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COBAS AmpliPrep/TaqMan

Ability of Two Commercially Available Assays (Abbott RealTime HIV 1 and Roche Cobas AmpliPrep/Cobas TaqMan HIV 1 Version 2 0) To Quantify Low HIV 1 RNA Levels (  
    	 Alessandra Amendola, Patrizia Marsella, Maria Bloisi, Federica Forbici, Claudio Angele

Ability of Two Commercially Available Assays (Abbott RealTime HIV 1 and Roche Cobas AmpliPrep/Cobas TaqMan HIV 1 Version 2 0) To Quantify Low HIV 1 RNA Levels ( Alessandra Amendola, Patrizia Marsella, Maria Bloisi, Federica Forbici, Claudio Angeletti, Maria R Capobianchi A M Caliendo, Editor

... Concordance between molecular assays may be suboptimal at low HIV-1 viremia levels (<1,000 copies/ml); therefore, it may be difficult to define and compare virologic endpoints for successful and failed therapy. We ...

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Comparison of an In House Quantitative Real Time PCR and COBAS AmpliPrep/TaqMan Roche for Determination of Viral Load for HIV Type 1 Non B

Comparison of an In House Quantitative Real Time PCR and COBAS AmpliPrep/TaqMan Roche for Determination of Viral Load for HIV Type 1 Non B

... Our study demonstrates that there is no significant difference between the results of Viral Loads (VL) deter- mined by the COBAS AmpliPrep/TaqMan technology and the in-house technique used. It can be ...

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Performance of Three Commercial Viral Load Assays, Versant Human Immunodeficiency Virus Type 1 (HIV 1) RNA bDNA v3 0, Cobas AmpliPrep/Cobas TaqMan HIV 1, and NucliSens HIV 1 EasyQ v1 2, Testing HIV 1 Non B Subtypes and Recombinant Variants

Performance of Three Commercial Viral Load Assays, Versant Human Immunodeficiency Virus Type 1 (HIV 1) RNA bDNA v3 0, Cobas AmpliPrep/Cobas TaqMan HIV 1, and NucliSens HIV 1 EasyQ v1 2, Testing HIV 1 Non B Subtypes and Recombinant Variants

... v3.0, Cobas AmpliPrep/Cobas TaqMan HIV-1, and NucliSens HIV-1 EasyQ ...or TaqMan tests, respectively, suggesting potential viral load underestimation of some samples by all ...versus ...

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Evaluation of the Roche Cobas AmpliPrep/Cobas TaqMan HIV 1 Test and Identification of Rare Polymorphisms Potentially Affecting Assay Performance

Evaluation of the Roche Cobas AmpliPrep/Cobas TaqMan HIV 1 Test and Identification of Rare Polymorphisms Potentially Affecting Assay Performance

... Roche Cobas AmpliPrep/Cobas TaqMan (CAP/CTM) HIV-1 viral load assay for sensitivity, reproducibility, linearity, HIV-1 subtype detection, and correlation to the Roche Amplicor HIV-1 monitor ...

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Performance Evaluation of the New Roche cobas AmpliPrep/cobas TaqMan HCV Test, Version 2 0, for Detection and Quantification of Hepatitis C Virus RNA

Performance Evaluation of the New Roche cobas AmpliPrep/cobas TaqMan HCV Test, Version 2 0, for Detection and Quantification of Hepatitis C Virus RNA

... To evaluate the analytical performance and explore the clinical applicability of the new Roche cobas AmpliPrep/cobas TaqMan HCV test, v2.0 (CAP/CTM v2.0), a platform comparison was performed ...

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Correction of Underquantification of Human Immunodeficiency Virus Type 1 Load with the Second Version of the Roche Cobas AmpliPrep/Cobas TaqMan Assay

Correction of Underquantification of Human Immunodeficiency Virus Type 1 Load with the Second Version of the Roche Cobas AmpliPrep/Cobas TaqMan Assay

... (iii) Cobas AmpliPrep/Cobas TaqMan HIV-1 test, version 2.0 (CAP/CTM v2.0; Roche Diagnostics Ltd.). This assay simultaneously targets the gag and the ltr region with two dually labeled ...

