Cobas AmpliPrep
Development of a Second Version of the Cobas AmpliPrep/Cobas TaqMan Hepatitis C Virus Quantitative Test with Improved Genotype Inclusivity
... the Cobas AmpliPrep/Cobas TaqMan HCV Quantitative Test (CAP/CTM v2) with decreased sample input volume and improved genotype inclusivity was ...the Cobas AmpliPrep/Cobas TaqMan ...
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Performance of Three Commercial Viral Load Assays, Versant Human Immunodeficiency Virus Type 1 (HIV 1) RNA bDNA v3 0, Cobas AmpliPrep/Cobas TaqMan HIV 1, and NucliSens HIV 1 EasyQ v1 2, Testing HIV 1 Non B Subtypes and Recombinant Variants
... Monitoring antiretroviral therapy requires that human immunodeficiency virus type 1 (HIV-1) viremia assays are applicable to all distinct variants. This study evaluates the performance of three commercial viral load ...
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Analytical characteristics and comparative evaluation of Aptima HCV quant Dx assay with the Abbott RealTime HCV assay and Roche COBAS AmpliPrep/COBAS TaqMan HCV quantitative test v2 0
... Methods: The analytical sensitivity, analytical specificity, precision, and linearity of the Aptima assay were assessed. The performance of the Aptima assay was compared to two commercially available HCV assays; the ...
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Abbott RealTime Hepatitis C Virus (HCV) and Roche Cobas AmpliPrep/Cobas TaqMan HCV Assays for Prediction of Sustained Virological Response to Pegylated Interferon and Ribavirin in Chronic Hepatitis C Patients
... The quantification of hepatitis C virus (HCV) RNA is es- sential for the management of chronic hepatitis C therapy based on the combination of pegylated interferon (PEG-IFN) and ribavirin (RBV). Based on pivotal trials ...
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Evaluation of the Roche Cobas AmpliPrep/Cobas TaqMan HIV 1 Test and Identification of Rare Polymorphisms Potentially Affecting Assay Performance
... Versions of the CAP/CTM product insert subsequent to the investigation of the discrepant samples described in this study have included a statement that “though rare, mutations within the highly conserved region of the ...
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Ability of Two Commercially Available Assays (Abbott RealTime HIV 1 and Roche Cobas AmpliPrep/Cobas TaqMan HIV 1 Version 2 0) To Quantify Low HIV 1 RNA Levels ( Alessandra Amendola, Patrizia Marsella, Maria Bloisi, Federica Forbici, Claudio Angeletti, Maria R Capobianchi A M Caliendo, Editor
... Concordance between molecular assays may be suboptimal at low HIV-1 viremia levels (<1,000 copies/ml); therefore, it may be difficult to define and compare virologic endpoints for successful and failed therapy. We ...
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Performance of the Cobas AmpliPrep/Cobas TaqMan Real Time PCR Assay for Hepatitis B Virus DNA Quantification
... Hepatitis B virus (HBV) DNA quantification is used to establish the prognosis of chronic HBV-related liver disease, to identify those patients who need to be treated, and to monitor the virologic response and resistance ...
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Dynamic Range of Hepatitis C Virus RNA Quantification with the Cobas Ampliprep Cobas Amplicor HCV Monitor v2 0 Assay
... Accurate quantification of hepatitis C virus (HCV) RNA is needed in clinical practice to decide whether to continue or stop pegylated interferon- ␣ -ribavirin combination therapy at week 12 of treatment for patients with ...
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Performance of Version 2 0 of the Cobas AmpliPrep/Cobas TaqMan Real Time PCR Assay for Hepatitis B Virus DNA Quantification
... the Cobas AmpliPrep/Cobas TaqMan (CAP/CTM) assay, a fully automated platform for HBV DNA quantification in serum or in plasma with a claimed lower limit of detection of 20 IU/ml and a claimed upper ...
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Second Generation Cobas AmpliPrep/Cobas TaqMan HCV Quantitative Test for Viral Load Monitoring: a Novel Dual Probe Assay Design
... Hepatitis C virus (HCV) RNA viral load (VL) monitoring is a well-established diagnostic tool for the management of chronic hepatitis C patients. HCV RNA VL results are used to make treatment decisions with the goal of ...
