotransporter of Pseudomonas stutzeri A15
... after surface display and the absence of the right-handed parallel β-helix structure ...to display a β-lactamase [17] and Pseudomonas and Burkholderia lipases at the cell surface of ...
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Peptide-mediated microalgae harvesting method for efficient biofuel production
... to cell floc- ...steady cell growth and oil accumulation as much as the wild-type system (Additional file 1: ...the cell-surface display system established in this study does not have a ...
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A Unique and Conserved Neutralization Epitope in H5N1 Influenza Viruses Identified by an Antibody against the A/Goose/Guangdong/1/96 Hemagglutinin
... yeast cell surface ...yeast cell surface dis- ...yeast cell surface display and analyzed by fluorescence-acti- vated cell sorting (FACS) of flow cytometry ...
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Heterologous protein display on the cell surface of lactic acid bacteria mediated by the s-layer protein
... The display of heterologous proteins on the cell surface of lactic acid bacteria (LAB) is an exciting and emerging research area that holds great promise for a variety of biotechnological ...
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Antigen-43-mediated surface display revealed in Escherichia coli by different fusion sites and proteins
... the surface display of the passen- ger domain (Jose 2006). Cell surface display based on ATs has been reported to be one of the most attractive systems for displaying large and complex ...
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Dna Double Strand Breaks Suppress Expression Of The Rag Recombinase: Mechanisms And Consequences
... lineage cells and allows these cells to survive in culture and protects cells from apoptosis upon exposure to DNA damage (Strasser et al., 1991a, 1991b). To determine the effect of non-RAG mediated DSBs on pre-B cells, ...
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Expression of a 28-Kilodalton Glutathione S-Transferase Antigen of Schistosoma mansoni on the Surface of Filamentous Phages and Evaluation of Its Vaccine Potential
... the surface of phage. Display of Sm28GST on the surface of phage in pdGST was evaluated by an enzyme-linked immunosorbent assay (ELISA) as described previously ...
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Epithelial-to-mesenchymal transition leads to loss of EpCAM and different physical properties in circulating tumor cells from metastatic breast cancer
... in cell- cell adhesion was observed, which was associated with a marked decrease in E-cadherin expression and an increase in N-cadherin in sphere cells as measured by Western blotting and immunofluorescence ...
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Surface display of Salmonella epitopes in Escherichia coli and Staphylococcus carnosus
... and it is suggested that the cleavage is partly carried out by the same proteases for both the proteins. The degra- dation takes place either in the periplasm or in the OM since an uncleaved N-terminal signal must have ...
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Efficient yeast surface-display of novel complex synthetic cellulosomes
... assembled cellulases was clearly observed in strains where ScafAGA3 was co-expressed with Te-aCBH1, Ct- aCelA and Sf-aBGL1 (Fig. 4). The results of FACS anal- ysis further confirmed the self-assembly of the three ...
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