Cobas Taqman
COBAS AmpliPrep COBAS TaqMan Hepatitis B Virus (HBV) Test: a Novel Automated Real Time PCR Assay for Quantification of HBV DNA in Plasma
... the COBAS AmpliPrep-COBAS TaqMan HBV test (CAP-CTM; Roche Molecular Systems, ...the COBAS AmpliPrep for automated nucleic acid extraction from plasma specimens and the COBAS ...
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Evaluation of the Roche Cobas AmpliPrep/Cobas TaqMan HIV 1 Test and Identification of Rare Polymorphisms Potentially Affecting Assay Performance
... Versions of the CAP/CTM product insert subsequent to the investigation of the discrepant samples described in this study have included a statement that “though rare, mutations within the highly conserved region of the ...
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Evaluation of the COBAS TaqMan HCV Test with Automated Sample Processing Using the MagNA Pure LC Instrument
... controls, TaqMan HCV is a complete test kit currently designated for research use only in the United ...Currently, TaqMan HCV is comprised of two products, the High Pure System viral nucleic acid kit (Roche ...
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Ability of Two Commercially Available Assays (Abbott RealTime HIV 1 and Roche Cobas AmpliPrep/Cobas TaqMan HIV 1 Version 2 0) To Quantify Low HIV 1 RNA Levels ( Alessandra Amendola, Patrizia Marsella, Maria Bloisi, Federica Forbici, Claudio Angeletti, Maria R Capobianchi A M Caliendo, Editor
... The Roche assay (Roche Cobas AmpliPrep/Cobas TaqMan HIV-1 ver- sion 2.0, Roche Molecular Systems, Inc., Pleasanton, CA, USA) was per- formed on the docked configuration of the ...
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The Cobas AmpliPrep/Cobas TaqMan HCV Test, Version 2 0, Real Time PCR Assay Accurately Quantifies Hepatitis C Virus Genotype 4 RNA
... units/ml in a number of patients infected with HCV genotype 4 and occasionally failed to detect it. The aim of this study was to evaluate the ability of the Cobas AmpliPrep/Cobas TaqMan HCV test, ...
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Analytical characteristics and comparative evaluation of Aptima HCV quant Dx assay with the Abbott RealTime HCV assay and Roche COBAS AmpliPrep/COBAS TaqMan HCV quantitative test v2 0
... Methods: The analytical sensitivity, analytical specificity, precision, and linearity of the Aptima assay were assessed. The performance of the Aptima assay was compared to two commercially available HCV assays; the ...
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Evaluation of the Cobas TaqMan MTB Test for Direct Detection of Mycobacterium tuberculosis Complex in Respiratory Specimens
... the Cobas TaqMan MTB test was comparable to that of the replaced Cobas Amplicor MTB ...the Cobas TaqMan MTB kit are packaged in 12-test, single use ...
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Second Generation Cobas AmpliPrep/Cobas TaqMan HCV Quantitative Test for Viral Load Monitoring: a Novel Dual Probe Assay Design
... by Cobas AmpliPrep, followed by amplifica- tion with primers specific to the 5= UTR of the HCV genome and detection with a fluorescently labeled hydrolysis probe performed with the Cobas TaqMan ...
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Evaluation of Cobas TaqMan MTB for Direct Detection of the Mycobacterium tuberculosis Complex in Comparison with Cobas Amplicor MTB
... design. Inhibition of the PCR, as indicated by a negative internal control as used in the M. tuberculosis assay, occurred for 34 (3.0%) of the specimens. None of the PCR-inhibited samples was smear positive and/or M. ...
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Performance of the Cobas AmpliPrep/Cobas TaqMan Real Time PCR Assay for Hepatitis B Virus DNA Quantification
... Hepatitis B virus (HBV) DNA quantification is used to establish the prognosis of chronic HBV-related liver disease, to identify those patients who need to be treated, and to monitor the virologic response and resistance ...