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Comparison of an In House Quantitative Real Time PCR and COBAS AmpliPrep/TaqMan Roche for Determination of Viral Load for HIV Type 1 Non B
... Only 4 samples had discordant VL with the 2 techniques used. Indeed, two samples of subtype K (−1.13) and C (−0.56) were overestimated by the COBAS AmpliPrep/TaqMan and two others, subtype G (0.57) and A ...
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COBAS AmpliPrep COBAS TaqMan Hepatitis B Virus (HBV) Test: a Novel Automated Real Time PCR Assay for Quantification of HBV DNA in Plasma
... system COBAS AmpliPrep-COBAS TaqMan 48 (CAP-CTM; Roche, Branchburg, NJ) for HBV DNA extraction and real-time PCR quantification were assessed and compared to the endpoint PCR COBAS AMPLICOR ...
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Performance Evaluation of the New Roche cobas AmpliPrep/cobas TaqMan HCV Test, Version 2 0, for Detection and Quantification of Hepatitis C Virus RNA
... To evaluate the analytical performance and explore the clinical applicability of the new Roche cobas AmpliPrep/cobas TaqMan HCV test, v2.0 (CAP/CTM v2.0), a platform comparison was performed on ...
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Correction of Underquantification of Human Immunodeficiency Virus Type 1 Load with the Second Version of the Roche Cobas AmpliPrep/Cobas TaqMan Assay
... the Cobas AmpliPrep of Roche Diagnostics and M1000 of Abbott Diagnostics, Abbott Park, IL) facilitated high-volume testing and made viral load testing more ...Roche Cobas ...
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Performance Evaluation of the New Roche Cobas AmpliPrep/Cobas TaqMan HIV 1 Test Version 2 0 for Quantification of Human Immunodeficiency Virus Type 1 RNA
... Roche COBAS AmpliPrep COBAS TaqMan HIV-1 test, version ...the COBAS Amplicor HIV-1 Monitor Test, version 1.5 (CAP/CA v1.5), the COBAS AmpliPrep COBAS TaqMan HIV-1 Test ...
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The Cobas AmpliPrep/Cobas TaqMan HCV Test, Version 2 0, Real Time PCR Assay Accurately Quantifies Hepatitis C Virus Genotype 4 RNA
... A second generation of the CAP/CTM assay for HCV RNA, the Cobas AmpliPrep/Cobas TaqMan HCV test, version 2.0 (CAP/ CTM HCV v2.0), has been recently released. Several changes have been made, including ...
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Evaluation of the Abbott m2000 RealTime Human Immunodeficiency Virus Type 1 (HIV 1) Assay for HIV Load Monitoring in South Africa Compared to the Roche Cobas AmpliPrep Cobas Amplicor, Roche Cobas AmpliPrep Cobas TaqMan HIV 1, and BioMerieux NucliSENS EasyQ HIV 1 Assays
... The first three assays mentioned above use real-time plat- forms with fluorescence detection systems, and CAP/CA HIV-1 is based on endpoint detection. Previous publications reported on the performance of CAP/CTM HIV-1 ...
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Comparison of the Abbott RealTime Human Immunodeficiency Virus Type 1 (HIV 1) Assay to the Cobas AmpliPrep/Cobas TaqMan HIV 1 Test: Workflow, Reliability, and Direct Costs
... Clinical specimens. Aliquots of HIV-1 RNA-positive EDTA-plasma speci- mens obtained from 108 patients and previously submitted to the Hepatitis/HIV Molecular Laboratory at Mayo Clinic Rochester for HIV-1 viral load ...
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Analytical characteristics and comparative evaluation of Aptima HIV 1 Quant Dx assay with Ampliprep/COBAS TaqMan HIV 1 test v2 0
... Abbott RT: Abbott RealTi m e; AIDS: Acquired immune deficiency syndrome; Aptima Assay: Aptima HIV-1 Quant Dx assay; CRF: Circulating recombinant forms; CV: Coefficient of variation; HIV: Human immunodeficiency virus; ...
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Evaluation of the Aptima HCV Quant Dx Assay Using Serum and Dried Blood Spots
... ABSTRACT Hepatitis C virus (HCV) RNA quantitation is the primary method by which active HCV infections are identified and the response to direct-acting antiviral therapy is monitored. This study describes the evaluation ...
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