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clinical samples

Whole genome enrichment and sequencing of Chlamydia trachomatisdirectly from clinical samples

Whole genome enrichment and sequencing of Chlamydia trachomatisdirectly from clinical samples

... Clinical samples often contain low numbers of patho- gens and to obtain sufficient material for WGS of ...positive samples are particularly ...complex clinical samples but required in ...

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Optimization and validation of sample preparation for metagenomic sequencing of viruses in clinical samples

Optimization and validation of sample preparation for metagenomic sequencing of viruses in clinical samples

... Results: In order to optimize metagenomic sequencing for application in virus diagnostics, we tested different enrichment and amplification procedures on plasma samples spiked with RNA and DNA viruses. A protocol ...

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Proteomic Biomarker Discovery: Review of the Science and Its Challenges with Clinical Samples

Proteomic Biomarker Discovery: Review of the Science and Its Challenges with Clinical Samples

... Cancer biomarkers discovered using cell lines and clinical samples with proteomic methods Biomarkers identified using breast clinical samples Protein tBID BcL-XL 14 3 3 theta/tau Ubiquit[r] ...

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Sequence Variation in the ftsZ Gene ofBartonella henselae Isolates and Clinical Samples

Sequence Variation in the ftsZ Gene ofBartonella henselae Isolates and Clinical Samples

... In this study, we have also used the ftsZ gene as a target for a diagnostic PCR with clinical samples, and we have identified B. henselae as the etiologic agent in two immunocompetent patients with ...

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Improved reduced representation bisulfite sequencing for epigenomic profiling of clinical samples

Improved reduced representation bisulfite sequencing for epigenomic profiling of clinical samples

... cell lines [8-10]. When it is required to analyze tens or hun- dreds of clinical samples, reduced representation bisulfite sequencing (RRBS) may be the method of choice [11]. The RRBS method makes use of ...

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Typing and subtyping of influenza viruses in clinical samples by PCR

Typing and subtyping of influenza viruses in clinical samples by PCR

... specimens. Clinical samples from patients with respiratory disease were obtained from the Laboratory Centre for Disease Control and from the Children’s Hospital of Eastern ...Ontario. Samples from ...

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Detection of Sporothrix schenckii in Clinical Samples by a Nested PCR Assay

Detection of Sporothrix schenckii in Clinical Samples by a Nested PCR Assay

... A common target of diagnostic fungal PCR assay is the 18S rRNA gene because its frequency in the genome yields a high sensitivity of the PCR (17). Although the 18S rRNA gene of S. schenckii is composed of many conserved ...

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METHICILIN RESISTANT STAPHYLOCOCCUS AUREUS IN CLINICAL SAMPLES AND FOMITES

METHICILIN RESISTANT STAPHYLOCOCCUS AUREUS IN CLINICAL SAMPLES AND FOMITES

... Two hundred (200) swab specimen used were collected randomly from apparently clinical samples of skin, nasal and fomites i.e laboratory coat from irrua specialist teaching hospital, Irrua, Edo State. Nasal ...

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The factor structure and psychometric properties of the Clinical Outcomes in Routine Evaluation – Outcome Measure (CORE OM) in Norwegian clinical and non clinical samples

The factor structure and psychometric properties of the Clinical Outcomes in Routine Evaluation – Outcome Measure (CORE OM) in Norwegian clinical and non clinical samples

... Results: The results indicate that the CORE-OM both measures general (g) psychological distress and sub-domains, of which risk of harm separates most clearly from the g factor. Internal consistency, stability and cut-off ...

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Multiprimer PCR system for differential identification of mycobacteria in clinical samples

Multiprimer PCR system for differential identification of mycobacteria in clinical samples

... A differential identification of the type of mycobacteria present in patients is also important for the control of the disease, since only then can a rapid initiation of effective an- tituberculosis treatment be ...

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Polymerase chain reaction for detection of Mycoplasma genitalium in clinical samples

Polymerase chain reaction for detection of Mycoplasma genitalium in clinical samples

... The amplified DNA fragments from all of our clinical samples possessed the AluI, Sau3AI, and DdeI restriction sites, but three samples from two patients did not contain the SspI site and[r] ...

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Identification of Paecilomyces variotii in Clinical Samples and Settings

Identification of Paecilomyces variotii in Clinical Samples and Settings

... Paecilomyces variotii is a commonly occurring species in air and food, but it is also associated with many types of human infections and is among the emerging causative agents of opportunistic mycoses in immunocom- ...

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Vancomycin Resistance Pattern of Staphylococcus Aureus among Clinical Samples

Vancomycin Resistance Pattern of Staphylococcus Aureus among Clinical Samples

... 1. Memariani M, Pourmand M, Shirazi M, Soltan Dallal MM, Abdossamadi Z, Mardani N. The importance of inducible clindamycin resistance in enterotoxin positive S. aureus isolated from clinical samples. Tehran ...

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Cladosporium Species Recovered from Clinical Samples in the United States

Cladosporium Species Recovered from Clinical Samples in the United States

... reported clinical cases (7, 13, 15, ...of clinical isolates, including several species obtained from diverse anatomical sites and not previously reported from clinical ...

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Development of Molecular Methods for Identification of Schizophyllum commune from Clinical Samples

Development of Molecular Methods for Identification of Schizophyllum commune from Clinical Samples

... in clinical samples and is thus unable to form the characteristic clamp connections and fruiting bodies ...from clinical samples, found 58% of the isolates to be mono- ...with clinical ...

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Novel Method for Detection, Typing, and Quantification of Human Papillomaviruses in Clinical Samples

Novel Method for Detection, Typing, and Quantification of Human Papillomaviruses in Clinical Samples

... Figure 2b shows a similar experiment with the HPV-18- positive HeLa cell line. Here, significant fluorescence was de- tected only with the Sc18 Scorpion. These primers were then used to detect HPV-16 and -18 in ...

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Performance of 6 HCV genotyping 9G test for HCV genotyping in clinical samples

Performance of 6 HCV genotyping 9G test for HCV genotyping in clinical samples

... Currently, various nucleic acid tests such real-time PCR [11, 12], restriction fragment length polymorphism [13], heteroduplex mobility analysis [14], and line-probe assay [15] are used to detect HCV genotypes in plasma ...

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Gambling risk and protective factors among community and clinical samples in Singapore

Gambling risk and protective factors among community and clinical samples in Singapore

... Thirdly, survey type questionnaires are generally prone to ‘socially desirable’ responses [39]. In this study, however, the survey method of assessment is relevant to the study’s research aims as the study aimed to ...

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Is Staphylococcus lugdunensis Significant in Clinical Samples?

Is Staphylococcus lugdunensis Significant in Clinical Samples?

... Staphylococcus lugdunensis grows under aerobic conditions on common media such as blood agar and causes intense beta-hemolysis. The traditionally used tube test and slide agglutination test are now obsolete due to their ...

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Use of immunomagnetic reduction for C-reactive protein assay in clinical samples

Use of immunomagnetic reduction for C-reactive protein assay in clinical samples

... Biomarkers play a role in the functioning of pathogens, and are used as indicators of disease in the clinical setting. For example, when tissues are damaged during the course of infectious and noninfectious ...

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