Cloning and Vector Construction
Designing and Construction of a Cloning Vector Containing mpt64 Gene of Mycobacterium tuberculosis
... pcDNA3.1 (+) vector, 2.5 μL T4 DNA ligase buffer, 0.2 μL T4 DNA ligase (150 U/μL) (Metabion, Germany) and 2 μL of the polyethylene glycol (PEG) 4000 (Thermo Scientific, Germany). After ligation, the competent ...
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Design and Construction of a Cloning Vector Containing the hspX Gene of Mycobacterium tuberculosis
... Background: Tuberculosis (TB) is a major cause of death worldwide. Finding an effective vaccine against TB is the best way to control it. Several vaccines against this disease have been developed but none are completely ...
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Construction of an insertional-inactivation cloning vector for Escherichia coli using a Rhodococcus gene for indigo production
... pSLHl ; ClaI/XbaI fragment of pKSmut (Fig. 1) containing the pigment gene, blunted and subcloned into the blunted SphIIHindIII sites of pUC18, showing rare codons (underlined), stop c[r] ...
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Construction of Double Gene Expression Vector
... larger vector. In order to overcome these problems a novel expression vector was developed with unique restriction sites and two different inducible promoters to control the expression of both the ...
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Rapid construction of mycobacterial mutagenesis vectors using ligation independent cloning
... Fig. 1. Illustration of how LIC works using the enzyme BsaI for the linearisation of the vector. The plasmid contains two BsaI sites that once digested will expose 3′ ends accessible to T 4 DNA polymerase. If only ...
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Development of a dual-expression vector facilitated with selection-free PCR recombination cloning strategy
... the construction of recombinant expression vector of a target gene includes PCR cloning and restriction enzyme mediated subcloning, which is time-consuming and sometimes troublesome because of the ...
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Uni-ZAP XR Vector Kit and Uni-ZAP XR Gigapack Cloning Kits
... XR Vector System The Uni-ZAP XR vector system combines the high efficiency of lambda library construction and the convenience of a plasmid system with blue- white color ...XR vector (Figure 1) ...
Comparative of Recombinant Vespa affinis Hyaluronidase Expressed in Different Cloning Vector and their Biological Properties
... easy vector into both pET-17b and pET-32a expres- sion ...pET-32a vector were digested with restriction enzymes KpnI and NotI using double diges- tion method at 37 ◦ C for 3 ...pET-32a-VesA2 ...
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Construction of Overexpression Vector of ACBP Gene in Zygosaccharomyces rouxii
... express vector can be trans- formed exogenous gene and using G418 to positive screening in wild type ...the vector which overexpression of ...expression vector with kanMX gene to obtain the G418 ...
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Transposon-Assisted Cloning and Traceless Mutagenesis of Adenoviruses: Development of a Novel Vector Based on Species D
... D vector has been reported yet ...BAC vector that carries only the Ad19a ...combinatorial construction of recombinant Ads (13), subclon- ing of large homology regions is not required for ITR-medi- ...
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QuickStep-Cloning: a sequence-independent, ligation-free method for rapid construction of recombinant plasmids
... megaprimer-based cloning methods. However, what about more popular cloning methods such Gibson Assembly cloning [18, 19] or Ligation Independent Cloning (LIC) [20]? Both of these methods are ...
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Cloning Of Anopheles Gambiae CYP6M2 Gene Promoter And Construction Of Its Luciferase Reporter System
... expression vector pGL3-Basic ...and construction of its dual luciferase reporter system were successfully established, this will be essential in further studies aimed at understanding the regulatory ...
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Construction and Characterization of a Replication-Competent Retroviral Shuttle Vector Plasmid
... a vector system that makes it simple to recover both unintegrated and integrated ...the cloning of unintegrated viral DNA was more-than-100-fold more efficient when the DNA was intro- duced into ...
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Construction, characterisation and utilisation of a novel E coli expression vector
... this cloning step it became apparent that the annealed oligonucleotides were capable of being inserted in either ...derived vector was the only orientation capable of producing a functional alpha-peptide, ...
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Construction of GmNAC085 vector for future development of drought-tolerant crops
... Successful cloning of the GmNAC085 genes into binary vector and successful transformation of the recombinant vector into ...binary vector pBI121 using the same restriction enzymes XbaI and ...
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Choosing a Cloning Vector
... The bacteriophage λ genome comprises 48,502 bp. On entering the host cell, the phage adopts one of two life cycles: lytic growth or lysogeny. In lytic growth, approx 100 new virions are synthesized and packaged before ...
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pELMO, an optimised in-house cloning vector
... Easy vector cloning efficiency may thus be under-estimated as false negative colonies may result from unexpected PCR fragments cloned in-frame within the lacZ gene when cloning DNA fragments up to 2 ...
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mirnaselect pep-mir Cloning and Expression Vector
... 1. miRNASelect™ pEP-miR Cloning and Expression Vector (Part No. MIR-EXP): One tube 2. miRNASelect™ pEP-miR Null Control Vector (Part No. MIR-NULL): One tube Background MicroRNAs (miRNAs) are 18–24 ...
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Cloning Nanobody cDNA into pHEN6c Plasmid Vector
... gene cloning. The term “gene cloning” covers a wide range of techniques that make it possible to manipulate DNA in a test tube and to return it to living organisms where it functions ...Gene cloning ...
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mirnaselect pegp-mir Cloning and Expression Vector
... Synthetic miRNA molecules based on predicted mature miRNA sequence are sometimes used. Despite their optimized design criteria, synthetic miRNAs underscore the importance of primary miRNA in its native expressed form. ...
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