CHO-K1 cell line
Establishment a CHO Cell Line Expressing Human CD52 Molecule
... To determine the appropriate concentration of Zeocin, MTT assay was done (9). Approximately 3×10 5 -5×10 5 of passage 3-5 CHO-K1 cell line were cultured in 96-well flat bottom plates with 10% ...
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Transfecting CHO-K1 Cells: Comparison of CaPO4, Electroporation and Lipoplex Method with In-house Prepared Polyplex
... assay. CHO-K1 cell line was procured from National Centre For Cell Science (NCCS), Pune, India and maintained in DMEM medium containing 10% FBS at 37° in 5% CO 2 .... ...
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Iron regulated surface determinant B (IsdB) promotes Staphylococcus aureus adherence to and internalization by non phagocytic human cells
... platelet integrin αIIbβ3 we used a CHO-K1 cell line stably expressing human wild-type 171.. αIIbβ3 and the N305T mutant locked in the high affinity conformation by an N-172.[r] ...
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Mutations That Affect the Tropism of DA and GDVII Strains of Theiler's Virus In Vitro Influence Sialic Acid Binding and Pathogenicity
... new cell line by varying the flexible loops located at the surface of the capsid without affecting the structural integ- rity of the ...and CHO-K1 cells probably increased the affinity of the ...
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Structural Features of Nectin-2 (HveB) Required for Herpes Simplex Virus Entry
... confirms cell surface expression and retention of functional activity by the chimeric ...and cell surface ...determine cell surface expression of the various nectin-2 molecules, CHO-K1 ...
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Disclosing the CXCR4 Expression in Lymphoproliferative Diseases by Targeted Molecular Imaging
... lymphocyte cell line Jurkat was purchased from American Type Culture Collection (ATCC) and maintained in RPMI-1640 medium sup- plemented with 10% FCS, 2 mM L-glutamine, and 100 units/mL of ...
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PG13 Packaging Cells Produce Recombinant Retroviruses Carrying a Diphtheria Toxin Mutant Which Kills Cancer Cells
... selected CHO-K1 cell lines that contain one or both EF-2 alleles that were mutated, making these cells partially or completely resistant to DT (6, 7, 11, 12, 19, ...packaging cell lines ...
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Enhanced optical manipulation of cells using anti reflection coated microparticles
... adherent CHO-K1 and NMuMG cells as well as the non-adherent HL60 cells, were considerably lower than those obtained for the either of the native particle ...the cell lines following the ...
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Equine Herpesvirus 1 Entry via Endocytosis Is Facilitated by αV Integrins and an RSD Motif in Glycoprotein D
... In line with this observation, it is interesting that EHV-1 gB and gH, two glycoproteins important for herpesviral entry, each contain integrin binding ...mediate cell entry and therefore infectivity of ...
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Ovine Herpesvirus 2 Glycoproteins B, H, and L Are Sufficient for, and Viral Glycoprotein Ov8 Can Enhance, Cell-Cell Membrane Fusion
... fibroblast line (A113) was used as the target, coculture with effector cells expressing gB and gH/gL resulted in luciferase expression significantly higher than that of the control (P ⬍ ...transfected ...
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Vaccinia Virus A25 and A26 Proteins Are Fusion Suppressors for Mature Virions and Determine Strain-Specific Virus Entry Pathways into HeLa, CHO-K1, and L Cells
... cells. Cell type-specific and virus strain-specific differences have been ob- served; however, the molecular basis driving such differences is not ...HeLa, CHO-K1, and L ...
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Introduction of human genomic sequences renders CHO-K1 cells susceptible to infection by amphotropic retroviruses.
... The 1-gal titer of this ecotropic vector was similar to that of the PA317/LBgSN-conditioned medium when tested on NIH Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest To [r] ...
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Heparan Sulfate Facilitates Rift Valley Fever Virus Entry into the Cell
... R ift Valley fever virus (RVFV) belongs to the Phlebovirus genus of the Bunyaviridae family. Its negative-stranded tripartite RNA genome is encapsidated by nucleocapsid protein and is sur- rounded by a lipid-containing ...
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Engineered CHO cell line with coxsackie and adenovirus gene enhance the susceptibility of adenovirus infection
... Wang, B., Chen, G., Zhou, J., Wu, P., Luo, D., Huang, X., Zhu, T., Han, Z., Xu, G., Wang, S., Lu, Y. and Ma, D. (2007). Deletion of the intracellular domain of coxsackie and adenovirus receptor (CAR) enhances the ...
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Repair of DNA interstrand cross-links induced by anticancer agents in mammalian cells
... Although cisplatin ICLs are processed without a DSB intermediate, the HR pathway is still required in the processing of cisplatin induced DNA damage, as the XRCC2 and XRCC3 mutants are extremely sensitive to cisplatin. ...
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Coexpression of ATP binding cassette proteins ABCG5 and ABCG8 permits their transport to the apical surface
... Mutations in either ATP-binding cassette (ABC) G5 or ABCG8 cause sitosterolemia, an autosomal recessive disorder of sterol trafficking. To determine the site of action of ABCG5 and ABCG8, we expressed recombinant, ...
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A mutant CHO-K1 strain with resistance to Pseudomonas exotoxin A and alphaviruses fails to cleave Sindbis virus glycoprotein PE2.
... DISCUSSION In this article we report that RPE.40, a mutant strain of CHO-Kl cells selected for resistance to Pseudomonas exotoxin A and cross-resistant to alphaviruses 21, fails to cleav[r] ...
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Discovery of novel adenosine receptor agonists that exhibit subtype selectivity
... activity of 37 (CVT-3619) was previously demonstrated in rat adipocytes where it reduced cAMP content and consequently lipolysis 14 although it is entirely possible that the observed response in these cell lines ...
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The Ig-Like V-Type Domain of Paired Ig-Like Type 2 Receptor Alpha Is Critical for Herpes Simplex Virus Type 1-Mediated Membrane Fusion
... and cell fusion activity for the amino acid substitution and deletion ...CELISA, CHO-K1 cells were transfected in 96-well plates with the various amino acid substitution and deletion ...For ...
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Analysis of the unique hamster cell tropism of ecotropic murine leukemia virus PVC-211.
... enters CHO-K1 cells is not known. CHO-K1 cells express at least three classes of retroviral receptors: a modified ecoR, a GaLV receptor (PiT1), and an amphotropic MuLV receptor (PiT2) (30, 31, ...
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