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Performance of Three Commercial Viral Load Assays, Versant Human Immunodeficiency Virus Type 1 (HIV 1) RNA bDNA v3 0, Cobas AmpliPrep/Cobas TaqMan HIV 1, and NucliSens HIV 1 EasyQ v1 2, Testing HIV 1 Non B Subtypes and Recombinant Variants
... v3.0, Cobas AmpliPrep/Cobas TaqMan HIV-1, and NucliSens HIV-1 EasyQ ...or TaqMan tests, respectively, suggesting potential viral load underestimation of some samples by all ...versus ...
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Correction of Underquantification of Human Immunodeficiency Virus Type 1 Load with the Second Version of the Roche Cobas AmpliPrep/Cobas TaqMan Assay
... To overcome the issue of underquantification, the CAP/ CTM assay was modified to Cobas AmpliPrep/Cobas TaqMan HIV-1 test, version 2.0 (CAP/CTM v2.0). A dual-target strat- egy was chosen: besides gag ...
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Development of a Second Version of the Cobas AmpliPrep/Cobas TaqMan Hepatitis C Virus Quantitative Test with Improved Genotype Inclusivity
... the Cobas AmpliPrep/Cobas TaqMan HCV Quantitative Test (CAP/CTM v2) with decreased sample input volume and improved genotype inclusivity was ...the Cobas AmpliPrep/Cobas TaqMan ...
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Performance of Version 2 0 of the Cobas AmpliPrep/Cobas TaqMan Real Time PCR Assay for Hepatitis B Virus DNA Quantification
... quantification of HBV DNA is recommended, because of their excellent analytical sensitivity (lower limit of detection, 10 to 20 IU/ml), their specificity, their accuracy, and their broad dynamic range of linear ...
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Performance Evaluation of the New Roche cobas AmpliPrep/cobas TaqMan HCV Test, Version 2 0, for Detection and Quantification of Hepatitis C Virus RNA
... To evaluate the analytical performance and explore the clinical applicability of the new Roche cobas AmpliPrep/cobas TaqMan HCV test, v2.0 (CAP/CTM v2.0), a platform comparison was performed on ...
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Performance Evaluation of the New Roche Cobas AmpliPrep/Cobas TaqMan HIV 1 Test Version 2 0 for Quantification of Human Immunodeficiency Virus Type 1 RNA
... Roche COBAS AmpliPrep COBAS TaqMan HIV-1 test, version ...the COBAS Amplicor HIV-1 Monitor Test, version 1.5 (CAP/CA v1.5), the COBAS AmpliPrep COBAS TaqMan HIV-1 Test ...
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Comparison of the RealTime HIV 1, COBAS TaqMan 48 v1 0, Easy Q v1 2, and Versant v3 0 assays for Determination of HIV 1 Viral Loads in a Cohort of Canadian Patients with Diverse HIV Subtype Infections
... HIV clinics in Canada provide care to an increasing number of patients born outside of Canada with HIV-1 non-B subtype infections. Because the Easy Q HIV-1 v1.2 assay (EQ; bioMe ´rieux) failed to detect some non-B ...
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Comparison of the Abbott RealTime Human Immunodeficiency Virus Type 1 (HIV 1) Assay to the Cobas AmpliPrep/Cobas TaqMan HIV 1 Test: Workflow, Reliability, and Direct Costs
... Clinical specimens. Aliquots of HIV-1 RNA-positive EDTA-plasma speci- mens obtained from 108 patients and previously submitted to the Hepatitis/HIV Molecular Laboratory at Mayo Clinic Rochester for HIV-1 viral load ...
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Evaluation of the COBAS Hepatitis C Virus (HCV) TaqMan Analyte Specific Reagent Assay and Comparison to the COBAS Amplicor HCV Monitor V2 0 and Versant HCV bDNA 3 0 Assays
... Calibration. The TM-ASR assay requires user calibration of the assay with each new lot of reagents. Calibrators are not supplied with the TM-ASR reagents and are not available directly from the manufacturer. Calibrators ...
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Molecular diagnostics in the management of chronic hepatitis C: key considerations in the era of new antiviral therapies
... The COBAS ® AmpliPrep / COBAS ® TaqMan ® HCV Quantitative and Qualitative Test, version ...the COBAS AmpliPrep platform followed by HCV tar- get specific (5’ UTR) amplification and detection ...
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