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Analytical characteristics and comparative evaluation of Aptima HCV quant Dx assay with the Abbott RealTime HCV assay and Roche COBAS AmpliPrep/COBAS TaqMan HCV quantitative test v2 0

Analytical characteristics and comparative evaluation of Aptima HCV quant Dx assay with the Abbott RealTime HCV assay and Roche COBAS AmpliPrep/COBAS TaqMan HCV quantitative test v2 0

... Methods: The analytical sensitivity, analytical specificity, precision, and linearity of the Aptima assay were assessed. The performance of the Aptima assay was compared to two commercially available HCV assays; the ...

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Development of a Second Version of the Cobas AmpliPrep/Cobas TaqMan Hepatitis C Virus Quantitative Test with Improved Genotype Inclusivity

Development of a Second Version of the Cobas AmpliPrep/Cobas TaqMan Hepatitis C Virus Quantitative Test with Improved Genotype Inclusivity

... The study of HCV replication and of its kinetics during antiviral therapy with pegylated interferon and ribavirin has led to the identification of distinct response patterns that serve to individualize treatment ...

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Performance of the Cobas AmpliPrep/Cobas TaqMan Real Time PCR Assay for Hepatitis B Virus DNA Quantification

Performance of the Cobas AmpliPrep/Cobas TaqMan Real Time PCR Assay for Hepatitis B Virus DNA Quantification

... Hepatitis B virus (HBV) DNA quantification is used to establish the prognosis of chronic HBV-related liver disease, to identify those patients who need to be treated, and to monitor the virologic response and resistance ...

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Evaluation of the COBAS AmpliPrep Total Nucleic Acid Isolation COBAS TaqMan Hepatitis B Virus (HBV) Quantitative Test and Comparison to the VERSANT HBV DNA 3 0 Assay

Evaluation of the COBAS AmpliPrep Total Nucleic Acid Isolation COBAS TaqMan Hepatitis B Virus (HBV) Quantitative Test and Comparison to the VERSANT HBV DNA 3 0 Assay

... The COBAS TaqMan HBV test (CTM HBV test; Roche Diagnostics, Meylan, France) is a recent commercial nucleic acid amplification test for HBV DNA viral load determination based on TaqMan PCR chemistry ...

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COBAS AmpliPrep COBAS TaqMan Hepatitis B Virus (HBV) Test: a Novel Automated Real Time PCR Assay for Quantification of HBV DNA in Plasma

COBAS AmpliPrep COBAS TaqMan Hepatitis B Virus (HBV) Test: a Novel Automated Real Time PCR Assay for Quantification of HBV DNA in Plasma

... the COBAS AmpliPrep-COBAS TaqMan HBV test (CAP-CTM; Roche Molecular Systems, ...the COBAS AmpliPrep for automated nucleic acid extraction from plasma specimens and the ...

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Second Generation Cobas AmpliPrep/Cobas TaqMan HCV Quantitative Test for Viral Load Monitoring: a Novel Dual Probe Assay Design

Second Generation Cobas AmpliPrep/Cobas TaqMan HCV Quantitative Test for Viral Load Monitoring: a Novel Dual Probe Assay Design

... by Cobas AmpliPrep, followed by amplifica- tion with primers specific to the 5= UTR of the HCV genome and detection with a fluorescently labeled hydrolysis probe performed with the Cobas ...

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Performance Evaluation of the New Roche Cobas AmpliPrep/Cobas TaqMan HIV 1 Test Version 2 0 for Quantification of Human Immunodeficiency Virus Type 1 RNA

Performance Evaluation of the New Roche Cobas AmpliPrep/Cobas TaqMan HIV 1 Test Version 2 0 for Quantification of Human Immunodeficiency Virus Type 1 RNA

... (COBAS AmpliPrep CO- BAS TaqMan HIV-1 Test, version ...the COBAS TaqMan platform ...the COBAS Amplicor HIV-1 Monitor Test, version ...the COBAS AmpliPrep ...

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The Cobas AmpliPrep/Cobas TaqMan HCV Test, Version 2 0, Real Time PCR Assay Accurately Quantifies Hepatitis C Virus Genotype 4 RNA

The Cobas AmpliPrep/Cobas TaqMan HCV Test, Version 2 0, Real Time PCR Assay Accurately Quantifies Hepatitis C Virus Genotype 4 RNA

... A second generation of the CAP/CTM assay for HCV RNA, the Cobas AmpliPrep/Cobas TaqMan HCV test, version 2.0 (CAP/ CTM HCV v2.0), has been recently released. Several changes have been made, ...

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Performance of Version 2 0 of the Cobas AmpliPrep/Cobas TaqMan Real Time PCR Assay for Hepatitis B Virus DNA Quantification

Performance of Version 2 0 of the Cobas AmpliPrep/Cobas TaqMan Real Time PCR Assay for Hepatitis B Virus DNA Quantification

... quantification of HBV DNA is recommended, because of their excellent analytical sensitivity (lower limit of detection, 10 to 20 IU/ml), their specificity, their accuracy, and their broad dynamic range of linear ...

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Evaluation of the Abbott m2000 RealTime Human Immunodeficiency Virus Type 1 (HIV 1) Assay for HIV Load Monitoring in South Africa Compared to the Roche Cobas AmpliPrep Cobas Amplicor, Roche Cobas AmpliPrep Cobas TaqMan HIV 1, and BioMerieux NucliSENS Easy

Evaluation of the Abbott m2000 RealTime Human Immunodeficiency Virus Type 1 (HIV 1) Assay for HIV Load Monitoring in South Africa Compared to the Roche Cobas AmpliPrep Cobas Amplicor, Roche Cobas AmpliPrep Cobas TaqMan HIV 1, and BioMerieux NucliSENS EasyQ HIV 1 Assays

... the TaqMan 48 instrument was used in this study, a TaqMan 96 instrument is available on the market, and that instrument would generate 84 patient results per run (includ- ing 12 controls per batch for a ...

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Comparison of the RealTime HIV 1, COBAS TaqMan 48 v1 0, Easy Q v1 2, and Versant v3 0 assays for Determination of HIV 1 Viral Loads in a Cohort of Canadian Patients with Diverse HIV Subtype Infections

Comparison of the RealTime HIV 1, COBAS TaqMan 48 v1 0, Easy Q v1 2, and Versant v3 0 assays for Determination of HIV 1 Viral Loads in a Cohort of Canadian Patients with Diverse HIV Subtype Infections

... HIV clinics in Canada provide care to an increasing number of patients born outside of Canada with HIV-1 non-B subtype infections. Because the Easy Q HIV-1 v1.2 assay (EQ; bioMe ´rieux) failed to detect some non-B ...

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Comparison of the Abbott RealTime Human Immunodeficiency Virus Type 1 (HIV 1) Assay to the Cobas AmpliPrep/Cobas TaqMan HIV 1 Test: Workflow, Reliability, and Direct Costs

Comparison of the Abbott RealTime Human Immunodeficiency Virus Type 1 (HIV 1) Assay to the Cobas AmpliPrep/Cobas TaqMan HIV 1 Test: Workflow, Reliability, and Direct Costs

... Clinical specimens. Aliquots of HIV-1 RNA-positive EDTA-plasma speci- mens obtained from 108 patients and previously submitted to the Hepatitis/HIV Molecular Laboratory at Mayo Clinic Rochester for HIV-1 viral load ...

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Evaluation of the Aptima HIV 1 Quant Dx Assay Using Plasma and Dried Blood Spots

Evaluation of the Aptima HIV 1 Quant Dx Assay Using Plasma and Dried Blood Spots

... HIV-1 RNA quantitation in plasma, or virus load testing, is the primary method by which the response to antiretroviral therapy is monitored. Here we describe evaluation of the Aptima HIV-1 Quant Dx assay (Aptima) ...

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Molecular diagnostics in the management of chronic hepatitis C: key considerations in the era of new antiviral therapies

Molecular diagnostics in the management of chronic hepatitis C: key considerations in the era of new antiviral therapies

... The COBAS ® AmpliPrep / COBAS ® TaqMan ® HCV Quantitative and Qualitative Test, version ...the COBAS AmpliPrep platform followed by HCV tar- get specific (5’ UTR) amplification ...

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